排序方式： 共有9条查询结果，搜索用时 15 毫秒
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采用PCR扩增的方法获得了第一个松藻目物种长松藻(Codium cylindricum)的ITS序列,长松藻ITS全长515bp,其中ITS1的长度为74bp,5.8S序列长163bp,ITS2的长度为278bp.与已知的绿藻门其它5个目8个属物种的ITS序列的系统发育分析结果表明,绿藻门物种分为三大支,其中团藻目藻类为一支,松藻目和刚毛藻目藻类为一支,石莼目、顶管藻目和丝藻目藻类构成一大支;长松藻与刚毛藻目的刚毛藻属和硬毛藻属物种同为多核藻体,在系统发生上表现出更近的亲缘关系;不同绿藻目海藻的聚类关系表明,其能够准确地反映出其在单核与多核、单细胞与多细胞等生物结构特性方面的差异与水平. 相似文献
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磷酸甘露糖异构酶基因(phosphomannose isomerase,PMI)编码磷酸甘露糖异构酶(PMI),可逆催化甘露糖-6-磷酸和果糖-6-磷酸之间的相互转化,在GDP-D-甘露糖的合成中起重要的作用,后者是生物体合成糖蛋白和糖脂必需的前体物质。本文根据在Genbank中已经登录的部分藻类PMI基因序列,与部分细菌、真菌、植物和动物的PMI基因序列做比较,研究其进化趋势和规律及其基因结构,为以后在大型藻类中研究该基因奠定基础。系统进化分析表明,藻类的PMI基因有2个进化方向,绿藻类的PMI基因与高等植物的进化趋势一致;而杂色藻类(硅藻和褐藻)则单独形成一个进化分支。多序列比对表明,磷酸甘露糖异构酶蛋白有3个保守区,其中有4个氨基酸残基位点是金属离子结合位点,分别为2个Glu和2个His。对部分藻类的PMI基因进行结构分析表明:在比较低等的藻类中,PMI基因没有内含子插入,而较高等的藻类中则有不同数目和长度的内含子插入。对部分藻类PMI基因的生物信息学分析结果显示,PMI基因编码的蛋白为酸性蛋白,分子量在40到60kDa之间;二级结构中均以α-螺旋和无规则卷曲为主;三级结构与二级结构相吻合。 相似文献
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Ulva species can grow rapidly in nutrient-rich habitats causing green tides and marine fouling. A more complete understanding of the reasons behind these outbreaks is urgently required. Accordingly, this study attempts to use microsatellite markers based expressed sequence tag（EST） to analyze the genetic variation of several Ulva prolifera populations in the South Yellow Sea of China. Two hundred and thirty-eight SSRs were identified from 8 179 unique ESTs（6 203 newly sequenced and 1 976 downloaded from NCBI database） and 37 primer pairs were successfully designed according to the ESTs; 11 pairs were selected to detect the genetic diversity and relationship of 69 attached U. prolifera samples and 13 free-floating samples collected from coastal and off-coast areas of the South Yellow Sea. The results of cross-species transferability showed that six of the 11 EST-SSR primers could give good amplification in other five Ulva species and the average allele number was 4.67. Genetic variation analysis indicated that all 82 U. prolifera samples were clearly divided and most samples collected from the same site clustered together as a group in the dendrogram tree produced by unweighted pair-group mean analysis（UPGMA） method and the cluster results showed some consistency with the geographical origins. In addition, 13 free-floating samples（except HT-001-2） were grouped as a single clade separated from the attached samples. 相似文献
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通过PCR扩增基因片段的方法测定了红翎菜科琼枝属(Betaphycus)、卡帕藻属(Kappaphycus)和麒麟菜属(Eucheuma)4种海藻共19株个体的核糖体基因转录间隔区(ITS)(含5.8SrDNA)和核酮糖-1,5-二磷酸羧化酶/加氧酶基因大亚基(rbcL)全长基因序列。其中rbcL全长序列为首次测定,为从蛋白质水平探讨红翎菜科分子系统进化提供了可靠的保证。琼枝属、卡帕藻属、麒麟菜属ITS序列长度分别为1 024、629~669、1 001bp,GC含量在45.6%~52%之间;rbcL序列长度均为1 467bp,GC含量在37.1%~37.6%之间。结合GenBank中现有的红翎菜科海藻ITS和rbcL序列进行系统进化分析,2个片段聚类结果均明显的将所有样品分为琼枝属、卡帕藻属和麒麟菜属3大分支,表现出明显的属间差异。本研究的11株长心卡帕藻根据ITS序列差异分成明显2种类型,推测这2种类型长心卡帕藻的ITS序列差异与其地理环境、无性繁殖时间(代数)和藻体颜色无关。 相似文献
