The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)     

Xabier Arzuaga  Wanda Calcao  Adria Elskus 《Marine environmental research》2004,58(2-5):517

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.  相似文献   

On the fairing of resistance test data: A software-based approach     

Ali Can Takinaci   《Ocean Engineering》2006,33(17-18):2260-2269

A fairing method is presented for giving a good representation of ship model resistance test data, which in general tend to exhibit a scattered trend and need to be smoothed appropriately. One component of the test data originates from the frictional resistance and can be formulated smoothly. The second component is the wave resistance which exhibits a specific harmonic character with peaks and troughs. In this method, this component is modelled using suitable harmonic cosine terms for obtaining the best representation of the corresponding resistance data. The performance of the method is then tested on two example cases, using the characteristic parameters of the sample data as input during the smoothing process.  相似文献   

LAPP对中国对虾(Penaeus chinensis)生长、缺氧耐受力及免疫抵抗力的影响   总被引：11，自引：0，他引：11  

王伟庆  李爱杰 《海洋湖沼通报》1996,(1)

实验研究维生素C多聚磷酸酯（LAPP）添加在对虾饲料中，对中国对虾的生长、缺氧耐受力及免疫抵抗力的影响；实验进一步证明了中国对虾饲料中LAPP的最适添加量为400mg／100g饲料;本文还提出饲料中的LAPP能够提高中国对虾的缺氧耐受力，在溶氧超过3．1mg／kg海水的情况下，效果显著，在降低到2．3mg／kg海水时，作用减弱；用副溶血弧菌（Vibroparahaemolyticus）注射入虾体内，12小时内记录发现，饲料中不含LAPP的一组中国对虾，死亡率高于其它组，并且，LAPP含量最高的一组，死亡率明显低，死亡时间也出现较晚，这证明LAPP能够提高中国对虾的免疫抵抗力；本实验用注射金黄色葡萄球菌后细胞的吞噬百分率作指标，研究对虾细胞的吞噬作用，发现饲料中不含LAPP的对虾的吞噬百分率较低，随着饲料中LAPP浓度的提高，对虾血细胞的吞噬百分率逐渐升高，说明中国对虾细胞的吞噬作用逐步增强。  相似文献   

Voluntarism,defensive localism and spaces of resistance to health care restructuring     

《Geoforum》2016

This paper examines the evolving role of volunteers and the voluntary sector in shaping community responses to structural change in health care systems. It contributes to the emerging understanding of the different forms voluntarism can take within and among places, including as a space of resistance to contemporary restructuring initiatives such as regionalization. Within the geographies of voluntarism literature, however, little attention has been directed towards interrogating the local dynamics of such voluntarism, especially as it is reflected in public discourse. We address this deficiency through a media-based case study of public reaction to the recent implementation of Ontario’s Local Health Integration Networks (a type of regional health authority). Specifically, we examine a decade of newspaper coverage in a mid-size Canadian city region to document and characterize how the activities of volunteers and voluntary sector organizations in the community are portrayed in light of the structural imperatives to integrate health care services and regionalize health care governance. The media findings reveal a suite of public concerns and related activities, with the voluntary sector called upon in various ways to defend the autonomy of the community against the perceived threats to local services, employment and vulnerable populations. We interpret the evident complexity of voluntary sector resistance as a form of ‘defensive localism’ and discuss implications for developing informed policy on health care restructuring and for advancing knowledge on the local geographies of voluntarism.  相似文献   

研究气温变化趋势的新方法——三组耐抗线     

刘俊卿  杨军  强德厚 《干旱气象》2007,25(1):79-82

针对气象资料难以满足高斯分布的情况,引进了具有耐抗性的三组耐抗线方法,分析了满足不同信度情况下最小二乘回归和三组耐抗线的差异,并用西藏近30 a气温资料进行了检验。结果表明三组耐抗线方法比最小二乘法研究气候变化趋势有明显优势;采用最小二乘法得到的西藏气候变暖估计偏高。  相似文献   

Numerical calculation of free-surface potential flow around a ship using the modified Rankine source panel method     

Md. Shahjada Tarafder  Kazuo Suzuki 《Ocean Engineering》2008,35(5-6):536-544

A modified Rankine source panel method is presented for solving a linearized free-surface flow problem with respect to the double-body potential. The method of solution is based on the distribution of Rankine sources on the hull as well as its image and on the free surface. An iterative algorithm is used for determining the free surface and wave resistance using upstream finite difference operator. A verification of numerical modeling is made using the Wigley hull and the validity of the computer program is examined by comparing the details of wave profiles and wave making resistance with Series 60 model.  相似文献   

