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The aim of the present study was to investigate the protein-sparing effect of carbohydrate in diets for juvenile turbot(Scophthalmus maximus) reared at five salinities(12,18,24,30,and 36).The fish were fed three isocaloric and isolipidic diets for 60 days.The results show that specific growth rate(SGR)and feed conversion efficiency(FCE) were higher in fish reared at salinities of 18 and 36,but lower at 12.Fish fed with diet C25P40(25%carbohydrate and 40%protein) had lower SGR and FCE values compared with those fed with the C5P52(5%carbohydrate and 52%protein) and C15P46(15%carbohydrate and 46%protein) diets;however,there was no statistical difference between diet C5P52 and C15P46.SGR and FCE values were unaffected by diet composition in fish reared at salinity 36.Hepatic lipogenic enzyme activities were higher in fish reared at 18 and 36,but lower at 12,while glucokinase(GK) activity was higher in fish reared at 12,and lower at 18 and 36.Dietary starch enhanced GK activity while depressing lipogenic enzyme activity.However,lipogenic enzyme activity increased with increasing dietary starch in fish reared at 36.It is recommended that salinity should be maintained 12 in the farming of juvenile turbot.In addition,an increase in gelatinized starch from 5%to 15%could spare 6%dietary protein in fish reared at salinities of 18-30,while higher salinity(36) could improve dietary carbohydrate use and enhance the protein-sparing effect,which is linked with the induction of lipogenic capacities. 相似文献
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为了探讨盐度对幼鱼肌肉中氨基酸和脂肪酸组成的影响,作者将平均体质量为(7.16±0.07)g的大菱鲆(Scophthal musmaximus)幼鱼分别饲养在盐度(12、18、24、30(对照组)和36)水体中60 d。结果表明,不同盐度下,幼鱼肌肉中的氨基酸总量(TAA)、必需氨基酸(EAA)总量、非必需氨基(NEAA)总量和呈味氨基酸(FAA)总量分别在69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%和24.20%±0.66%~26.98%±1.02%,从高到低依次为36组30组12组18组24组,36组均显著高于24组(P0.05)。支链氨基酸(BCAA)总量、EAA/TAA、EAA/NEAA、FAA/TAA分别在14.94%±0.34%~15.37%±0.18%、44.57%±1.20%~45.78%±0.26%、80.40%±3.93%~84.42%±0.89%和34.95%±0.70%~36.41%±1.33%,各盐度组间均无显著性差异(P0.05)。必需氨基酸指数(EAAI)在40.41±1.23~44.46±0.54,30和36组显著大于其他组(P0.05)。脂肪酸(TFA)总量和非必需脂肪酸(NEFA)总量分别在88.65%±0.68%~89.66%±0.32%和85.11%±0.78%~86.38%±0.37%,12和30组均显著高于18组(P0.05)。多不饱和脂肪酸(PUFAS)总量和EPA+DHA总量分别在34.95%±1.01%~36.88%±0.64%和23.53%±1.30%~25.74%±0.25%,从高到低依次为36组30组24组12组18组,各盐度组间均无显著性差异(P0.05)。必需脂肪酸(EFA)总量在3.28%±0.04%~3.58%±0.10%,各盐度组间均无显著性差异(P0.05)。饱和脂肪酸(SFA)总量在27.51%±0.37%~29.83%±0.35%,12组显著高于36组(P0.05)。与对照组相比,36组大菱鲆幼鱼肌肉中的氨基酸和脂肪酸组成有所改善,其蛋白质和脂肪质量得到提高。研究成果可为大菱鲆渗透压调节的能量代谢机制及肌肉营养品质的环境调节机理积累基础资料。 相似文献
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