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In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase and the osmotic stabilizer was 2.0 mol/L glucose. The optimum enzymic hydrolysis conditions were found to be dark enzymolysis at 30°C continuing for 4.0 h. The resultant density and yield of protoplasts achieved 32.60×104 mL-1, 65.20×104 g-1 tissue for Kappaphycus alvarezii. Finally, under the temperature of 20°C, light intensity of 1 500–2 000 lx and photoperiod of 12 h/d, two developmental pathways were investigated:(1) callus-like cell mass and regenerated plantlet occurred on protoplast;(2) young shoots and calluslike cell mass occurred in tissue blocks after enzymolysis. 相似文献
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Restriction site amplified polymorphism (RSAP) was used, for the first time, to analyze the genetic structure and diversity of four, mainly cultivated, varieties of the brown alga, Saccharina japonica. Eighty-eight samples from varieties "Rongfu", "Fujian", "Ailunwan" and "Shengchanzhong" were used for the genetic analyses. One hundred and ninety-eight bands were obtained using eight combinations of primers. One hundred and ninety-one (96.46%) were polymorphic bands. Nei’s genetic diversity was 0.360, and the coefficient of genetic differentiation was 0.357. No inbreeding-type recession was found in the four brown alga varieties and the results of the "Ailunwan" variety using samples from 2 years showed that the variety was becoming less diverse during the selection inherent in the breeding program. Genetic diversity and cluster analyses results were consistent with these genetic relationships. The results show the RSAP method is suitable for genetic analysis. Continuous inbreeding and selection could reduce the genetic diversity effectively; therefore periodical supervision is required. 相似文献
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通过生殖细胞观察以及DNA含量检测研究南黄海浒苔(Ulva prolifera)群体的世代结构,发现近岸和近海漂浮浒苔群体均由孢子体世代和配子体世代构成,呈现混合世代群体结构。利用完全随机实验设计研究温度、光照强度和盐度对浒苔孢子体和配子体生殖的影响,发现3个生态因子对浒苔配子体和孢子体生殖的影响存在交互效应,在温度25℃、盐度40和光照7 000lx条件下,在24～48h内可促进配子体和孢子体的生殖发育;较高温度(25℃)和较低盐度(10)处理对配子的放散有极显著影响,而较高温度(25℃)和较高盐度(40)处理对孢子的放散有极显著影响。配子体和孢子体的生殖发育对基本环境因子存在着一定的差异反应,但两者均可在较宽的温度和盐度范围内生殖,这可能是导致绿潮种群暴发式增长的主要原因之一。 相似文献
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蛋白质O-甘露糖基化是一种广泛存在于生物体内的蛋白质翻译后修饰过程,即在Dol-P甘露糖转移酶(PMT)催化下将长萜酰甘露糖上的甘露糖连接到肽链上丝氨酸或者苏氨酸羟基的过程。本文对国际千种植物转录组计划(1KP)中22种海洋红藻及19种海洋褐藻的转录组数据库进行搜索,获得了6条来自海萝(Gloiopeltis furcata)的PMT基因序列。通过对其序列特征的分析发现藻类PMT序列与真菌及动物具有相似的亲疏水性列谱,表明真核生物PMT间具有相似的跨膜区。藻类PMT的N端较为保守,但较其他真核生物PMT的loop5存在222个氨基酸的缺失,导致其loop5处缺少了3个MIR结构域使得loop5明显减小。此外,利用贝叶斯法构建系统进化树表明藻类的PMT基因来自于内共生过程的质体基因转移。 相似文献
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