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Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the eff ects of processing variables(extraction temperature, p H, extraction time, and liquid-tosolid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: p H 1.52, 63.62 min, 100°C and a liquid-to-solid ratio of 44.4 m L/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52±0.90% was obtained, which validated the model prediction. The eff ects of diff erent drying methods(freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural diff erences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by diff erent methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin(SDP) and apple pectin(AP) were stronger than those of vacuum oven dried pectin(ODP) and vacuum freeze dried pectin(FDP). Therefore compared with the other two drying methods, the spray drying method was the best.  相似文献   
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制备了羧甲基琼胶寡糖钒络合物,并利用ICP-MS测定钒的含量为4.6%;同时,对钒氧羧甲基琼胶寡糖抗氧化和抑制人肝癌BEL-7402细胞增殖活性进行研究。抗氧化实验结果表明,钒氧羧甲基琼胶寡糖清除?OH的能力和DPPH?的能力均比羧甲基琼胶寡糖的清除能力有显著提高。经磺酰罗丹明B蛋白染色法测试,进一步发现钒氧羧甲基琼胶寡糖在16~1 000 mg/L浓度范围内对人肝癌BEL-7402细胞的增殖有较好的抑制作用。  相似文献   
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采用红外光谱法对海藻多糖作为糖基配体与VO2+络合后形成的钒氧多糖络合物的结构进行了鉴定,并利用原子发射光谱测定了钒在络合物中的质量分数(0.11%),同时分析了钒氧多糖络合物对四氧嘧啶诱导的高血糖糖尿病小鼠的体质量、血糖水平以及糖耐量的影响。结果表明,钒氧多糖络合物对小鼠无急性毒性,小鼠对钒氧多糖的最大耐受量为2.4 g/kg,在糖尿病小鼠降血糖实验中高剂量组(0.5 g/kg)对空腹血糖和糖尿病小鼠糖耐受量有显著降糖作用。  相似文献   
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