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A full life-cycle toxicity test, combined with histology, on calanoid copepod Schmackeria poplesia was used to study the effect of bis(tributyltin) oxide (TBTO). The results indicate no sex-specific differences in TBTO toxicity. Long-term mortalities of the copepods exposed to concentrations higher than 20 ng TBTO L−1 were significantly elevated compared with that of control, and larval development was inhibited when they were exposed to 40 and 60 ng TBTO L−1. The percentages of ovigerous females were reduced compared with the control (P<0.01) after 24 days exposure to concentrations higher than 10 ng TBTO L−1. Histological examinations suggest that exposure to TBTO might block the posterior end of the diverticula and inhibits the production of egg sacs. A modified Euler-Lotka equation was used to calculate a population-level endpoint, the intrinsic rate of natural increase (r m), from individual life-table endpoints, i.e. mortality rate, time of release of first brood, sex ratio, the fraction of ovigerous females among all females as well as the number of nauplii per ovigerous female. Apart from the highest TBTO concentration (60 ng L−1), where all females aborted their egg sacs, 20 ng TBTO L−1 was the only concentration that significantly decreased r m compared with that of control (an effect associated with decreased sex ratio). The results show that the S. poplesia is affected by prolonged exposure to low concentrations of TBTO. The full life-cycle toxicity test combined with histology experiments provides more integral understanding of the toxicity of endocrine disrupters.  相似文献   
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A full life-cycle toxicity test,combined with histology,on calanoid copepod Schmackeria poplesia was used to study the effect of bis(tributyltin)oxide(TBTO).The results indicate no sex-specific differences in TBTO toxicity.Long-term mortalities of the copepods exposed to concentrations higher than 20 ng TBTO L-1were significantly elevated compared with that of control,and larval development was inhibited when they were exposed to 40 and 60 ng TBTO L-1.The percentages of ovigerous females were reduced compared with the control(P0.01)after 24 days exposure to concentrations higher than 10 ng TBTO L-1.Histological examinations suggest that exposure to TBTO might block the posterior end of the diverticula and inhibits the production of egg sacs.A modified Euler-Lotka equation was used to calculate a population-level endpoint,the intrinsic rate of natural increase(rm),from individual life-table endpoints,i.e.mortality rate,time of release of first brood,sex ratio,the fraction of ovigerous females among all females as well as the number of nauplii per ovigerous female.Apart from the highest TBTO concentration(60 ng L-1),where all females aborted their egg sacs,20 ng TBTO L-1was the only concentration that significantly decreased rm compared with that of control(an effect associated with decreased sex ratio).The results show that the S.poplesia is affected by prolonged exposure to low concentrations of TBTO.The full life-cycle toxicity test combined with histology experiments provides more integral understanding of the toxicity of endocrine disrupters.  相似文献   
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火腿许水蚤对牙鲆仔稚鱼成活、生长及脂肪酸组成的影响   总被引:1,自引:0,他引:1  
在分析火腿许水蚤、卤虫无节幼体及轮虫这3种饵料生物脂肪酸组成的基础上,研究它们对牙鲆仔稚鱼存活、生长及脂肪酸组成的影响。结果表明:火腿许水蚤体内3种高度不饱和脂肪酸(ARA,EPA,DHA)总量为34.18%,明显高于其它2种动物的含量(卤虫无节幼体4.67%;小球藻强化轮虫9.39%)。投喂火腿许水蚤的仔鱼成活率极显著高于投喂卤虫无节幼体的仔鱼,与投喂混合饵料(卤虫无节幼体和火腿许水蚤按比例混合)的仔鱼存活率差异较显著;其全长增长倍数和增重倍数极显著高于投喂卤虫无节幼体和混合饵料的仔鱼。投喂火腿许水蚤的稚鱼成活率显著高于投喂卤虫无节幼体的稚鱼,与投喂混合饵料的稚鱼存活率差异不显著;其全长增长倍数和增重倍数极显著高于投喂卤虫无节幼体和混合饵料的稚鱼。投火腿许水蚤的仔稚鱼体内ARA、EPA、DHA的含量明显高于投喂混合饵料的和投喂卤虫无节幼体的仔稚鱼。  相似文献   
4.
久效磷对单环刺螠体内几种酶活性影响的初步研究   总被引:1,自引:0,他引:1  
运用冰冻切片技术和酶组织化学的方法研究了长期暴露于不同质量浓度久效磷(0,50,100,150ng/L)下的单环刺螠(Urechisunicinctus)体内的乙酰胆碱酯酶(AChE)、细胞色素C氧化酶(CCO)、碱性磷酸酶(AKP)的活性变化。实验表明,AChE在单环刺螠的体壁上呈现出较强的酶活性,活性位点处为红棕色的颗粒沉淀、并随久效磷胁迫浓度的提高显色越来越深;消化道切片上基本上没有AChE的活性。CCO在单环刺螠的体壁及肠道上都呈现出较强的酶活性,活性位点显示为棕色;肠道上,不同质量浓度组由低到高其CCO活性位点依次增多、显色也依次加深。低质量浓度久效磷(<150ng/L)胁迫对单环刺螠体内的AKP活性没有明显的影响。  相似文献   
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