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Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics, alone or in combination, are often used to avoid bacterial contamination during microalgal isolation and culture. In our preliminary trials, we found that many microalgae ceased growing in antibiotics at extremely high concentrations but could resume growth quickly when returned to an antibiotics-free liquid medium and formed colonies when spread on a solid medium. We developed a simple and highly efficient method of obtaining axenic microalgal cultures based on this observation. First, microalgal strains of different species or strains were treated with a mixture of ampicillin, gentamycin sulfate, kanamycin, neomycin and streptomycin (each at a concentration of 600 mg/L) for 3 days; they were then transferred to antibiotics-free medium for 5 days; and finally they were spread on solid f/2 media to allow algal colonies to form. With this method, five strains of Nannochloropsis sp. (Eustigmatophyceae), two strains of Cylindrotheca sp. (Bacillariophyceae), two strains of Tetraselmis sp. (Chlorodendrophyceae) and one strain of Amphikrikos sp. (Trebouxiophyceae) were purified successfully. The method shows promise for batch-purifying microalgal cultures. 相似文献
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基因工程在提高微藻生产生物柴油能力中的应用前景 总被引：1，自引：0，他引：1
石油消耗,气候变化,能源安全等问题的日益突出,使得可再生能源研究逐渐受到世界各地的关注。微藻富含油脂,具有很多独特的优势,在生物柴油领域被寄予厚望,但利用微藻生产生物柴油也有许多问题亟待解决。通过基因工程的手段对微藻进行改造有可能是解决这一问题的突破点。国内外通过基因工程手段在高等植物、微藻、微生物中均实现过提高油脂含量或改变油脂组成的目的,而这些经验能够为基因工程在微藻中的应用起到很好的指导作用。对此,本文作了比较详细的介绍以期为利用微藻生产生物柴油提供些有价值的信息。 相似文献
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