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1.
粘孢子虫病（myxosporadisis）是一种鱼类常见的侵袭性疾病，在所调查过的鱼类中，几乎每一种鱼都可感染，有时同一种鱼可感染多种粘孢子虫，甚至在同一条鱼的同一器官或组织内都有可能存在几种粘孢子虫。粘孢子虫种类繁多，分布广泛，鱼类感染早期难以预防，感染后难以治疗。目前，渔业生产中对于粘孢子虫病的诊断主要依赖于镜检粘孢子虫的结构， 相似文献
2.
聂品 《中国海洋湖沼学报》2000,(1)
ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｔｅｓｔｉｎａｌｐａｒａｓｉｔｅｃｏｍｍｕｎｉｔｉｅｓｉｎｆｉｓｈａｒｅｆｏｕｎｄｎｏｒｍａｌｌｙｔｏｂｅｐｏｏｒｉｎｔｅｒｍｓｏｆｓｐｅｃｉｅｓｃｏｍｐｏ ｓｉｔｉｏｎｓｗｈｅｎｃｏｍｐａｒｅｄｗｉｔｈｔｈｏｓｅｉｎｂｉｒｄｓａｎｄｓｏｍｅｏｔｈｅｒｈｉｇｈｅｒｖｅｒｔｅｂｒａｔｅｓ (Ｋｅｎｎｅｄｙｅｔａｌ.,1 986;Ｅｓｃｈｅｔａｌ.,1 990 ) .Ｈｏｗｅｖｅｒ,ｅｃｔｏｐａｒａｓｉｔｅｃｏｍｐｏｓｉｔｉｏｎｓｏｎｇｉｌｌｓｏｆｆｉｓｈａｒｅｃｏｎｓｉｄｅｒｅｄａｓｓｐｅｃ… 相似文献
3.
为了研究大黄鱼（Pseudosciana crocea）peroxiredoxin Ⅳ（Lyc-Prx Ⅳ）在细胞内的抗氧化功能,构建了表达大黄鱼Prx Ⅳ的重组质粒p CMV-Lyc-Prx Ⅳ,瞬时转染人胚肾细胞（HEK-293T）,利用Western blotting方法检测转染后的细胞样品中Lyc-Prx Ⅳ的表达情况,并通过测定细胞内过氧化氢浓度来评价Lyc-Prx IV的体内抗氧化作用.结果显示,Lyc-Prx Ⅳ可在转染重组质粒p CMV-Lyc-Prx Ⅳ的HEK-293T细胞中表达,且细胞中A560处的吸光值在转染后6、12、24 h后分别为0.154、0.116以及0.162,而瞬时转染空载体p CMV后细胞样品在A560处的吸光值在转染后一直稳定在0.260左右,说明细胞中过氧化氢的浓度在转染后6、12、24 h时明显低于瞬时转染空载体p CMV细胞中的过氧化氢浓度（p〈0.01）.表明Lyc-Prx Ⅳ在生物体内可以分解过氧化氢,参与生物体内氧化还原状况的调控. 相似文献
4.
The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3γ/δ and CD3ε contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3γ/δ and CD3ε showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3γ/δ. Additionally, while an N-glycosylation site was present in CD3ε, the site was not observed in CD3γ/δ. The CD3γ/δ and CD3ε subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3γ/δ and CD3ε in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3γ/δ and CD3ε were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3γ/δ and CD3ε were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3γ/δ and CD3ε mRNA, indicating that CD3γ/δ and CD3ε lymphocytes are involved in the immune response of this species. 相似文献
5.
为探索改革开放以来长株潭城市群的土地利用变化特征,本文首先对1978、1994、2006年的卫星遥感数据采用决策树和智能分类相结合的方法分类,1990、2000、2010年的土地利用数据和2015年地理国情普查数据重分类.通过统计计算,定量分析长株潭主城区各时相的土地利用综合程度、土地利用程度变化量和变化率,通过空间叠加,定性分析长株潭主城区自改革开放以来土地利用相互转换程度及各转换类型所在区域.研究结果表明,耕地、林地和建筑用地所占比例变化较大,水域、草地、未利用地所占比例较小,土地利用一直处于发展状态,每年耕地和林地大幅度向建筑用地转换,不同时相转换区域各有差异.本文研究可为长株潭城市群土地利用规划提供参考,且有利于长株潭主城区土地利用的可持续发展和生态环境保护. 相似文献
6.
SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY 总被引：2，自引：0，他引：2
Serum immunoglobulin from the mandarin fish,or the so-called Chinese perch,Siniperca chuatsi(Basilewsky),was successfully purified using affinity chromatography.Heavy and light chains were detected on electrophoresis gel,with molecular weights being estimated at 72 and 29 kDa,respectively.The tetrameric IgM of S.chuatsi was calculated to be 808 kDa.The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Westem blot analysis.The antisera reacted strongly with the heavy chains of S.chuatsi immunoglobulin.Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody. 相似文献
7.
ＩＮＴＲＯＤＵＣＴＩＯＮＭｙｘｏｓｐｏｒｅａｎｓａｒｅｖｅｒｙｃｏｍｍｏｎｐａｒａｓｉｔｅｓｏｆｆｉｓｈ .Ｔｈｅｉｒｓｅａｓｏｎａｌｆｌｕｃｔｕａｔｉｏｎｓｉｎｐｒｅｖａｌｅｎｃｅａｎｄｉｎｔｅｎｓｉｔｙｏｆｉｎｆｅｃｔｉｏｎｗｅｒｅｄｅｓｃｒｉｂｅｄｂｙｓｏｍｅａｕｔｈｏｒｓ (Ｍｉｔｃｈｅｌｌ,1 989;Ｓｉｔｊａ ＢｏｂａｄｉｌｌａａｎｄＡｌｖａｒｅｚ Ｐｅｌｌｉｔｅｒｏ,1 990 ;Ｃｏｎｅ ,1 994 ) .Ｈｏｗｅｖｅｒ,ａｌｍｏｓｔｎｏｒｅｌａｔｅｄｉｎｆｏｒｍａｔｉｏｎｉｓａｖａｉｌａｂｌｅｉｎＣｈｉｎ… 相似文献
8.
聂品 《中国海洋湖沼学报》1997,(1)
mODUcrIONDuringaninvestigationofthehtrithcorTunnitiesofFishinthehighlandlakesinYunnanProvinceofChina,aastodewasfoundintheintchtinesofafish,SinocycIocheiIusgrahamitingiFangwhichoccursonlyinFuxianLake(Chuetal.,l989),oneofthesevenlakesstudied.ThecharaCteristiesoftheastodespdriobtainedassignsthemdearlytothegenusProt~alus.FurtherexathenationstalustoconsiderthisastodeasanewspedesinthegenusProteOCotalus.Thisnewspedisdescritalinthispaper.MareRIusroMErnOosFuxianLake,theserenddeepestlakeinC… 相似文献
9.
Pansharpening方法通过融合多光谱影像的光谱信息和全色影像的空间细节信息来得到高分辨多光谱影像。然而传统的Pansharpening方法易导致产生光谱扭曲和空间信息丢失现象。受到影像稀疏表示超分重建理论启发,本文提出了一种新的基于稀疏表示和字典学习的Pansharpening方法。该方法以影像的高频特征作为训练样本,通过字典学习的方法来获取高低分辨率影像字典,使用正交匹配追踪算法求解出影像的稀疏表示系数,最终通过高分辨影像字典与稀疏系数相乘得到融合影像。实验结果表明:本文提出的方法能很好地保持遥感影像的光谱信息和空间细节信息。 相似文献
10.
Flavobacterium columnare causes columnaris disease in freshwater fish. In the present study, the antigenic regions of five outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purified recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella. Following vaccination of the fish their IgM antibody levels were examined, as was the expression of IgM, IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC IIβ, which are also involved in adaptive immunity. Interleukin genes (IL), including IL-1β, IL-8 and IL-10, and type I and type II interferon (IFN) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), significant increases in IgM antibody levels were observed in the fish vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM, IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fish. At four wpv, the fish were challenged with F. columnare, and the vaccinated fish showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the five OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fish and protection against F. columnare. 相似文献