采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。 相似文献
Results obtained from simulating the propagation of infrasonic waves from the Chelyabinsk meteoroid explosion observed on February 15, 2013, are given. The pseudodifferential parabolic equation (PDPE) method has been used for calculations. Data on infrasonic waves recorded at the IS31 station (Aktyubinsk, Kazakhstan), located 542.7 km from the likely location of the explosion, have been analyzed. Six infrasonic arrivals (isolated clearly defined pulse signals) were recorded. It is shown that the first “fast” arrival (F) corresponds to the propagation of infrasound in a surface acoustic waveguide. The rest of the arrivals (T1–T5) are thermospheric. The agreement between the results of calculations based on the PDPE method and experimental data is satisfactory. The energy E of the explosion has been estimated using two methods. One of these methods is based on the law of conservation of the acoustic pulse I, which is a product of the wave profile area S/2 of the signal under analysis and the distance to its source EI [kt] = 1.38 × 10–10 (I [kg/s])1.482. The other method is based on the relation between the energy of explosion and the dominant period T of recorded signal ET [kt] = 1.02 × (T [s]2/σ)3/2, where σ is the dimensionless distance determining the degree of nonlinear effects during the propagation of sound along ray trajectories. According to the data, the explosion energy EI,T ranges from 1.87 to 32 kt TNT. 相似文献
滨海湿地作为人类活动和全球变化反应最为敏感的区域，其沉积记录可以反映出周边地区环境变化及人类活动信息。珠江口淇澳岛滨海湿地钻孔分析结果表明，在中全新世期间淇澳岛附近海域为河口湾环境，在风化层以上开始出现淤积，但在4 200 a BP前后受极冷气候的影响，沉积物粗化；自2 500 a BP以来，沉积环境相对稳定，在小冰期期间略有变化。沉积速率计算结果显示:淇澳岛附近海域自中全新世高海面以来的平均沉积速率为0.29 cm/a，4 160~2 500 a BP、2 500 a BP-1488年、1488-1893年、1893-1986年、1990-2007年期间的平均沉积速率分别为:0.17 cm/a、0.23 cm/a、0.35 cm/a、1.37 cm/a和5.94 cm/a，沉积速率逐渐增大，反映了珠江三角洲演化过程中沉积相与沉积环境的变化；1986-1990年期间的海堤建造极大地扰动了该钻孔上部的沉积过程，在工程施工期间共沉积了厚度约112 cm的沉积层，而在海堤建成后，沉积速率也显著增大。沉积物总有机碳、总氮和C/N值的垂向分布表明，在4 160~2 500 a BP期间受海洋环境影响较大，沉积物中有机碳以海源为主，2 500 a BP以来沉积物中碳、氮含量明显增大，C/N也相应变大，有机碳主要来源于陆源输入，但在小冰期期间海源有机碳贡献略有所增大；近百年来由于受人类活动影响显著，陆源有机碳的贡献快速增加。 相似文献
Atlantic salmon reared in recirculating aquaculture system (RAS) may lead to inappropriately high stocking density, because fish live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the influence of stocking density on growth and some stress related physiological factors were investigated to evaluate the effects of stocking density. The fish were reared for 220 days at five densities (A: 24 kg/m3; B: 21 kg/m3; C: 15 kg/m3; D: 9 kg/ m3 and E: 6 kg/m3 ). The results show that 30 kg/m3 might be the maximum density which RAS can afford in China. The stocking densities under 30 kg/m3 have no effect on mortality of Atlantic salmon reared in RAS. However, the specific growth rate (SGR), final weight and weight gain in the high density group were significantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate (FCR) had a negative correlation with density. Plasma hormone T3 and GH showed significant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone (T3), GH (growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT (glutamic oxalacetic transaminase) and GPT (glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no effects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise efficient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.
