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土地利用变化受到地形地貌、自然环境、城市规划和经济发展等的影响,预测其未来情景对政策调整具有重要的参考意义。元胞自动机模型是模拟和预测不同规划政策下土地利用变化的常用方法。本文基于GlobeLand30数据集,利用浙中城市群2000-2010年土地利用变化校准FLUS模型,并模拟2010年土地利用格局,其总体精度、Kappa系数和图形优化(FOM)分别为89.74%、82.69%和29.86%。采用马尔可夫链预测2030年各类型土地总量,利用FLUS预测一般条件下(常规情景)和城市轨道交通规划站点影响下(轨交情景)浙中城市群未来土地格局。结果表明,在5 km范围内城市轨道交通站点对建设用地增长影响较大,在该区域轨交情景比常规情景面积增加45.25 km^2、且主要发生在城市边缘区。建设用地扩张主要通过侵占优质农田实现,轨交情景5 km范围内农田转化为建设用地比常规情景增加33.34 km^2,建设用地扩张强度高于常规情景,其中最低扩张强度以上占比高于常规情景3.70%。景观指数表明,2种情景中林地、草地和水域格局具有较高相似性。本研究表明,综合使用FLUS、遥感、GIS等技术方法,能够准确模拟和预测不同规划条件下未来土地利用格局,并为规划和政策调整提供高可信空间数据。 相似文献
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Microsatellites are a ubiquitous component of the eukaryote genome and constitute one of the most popular sources of molecular markers for genetic studies. However, no data are currently available regarding microsatellites across the entire genome in oysters, despite their importance to the aquaculture industry. We present the fi rst genome-wide investigation of microsatellites in the Pacifi c oyster Crassostrea gigas by analysis of the complete genome, resequencing, and expression data. The Pacifi c oyster genome is rich in microsatellites. A total of 604 653 repeats were identifi ed, in average of one locus per 815 base pairs(bp). A total of 12 836 genes had coding repeats, and 7 332 were expressed normally, including genes with a wide range of molecular functions. Compared with 20 different species of animals, microsatellites in the oyster genome typically exhibited 1) an intermediate overall frequency; 2) relatively uniform contents of(A)n and(C)n repeats and abundant long(C)n repeats(≥24 bp); 3) large average length of(AG)n repeats; and 4) scarcity of trinucleotide repeats. The microsatellite-fl anking regions exhibited a high degree of polymorphism with a heterozygosity rate of around 2.0%, but there was no correlation between heterozygosity and microsatellite abundance. A total of 19 462 polymorphic microsatellites were discovered, and dinucleotide repeats were the most active, with over 26% of loci found to harbor allelic variations. In all, 7 451 loci with high potential for marker development were identifi ed. Better knowledge of the microsatellites in the oyster genome will provide information for the future design of a wide range of molecular markers and contribute to further advancements in the fi eld of oyster genetics, particularly for molecular-based selection and breeding. 相似文献
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A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis 总被引：2，自引：0，他引：2
The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I （COI） of five common Crassostrea species in China （including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas） and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers （Oyster- COI-1）, we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species. 相似文献
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