A critical CAE analysis of the bottom shape of a multi stepped air cavity planing hull     

《Applied Ocean Research》2019

One of the most important aspects, in the ACS and multi stepped hull design, is the choice of the geometrical shape of the cavity and the steps. In this article a complete experimental and numerical campaign on a multi stepped ACS has been carried out, varying the velocity and the air flow rate under the hull. The experimental tests have been conducted in an ITTC Towing Tank allowing to validate the numerical simulations obtained by means of a CFD U-RANSe (Unesteady Reynolds Averaged Navier-Stokes equations) code. The CFD setup is described in detail. From this campaign a critical analysis of the Froude number influence on the air cushion shape has been argued. The authors identified four different behaviours, from low to very high Froude numbers. The use of CFD has allowed to observe quantities of difficult evaluation by means of traditional experimental test, as e.g. the frictional component of the resistance, the airflow path lines and the volume of fraction in transversal and longitudinal sections. The results have been discussed.  相似文献   

Experimental study on hydrodynamic performance of SWATH vessels in calm water and in head waves     

《Applied Ocean Research》2019

An extensive experimental investigation on four SWATH hull forms has been conducted in calm water and in regular waves at University of Naples Federico II. Calm water tests have been analyzed in the range of Froude number Fr_T from 0.1 to 0.6. For all four SWATH configurations at the speed, corresponding to Fr_T 0.32, the behaviour in regular waves has been tested. The results of heave, pitch and vertical accelerations are presented in nondimensional form as RAO. For the “most promising” SWATH #4 configuration, a set of stabilizing fins have been designed and an active stabilization system has been developed. The developed SWATH#5 has been tested in calm water on three displacements in the range of Fr_T from 0.1 to 0.65. The dynamic wetted surface has been identified and the residual resistance coefficient C_R as well as R_T/Δ are reported. Seakeeping tests have been performed in regular head sea and in head and following irregular sea at Fr_T = 0.50. The conditions for the occurrence of dynamic longitudinal instabilities have been identified. The results allows to comment the effect of slenderness of struts and SWATH’s immersed bodies on resistance and seakeeping and concerns the applicability of SWATH concept to small craft.  相似文献   

Numerical study on scale effect of form factor     

Jen-Shiang Kouh  Shiu-Wu Chau 《Ocean Engineering》2009,36(5):403-413

The scale effect of form factor is investigated via a numerical approach in this paper, where the turbulent ship flow is computed by solving the steady and incompressible Reynolds-averaged Navier-Stokes and continuity equations. A wall function approach is employed to bridge the near-wall and outer turbulent flow region. The numerical scheme based on a finite-volume formulation is applied to discretize the coupled governing equation. For the sake of numerical stability, accuracy and economy, an identical grid is employed to compute ship flow at different Reynolds number, where the grid is optimized for the medium Reynolds number of the investigated range. Four surface ships and two sub-bodies with notably different geometrical characteristics are chosen as the investigated cases, where double-model flow without appendages is computed. The calculated total resistance coefficient shows a decreasing tendency against Reynolds number among all studied hulls. Similar to the calculated total resistance coefficient, the calculated friction resistance coefficient decreases with the Reynolds number and varies relatively little for a given Reynolds number among different hulls. The viscous pressure resistance coefficient is less insensitive to the Reynolds number but apparently depends on hull form. Compared with the form factor calculation based on empirical friction lines, the flat-plate friction prediction based on CFD approach clearly gives smaller Re-dependent form factor, which should more realistically reflect the scale effect of form factor. The form factor exhibits a near linear and increasing dependence on Reynolds number. The numerical results show that the dependence of r_P on Reynolds number mainly governs the scale effect of form factor.  相似文献   

Suction-controlled triaxial testing: Laboratory procedures in relation to resistance envelope methods     

M. G. Anderson  M. J. Kemp 《地球表面变化过程与地形》1987,12(6):649-654

In certain tropical slopes it appears possible that soil suction may play a significant role in maintaining stability. This communication outlines a laboratory suction-controlled triaxial test that can be undertaken to validate the threshold suction predicted by resistance envelope methods. It is shown that for two sites examined in St. Lucia, West Indies, the results from such a test confirm results obtained by use of resistance envelopes.  相似文献