排序方式： 共有25条查询结果，搜索用时 15 毫秒
1.
Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94%–100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments. 相似文献
2.
Takushi Niki Miwa Shimizu Ayako Fujishiro Junji Kinoshita 《Journal of Oceanography》2007,63(5):873-877
During time-series observations in Sagami Bay, Japan, the concentration of dissolved dimethylsulfoniopropionate (DMSPd), a precursor of dimethylsulfide (DMS), was negatively correlated with salinity. In the laboratory, low-salinity shock reduced
DMS production rates of the natural bacterial community and induced rapid DMSP release from a dinophyte, Heterocapsa triquetra, suggesting that low-salinity shock reduced DMSPd consumption but enhanced DMSPd production, which agrees with the negative correlation between DMSPd and salinity observed in Sagami bay. In addition, low-salinity shock did not affect DMSP lyase activity of H. triquetra. Low-salinity shock would increase the contribution from algae in DMS production, leading to an increase in potential DMS
productivity in the environment. 相似文献
3.
4.
The presence of internal variability (IV) in ensembles of nested regional climate model (RCM) simulations is now widely acknowledged in the community working on dynamical downscaling. IV is defined as the inter-member spread between members in an ensemble of simulations performed by a given RCM driven by identical lateral boundary conditions (LBC), where different members are being initialised at different times. The physical mechanisms responsible for the time variations and structure of such IV have only recently begun to receive attention. Recent studies have shown empirical evidence of a close parallel between the energy conversions associated with the time fluctuations of IV in ensemble simulations of RCM and the energy conversions taking place in weather systems. Inspired by the classical work on global energetics of weather systems, we sought a formulation of an energy cycle for IV that would be applicable for limited-area domain. We develop here a novel formalism based on local energetics that can be applied to further our understanding IV. Prognostic equations for ensemble-mean kinetic energy and available enthalpy are decomposed into contributions due to ensemble-mean variables (EM) and those due to deviations from the ensemble mean (IV). Together these equations constitute an energy cycle for IV in ensemble simulations of RCM. Although the energy cycle for IV was developed in a context entirely different from that of energetics of weather systems, the exchange terms between the various reservoirs have a rather similar mathematical form, which facilitates some interpretations of their physical meaning. 相似文献
5.
The geochemistry of Boron-rich groundwater of the Karlovassi Basin, Samos Island, Greece 总被引：1，自引：0，他引：1
Michael G. Stamatakis Evangelos P. Tziritis Niki Evelpidou 《Central European Journal of Geosciences》2009,1(2):207-218
The upper Miocene of Karlovassi Basin, Samos Island, Greece, contain continental evaporites such as colemanite, ulexite, celestite, gypsum and thenardite. These evaporites are related with volcanic tuffs, diagenetically altered in a saline-alkaline lake environment. The aim of the present paper is to: a) define the impact of the already known and possible buried borates and other evaporites to the geochemistry of the hydrogeological system of Karlovassi Basin, and; b) to assess the correlation between surface and underground evaporite deposits considering the spatial changes in the concentrations of the examined physicochemical parameters. Fieldwork, laboratory measurements and literature data revealed elevated boron values (2136–33012 ?/L) in the central part of Karlovassi Basin. In the same area, high amounts of strontium, sodium, lithium and sulfates also occur. It is proposed that these ions originate from the leaching of evaporites and authigenic minerals such as the Sr-rich clinoptilolite and the boron-bearing potassium feldspar. Boron values are abnormally high for freshwater aquifers, and are indicative of the presence of buried evaporites in the basin with unknown significance. 相似文献
6.
An Application of Hydraulic Tomography to a Large‐Scale Fractured Granite Site,Mizunami, Japan 
下载免费PDF全文
下载免费PDF全文 Yuanyuan Zha Tian‐Chyi J. Yeh Walter A. Illman Tatsuya Tanaka Patrick Bruines Hironori Onoe Hiromitsu Saegusa Deqiang Mao Shinji Takeuchi Jet‐Chau Wen 《Ground water》2016,54(6):793-804
While hydraulic tomography (HT) is a mature aquifer characterization technology, its applications to characterize hydrogeology of kilometer‐scale fault and fracture zones are rare. This paper sequentially analyzes datasets from two new pumping tests as well as those from two previous pumping tests analyzed by Illman et al. (2009) at a fractured granite site in Mizunami, Japan. Results of this analysis show that datasets from two previous pumping tests at one side of a fault zone as used in the previous study led to inaccurate mapping of fracture and fault zones. Inclusion of the datasets from the two new pumping tests (one of which was conducted on the other side of the fault) yields locations of the fault zone consistent with those based on geological mapping. The new datasets also produce a detailed image of the irregular fault zone, which is not available from geological investigation alone and the previous study. As a result, we conclude that if prior knowledge about geological structures at a field site is considered during the design of HT surveys, valuable non‐redundant datasets about the fracture and fault zones can be collected. Only with these non‐redundant data sets, can HT then be a viable and robust tool for delineating fracture and fault distributions over kilometer scales, even when only a limited number of boreholes are available. In essence, this paper proves that HT is a new tool for geologists, geophysicists, and engineers for mapping large‐scale fracture and fault zone distributions. 相似文献
7.
