Purification of a Diatom and Its Identification to Cylindrotheca closterium     

WANG Song  ZHANG Lin  YANG Guanpin  ZHU Baohua  PAN Kehou 《中国海洋大学学报(英文版)》2015,(2):357-361

A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closterium according to its morphological characteristics and rbc L and 18 s r RNA gene sequences. The alga was not sensitive to ampicillin and neomycin, but sensitive to chloramphenicol which inhibited its growth at concentrations ranging from 50 to 150 μg m L-1. The purified alga was easy to culture and its specific growth rate was 0.207 ± 0.002(d-1). It was resistant to pollution and could be harvested in an easy way. It was relatively high in lipid content(20.08% ± 0.67% of dry weight) and the combined amount of its 16:0 and 16:1(n-7), the most suitable resource of biodiesel, was as high as 64% of the total fatty acids, while the amount of polyunsaturated fatty acids reached 19.96%–20% of the total fatty acids. Thus the purified C. closterium can be cultured as a biodiesel producer or a nutrition supplement producer.  相似文献   

Evaluation of Cytotoxicity and Genotoxicity of Insecticide Carbaryl to Flounder Gill Cells and Its Teratogenicity to Zebrafish Embryos     

EY Manish Raj  GUO Huarong 《中国海洋大学学报(英文版)》2015,(2):362-374

In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill(FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking(NRU), lactate dehydrogenase(LDH) releasing and Hoechst 33342 and propidium idodide(PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L-1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl(20 mg L-1, P 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L-1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time- and concentration-dependent manners at specific stages. The 24 h-, 48 h- and 96 h-LC50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L-1, respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC50 and LC50 indicated.  相似文献   

Influence of Uneven Trace Spacing on Rayleigh Wave Dispersion     

张双喜  李孟奎 《中国地质大学学报(英文版)》2011,(2)

Rayleigh wave dispersion signals are significant to underground investigation.Tradition-ally,uniformed trace spacing is employed in surface wave surveys.In some cases,however,uneven trace spacing is often encountered because of the limitations of the site condition.In order to study the influence of uneven trace spacing on the dispersion data construction of Rayleigh waves,data acquisi-tion is performed based on a 2.5D field layout with a linear array of geophones fixed and a mobile source.The observation d...  相似文献   

Anthropogenic Sulfate and Nitrate Signals in Snow from Bogda Glacier, Eastern Tianshan     

张晓宇  Ross Edwards 《中国地质大学学报(英文版)》2011,(4)

A 1.2 m snow pit was recovered on July 29th, 2009 from the Bogda Glacier, eastern Tianshan (天山). The sample site temperature of -9.6 ℃ indicates that the unique glaciochemical re-cord was well preserved and suitable for the reconstruction of air pollution levels in this previously un-explored region. Samples were analyzed for major ions (Na+, K+, Ca2+, Mg2+, NH4+, Cl-, SO42-, NO3-, HCOO-, and CH3COO-). NO3- and SO42- were characterized by significant high levels of pollution con-centration. Most air masses ...  相似文献   

Monitoring Glacial Shrinkage Using Remote Sensing and Site-Observation Method on Southern Slope of Kalik Mountain, Eastern Tian Shan, China     

王文彬  李开明  高建芳 《中国地质大学学报(英文版)》2011,(4)

Currently, one of the effective means in monitoring glacier change in regional scale is remote sensing and site-observation method. In this article, we present a study of comparing glacier area in 2005 derived from SPOT5 satellite image with area in 1972 derived from topographic maps. Moreover, Miaoergou (庙儿沟) flat-summit glacier is site observed to verify glacial change in regional scale. During the study period, glaciers located in the southern slope of Kalik (喀尔里克) Mountain re-duced their area by 12.3%. ...  相似文献   

Deposition Process of Dust Microparticles from Aerosol to Snow-Firn Pack on Glacier No. 1 in Eastern Tianshan Mountains, China     

尤晓妮  董志文 《中国地质大学学报(英文版)》2011,(4)

Samples were continuously collected from aerosol, fresh snow, and snow pits on Glacier No. 1 at Urumqi River source in eastern Tianshan (天山) Mountains. The deposition processes and the characteristics of mineral dust microparticles from aerosol to fresh snow, and then evolution to the snow pit were determined. Total dust microparticle concentration in the surface snow and aerosol showed a similar temporal variation trend, which was strongly associated with regional and local at-mospheric circulation in the ...  相似文献   

Comparison of Results of Recent Seismic Profiles with Tectonic Models of the North China Craton     

Timothy M Kusky 《中国地质大学学报(英文版)》2011,(2)

The geometry and timing of amalgamation of the North China craton(NCC) have been controversial,with three main models with significantly different interpretations of regional structure,geochronology,and geological relationships.The model of Zhao G C et al.suggests that the eastern and western blocks of the NCC formed separately in the Archean,and an active margin was developed on the eastern block between 2.5 and 1.85 Ga,when the two blocks collided above an east dipping subduc-tion zone.The model of Kusky ...  相似文献   

Postrift Rapid Subsidence Characters in Qiongdongnan Basin,South China Sea     

尹新义  任建业  雷超  王珊  张俊霞 《中国地质大学学报(英文版)》2011,(2)

In this article,the backstripping technique was used in studying the subsidence charac-ters of the Qiongdongnan(琼东南) basin(QDNB) in order to understand its dynamic mechanism of formation and evolution.Meanwhile,the geothermal characteristics of this area were summarized,and the stretching factors(β) of the upper crust,the whole crust,and the whole lithosphere were calculated.The QDNB is characterized by high subsidence rate,high geothermal gradient,high geothermal heat flow,and the lithosphere stretching an...  相似文献   

Tectonics and Mechanisms of Uplift in the Central Uplift Belt of the Huimin Depression     

倪金龙  郭颖  王智敏  刘俊来  林玉祥  李月 《中国地质大学学报(英文版)》2011,(3)

The Huimin (惠民) depression is a third-level tectonic element of the Bohai (渤海) Bay basin in eastern China. The central uplift belt is the most important oil and gas accumulation zone in the depression, but the lack of adequate geological studies in the area has greatly hindered exploration and development. In this article, using seismic data, fracture mechanics, and a combination of data on fault growth indices and fault throws, we present an analysis of tectonic activity in the central uplift belt and adja...  相似文献   

秦南地区天然气成因与油气勘探潜力分析     

庄新兵  邹华耀  李楠  魏刚  孙和风 《吉林大学学报(地球科学版)》2011,41(3):680-688

为了评价和认识秦南地区有重大勘探突破的陡坡带和该区的勘探潜力,利用该地区天然气组分和碳同位素等分析资料、伴生原油的油源对比以及油气运移模拟,定量刻画与定性评价相结合,对秦南地区天然气成因和油气勘探潜力进行了系统研究.研究表明:凸起带天然气为新近系溶解气且以干气为主,陡坡带天然气为古近系凝析气且为湿气;陡坡带天然气主要为...  相似文献