Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress   总被引：4，自引：0，他引：4  

郭振宇焦传珍  相建海 《中国海洋湖沼学报》2004,22(4):386-391

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.  相似文献   

养殖期中国对虾抗菌肽的表达   总被引：4，自引：0，他引：4  

郭振宇  董波  焦传珍  相建海 《海洋科学》2004,28(1):48-51

利用半定量RT-PCR方法，根据中国对虾(Fenneropenaeus chinensis)抗菌肽的cDNA序列，设计一对特异引物，首次对中国对虾养殖期(PL11期仔虾～稚虾)抗菌肽的表达进行了研究。结果在各时期样本所提取的总RNA中，均扩增出一条长度约为200bp的特异性条带，表明中国对虾养殖期不同阶段均有抗菌肽的表达，提示中国对虾抗菌肽基因为组成型表达，并可能是中国对虾抵抗微生物感染的一个重要分子基础。  相似文献