This study aims to investigate the spatiotemporal trends in snow depth (SD) and snow cover extent (SCE) for Arctic lands, excluding Greenland, for the period 1948–2006. The investigation not only delineates how the Arctic regions are manifesting significant annual trends in both SD and SCE, but also provides a comprehensive understanding of their historical context. To achieve these objectives, the combined resources of the hydrological and biogeochemical model (CHANGE), National Oceanic and Atmospheric Administration (NOAA) weekly SCE data, and in situ observations of SD were used. Most regions in the Arctic exhibited a significant negative trend in SD over the 59 years of study. The magnitude of the negative trend was stronger in North America than in Eurasia, where the decrease became more significant, starting from the late 1980s, coinciding well with the temperature rise during that time. During the same period, the warming temperature caused a prominent decrease in deeper SDs (i.e., >35 cm), so that the corresponding SCEs exhibited negative anomalies, with the greatest declines being observed at SDs > 55 cm. In contrast, the SCEs for SD ≤ 35 cm showed increased anomalies during the most recent two decades. The increased anomalies signify a sequential result induced by the decrease in the SCEs with deeper SDs, rather than the expansion of snow to snow-free regions. These changes resulted in a northward shift of the shallow SD line, which took place to a highly significant degree in North America. These results suggest that the Arctic SCE and SD will undergo more intense changes in response to the future climate warming. 相似文献
8.
Mohammad Basyuni Hirosuke Oku Shigeyuki Baba Kensaku Takara Hironori Iwasaki 《Journal of Oceanography》2007,63(4):601-608
Isoprenoids in the nonsaponifiable lipids (NSL) fraction of Okinawan mangroves were analyzed with special reference to their
importance as lipid input into estuarine ecosystems. Triterpenoids and phytosterols comprised the major proportion of NSL.
Identifications of the triterpenoids and phytosterols in NSL fraction were based on the interpretation of 13C-NMR and GC-MS spectra. The triterpenoids and phytosterols mainly comprised 11 and 6 compounds, respectively. The major components
were lupeol, β-amyrin and taraxerol for triterpenoids, and β-sitosterol and stigmasterol for phytosterols. The diversity in
the terpenoid composition was noted in both leaves and roots of mangrove species, implying the chemotaxonomic utility of terpenoids.
Terpenoid compositions of the root are not always similar to that of leaf, suggesting that terpenoids in the root are produced
by biosynthesis in situ, not by translocation of the synthate from the leaf. Terpenoids existed in greater proportion in the
outer parts of the root. These data are likely to contribute to estimating the lipid input from mangrove trees adjacent to
estuarine sediments and the ocean. 相似文献
9.
Takamitsu Yamanaka Yutaka Komatsu Hironori Nomori 《Physics and Chemistry of Minerals》2007,34(5):307-318
Many of ilmenites ABO3 compounds bearing transition elements have semiconductive, ferroelectric and antiferromagnetic properties. The high-pressure
diffraction studies of FeTiO3 have been conducted up to 8.2 GPa using synchrotron radiation in KEK at Tsukuba with diamond anvil cell. The compression
mechanism of FeTiO3 ilmenite has been investigated by the structure refinements converged to the reliable factors R = 0.05. The deformations of the FeO6 and TiO6 octahedra were reduced with increasing pressure. In order to elucidate the electric conductivity change with pressure, electron
density distribution of ilmenite have been executed by maximum entropy method (MEM) using single-crystal diffraction intensity
data. MEM based on F
obs(hkl) of FeTiO3 clearly shows electron density in comparison with the difference Fourier synthesis based on F
obs(hkl) − F
calc(hkl). The radial distribution of the electron density indicates electron localization around the cation positions. The bonding
electron density found in bond Fe–O and Ti–O is lowered with pressure. The isotropic temperature factors B
iso become smaller with increasing pressure. Nevertheless the thermal vibration is considerably restrained by the compression,
the electric conductivity is enhanced with pressure. Neither charge transfer nor electron hopping between Fe and Ti along
the c axis in FeTiO3 is plausible under high pressure. But the electric conductivity due to electron super-exchange in Fe–Fe and Ti–Ti has been
clarified by the MEM electron density distribution. The anisotropy in the electric conductivity has been clarified. 相似文献
10.
Dynamic effects of moving loads on road pavements: A review 总被引：3，自引：0，他引：3
This review paper deals with the dynamic response of road pavements to moving loads on their surface. The road pavement can be modeled as a beam, a plate, or the top layer of a layered soil medium. The foundation soil can be modeled as a system of elastic springs and dashpots or a homogeneous or layered half-space. The material behavior of the pavement can be elastic or viscoelastic, while that of the foundation layers elastic, viscoelastic, water-saturated poroelastic or even inelastic. The loads are concentrated or distributed of finite extent, may vary with time and move with constant or variable speed. The analysis is done by analytical, analytical/numerical and purely numerical methods, such as finite element and boundary element methods, under conditions of plane strain or full three-dimensionality. A number of representative examples is presented in order to illustrate the problem and the methods of analysis, demonstrate the dynamic effects of moving loads on the layered soil medium and indicate the implications of the results on road and airport pavement design. 相似文献