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Avianna F. Zhukovskaya Nina N. Belcheva Valentina S. Slobodskova Viktor P. Chelomin 《Ocean Science Journal》2012,47(3):189-195
Organisms have evolved a cellular response called stress protein response that increases their tolerance in adverse environmental conditions. Well known stress proteins that bind essential and toxic metals are metallothionein (MT). The scallop Mizuhopecten yessoensis is the most interesting organism because it is able to accumulate toxic cadmium in its digestive gland. However, in the tissue of the digestive gland of Mizuhopecten yessoensis MT (metallothioneins) have not been found. Eastern scallops, Mizuhopecten yessoensis, were collected from two locations ?? one clean and one polluted site. The concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were measured in the digestive gland. There was a significant increase in Cd concentrations in this studied tissue. We found that in the presence of cadmium Mizuhopecten yessoensis can induce high molecular proteins. The results of experiments have shown that Cd-binding ligands have a number of properties similar to MT: acetone and temperature stability; the ability to bind some metals, including Cd, Cu and Zn. Protein chromatography (FPLC, Superosa 12) from the digestive gland of scallop M. yessoensis has shown that cadmium is associated with high molecular weight Cd-binding proteins (72 kDa and 43 kDa). The major cadmium-binding protein 72 kDa is glycoprotein. In experiments we have demonstrated that Cd-binding proteins can be induced when there is cadmium exposure. The results of this study strongly suggest that the far eastern scallop Mizuhopecten yessoensis has a unique and well-developed system for the detoxification of heavy metals and it allows for biochemical systems to be maintained in a relatively stable manner in the presence of heavy metals. 相似文献
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Victor P. Chelomin Valentina V. Slobodskova Maksim Zakhartsev Sergey Kukla 《中国海洋大学学报(英文版)》2017,16(2):339-345
Copper oxide nanoparticles (CuO-NPs) are among the most widely used metal oxide nanoparticles, which increases the chance of their being released into the marine environment. As the applications of these particles have increased in recent years, their potential impact on the health of marine biota has also increased. However, the toxicological effects of these NPs in the marine environment are poorly known. In the present study, the DNA damaging potential of CuO-NPs in the marine eastern mussel Mytilus trossulus was evaluated and compared to that of dissolved copper exposures. Genotoxicity was assessed by the single cell gel electrophoresis (comet) assay in mussel gill and digestive gland cells. The results showed that copper in both forms (CuO-NPs and dissolved copper) was accumulated to different extents in mussel tissues. The mussel exposed to the dissolved copper attained higher concentrations of copper in the gills than in the digestive gland. In contrast to these results, it was found that CuO-NPs could induce much higher copper accumulation in the digestive gland than in the gills. A clear and statistically significant increase in DNA damage was found in both tissues of the Cu-exposed group compared to the control mussels. Our results indicated that the CuO-NP exposure produced remarkable effects and increased DNA damage significantly in mussel gill cells only. It should be noted that the digestive gland cells were prone to accumulation following CuO-NPs when compared to the gill cells, while the gill cells were more sensitive to the genotoxic effects of CuO-NPs. These results also suggested the need for a complete risk assessment of engineered particles before its arrival in the consumer market. 相似文献
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Belcheva NN Zakhartsev M Silina AV Slinko EN Chelomin VP 《Marine environmental research》2006,61(4):396-409
Seasonal and age-specific variations of cadmium (Cd) concentration in the digestive gland were investigated in the Japanese scallop Patinopecten yessoensis from Peter the Great Bay, Sea of Japan, with different degrees of Cd pollution. The seasonal changes in Cd concentrations of the digestive gland were inversely proportional to the dry weight of the gland. Concentrations of Cd and total Cd content (mug Cd per organ) increased with age (age-specific) to the same extent in contaminated and uncontaminated areas. There was also a strong positive correlation between Cd content in the whole digestive gland and shell weight and it is proposed that this relationship can be used as a new criterion for comparative evaluation of Cd levels in scallops from different areas We hypothesize that Cd is uptaken into scallops in proportion to the amount of calcium that absorbed through ion channels, and in addition, Cd in the digestive gland is in immobile forms (e.g. metal-rich granules) that accumulate with age. Moderate environmental pollution has no effect on the relationship between Cd content and shell size and the observed decrease in growth performance of the scallops from polluted areas may be due to other factors. 相似文献
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DNA damage in the gill cells of the marine scallop Mizuhopecten yessoensis during anoxic stress and aerobic recovery 总被引：2，自引：0，他引：2
Valentina V. Slobodskova Avianna F. Zhukovskaya Victor P. Chelomin 《Ocean Science Journal》2012,47(2):95-100
Anoxia-induced DNA damage in the gill cells of the marine scallop Mizuhopecten yessoensis was assessed with the alkaline comet assay (single-cell gel electrophoresis). The alkaline comet assay method for detecting DNA strand breaks and alkali labile sites in individual cells. DNA damage was determened in the scallops (M. yessoensis) gill cells. The scallops were exposed to air for 8 h showing a clear increase in the levels of DNA damage. After the air exposure, M. yessoensis were re-submersed for a period of 12 h, leading values to return to a pre-aerial exposure level. Control animals were kept immersed during the whole period. The resulting data demonstrate that natural influences, such as oxygen depletion (anoxia) in seawater, can be responsible for the induction of DNA damage. If the scallops were re-immersed in oxic conditions, the anoxically induced breaks were repaired. The main mechanisms influencing the integrity of the DNA structure are discussed in this paper. 相似文献
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Nina N. Belcheva Maxim V. Zakhartsev Nadezhda V. Dovzhenko Avianna F. Zhukovskaya Victor Ya. Kavun Victor P. Chelomin 《Ocean Science Journal》2011,46(2):85-94
The digestive gland and gills of the mussel Crenomytilus grayanus extracted from three locations — (i) sampled from a clean and (ii) polluted site and (iii) transplanted from the nonpolluted
to polluted site - were analysed for antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase), total oxyradical
scavenging capacity and levels of lipid peroxidation products (malondialdehyde, conjugated dienes and lipofuscin). Perturbation
of redox status was found in both digestive gland and gill tissues of mussels living in the polluted area. As the activities
of superoxide dismutase and catalase were 1.2–3 times higher, the total oxyradical scavenging capacity was lower by 20–35%
and the levels of lipid peroxidation products were 2–7 times higher compared to mussels from the reference site. In transplanted
mussels, the lipid peroxidation process in both tissues was significantly stimulated (the level of conjugated dienes was increased
1.7–2.5-fold; malondialdehyde and lipofuscin contents were increased 3.5–5-fold) and the total oxyradical scavenging capacity
fell by 50–70%. In addition, the transplantation generally resulted in transient and variable responses of antioxidant enzymes
for both tissues. Complex response-behaviour of the antioxidant enzymes strongly points to the necessity of employing a combined
approach that takes into account activities of antioxidant enzymes and the total oxyradical scavenging capacity, as well as
measurement of oxidative damage (e.g., lipid peroxidation) to evaluate the physiological health of molluscs. 相似文献
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