Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus.     

Suze Chora  Brian McDonagh  David Sheehan  Mireille Starita-Geribaldi  Michèle Roméo  Maria J Bebianno 《Marine environmental research》2008,66(1):95-97

Environmental pollutants, such as metals, are widespread in aquatic environments and can lead to the formation of reactive oxygen species (ROS). ROS are highly toxic in marine species since they can cause serious reversible and irreversible changes in proteins including ubiquitination and modifications such as carbonylation. This study aimed to confirm the potential of ubiquitination and carbonylation as markers of oxidative stress in the clam Ruditapes decussatus (Veneroida, Veneridae) exposed to cadmium (40 microg/L). After 21 days of exposure clams were dissected into gills and digestive gland. Cytosolic proteins of both tissues were separated by two-dimensional electrophoresis (2-D SDS-PAGE) and analysed by immunobloting. Higher ubiquitination and carbonylation levels were in digestive gland of contaminated organisms. These results confirm the potential of ubiquitination and carbonylation as a sensitive and specific marker of oxidative stress in marine bivalves. In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach.  相似文献   

Evaluation of approximate methods to estimate residual drift demands in steel framed buildings          下载免费PDF全文

Jorge Ruiz‐García  Carlos Chora 《地震工程与结构动力学》2015,44(15):2837-2854

This paper presents the evaluation of two approximate methods recently proposed in the literature to estimate residual (permanent) drift demands at the end of earthquake excitation for seismic assessment of buildings. Both methods require an estimate of the peak (maximum) interstory drift demand and the corresponding drift demand at significant yielding of the building. Additionally, an approximate method is proposed as part of this study. The introduced method follows a coefficient‐based approach similar to the Coefficient Method included in several US documents. For evaluating the approximate methods, five moment‐resisting steel framed buildings having different number of stories were analyzed under four sets of earthquake ground motions. Quantification of the accuracy of the approximate methods to estimate residual drift demands with respect to results from nonlinear time‐history analyses was performed through error measures computed for each building and each set of earthquake ground motions. Results show that the mean standard error tends to increase as the seismic hazard level increases. Between the two methods, the method introduced by Erochko et al. seems more effective in predicting residual drift demands than that proposed in the FEMA P‐58 recommendations in the USA. It is demonstrated that including additional sources of stiffness and strength in the modeling approach constrains the amplitude of residual drift demands. As a beneficial consequence, the accuracy of both approximate methods in predicting residual drift demands is significantly improved (i.e., mean standard error decreases). The introduced method also provides similar accuracy than the approximate methods available in the literature. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献