The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)     

Xabier Arzuaga  Wanda Calcao  Adria Elskus 《Marine environmental research》2004,58(2-5):517

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.  相似文献   

On the fairing of resistance test data: A software-based approach     

Ali Can Takinaci   《Ocean Engineering》2006,33(17-18):2260-2269

A fairing method is presented for giving a good representation of ship model resistance test data, which in general tend to exhibit a scattered trend and need to be smoothed appropriately. One component of the test data originates from the frictional resistance and can be formulated smoothly. The second component is the wave resistance which exhibits a specific harmonic character with peaks and troughs. In this method, this component is modelled using suitable harmonic cosine terms for obtaining the best representation of the corresponding resistance data. The performance of the method is then tested on two example cases, using the characteristic parameters of the sample data as input during the smoothing process.  相似文献   

LAPP对中国对虾(Penaeus chinensis)生长、缺氧耐受力及免疫抵抗力的影响   总被引：11，自引：0，他引：11  

王伟庆  李爱杰 《海洋湖沼通报》1996,(1)

实验研究维生素C多聚磷酸酯（LAPP）添加在对虾饲料中，对中国对虾的生长、缺氧耐受力及免疫抵抗力的影响；实验进一步证明了中国对虾饲料中LAPP的最适添加量为400mg／100g饲料;本文还提出饲料中的LAPP能够提高中国对虾的缺氧耐受力，在溶氧超过3．1mg／kg海水的情况下，效果显著，在降低到2．3mg／kg海水时，作用减弱；用副溶血弧菌（Vibroparahaemolyticus）注射入虾体内，12小时内记录发现，饲料中不含LAPP的一组中国对虾，死亡率高于其它组，并且，LAPP含量最高的一组，死亡率明显低，死亡时间也出现较晚，这证明LAPP能够提高中国对虾的免疫抵抗力；本实验用注射金黄色葡萄球菌后细胞的吞噬百分率作指标，研究对虾细胞的吞噬作用，发现饲料中不含LAPP的对虾的吞噬百分率较低，随着饲料中LAPP浓度的提高，对虾血细胞的吞噬百分率逐渐升高，说明中国对虾细胞的吞噬作用逐步增强。  相似文献   

Experimental study on hydrodynamic performance of SWATH vessels in calm water and in head waves     

《Applied Ocean Research》2019

An extensive experimental investigation on four SWATH hull forms has been conducted in calm water and in regular waves at University of Naples Federico II. Calm water tests have been analyzed in the range of Froude number Fr_T from 0.1 to 0.6. For all four SWATH configurations at the speed, corresponding to Fr_T 0.32, the behaviour in regular waves has been tested. The results of heave, pitch and vertical accelerations are presented in nondimensional form as RAO. For the “most promising” SWATH #4 configuration, a set of stabilizing fins have been designed and an active stabilization system has been developed. The developed SWATH#5 has been tested in calm water on three displacements in the range of Fr_T from 0.1 to 0.65. The dynamic wetted surface has been identified and the residual resistance coefficient C_R as well as R_T/Δ are reported. Seakeeping tests have been performed in regular head sea and in head and following irregular sea at Fr_T = 0.50. The conditions for the occurrence of dynamic longitudinal instabilities have been identified. The results allows to comment the effect of slenderness of struts and SWATH’s immersed bodies on resistance and seakeeping and concerns the applicability of SWATH concept to small craft.  相似文献   

Voluntarism,defensive localism and spaces of resistance to health care restructuring     

《Geoforum》2016

This paper examines the evolving role of volunteers and the voluntary sector in shaping community responses to structural change in health care systems. It contributes to the emerging understanding of the different forms voluntarism can take within and among places, including as a space of resistance to contemporary restructuring initiatives such as regionalization. Within the geographies of voluntarism literature, however, little attention has been directed towards interrogating the local dynamics of such voluntarism, especially as it is reflected in public discourse. We address this deficiency through a media-based case study of public reaction to the recent implementation of Ontario’s Local Health Integration Networks (a type of regional health authority). Specifically, we examine a decade of newspaper coverage in a mid-size Canadian city region to document and characterize how the activities of volunteers and voluntary sector organizations in the community are portrayed in light of the structural imperatives to integrate health care services and regionalize health care governance. The media findings reveal a suite of public concerns and related activities, with the voluntary sector called upon in various ways to defend the autonomy of the community against the perceived threats to local services, employment and vulnerable populations. We interpret the evident complexity of voluntary sector resistance as a form of ‘defensive localism’ and discuss implications for developing informed policy on health care restructuring and for advancing knowledge on the local geographies of voluntarism.  相似文献   

Suction-controlled triaxial testing: Laboratory procedures in relation to resistance envelope methods     

M. G. Anderson  M. J. Kemp 《地球表面变化过程与地形》1987,12(6):649-654

In certain tropical slopes it appears possible that soil suction may play a significant role in maintaining stability. This communication outlines a laboratory suction-controlled triaxial test that can be undertaken to validate the threshold suction predicted by resistance envelope methods. It is shown that for two sites examined in St. Lucia, West Indies, the results from such a test confirm results obtained by use of resistance envelopes.  相似文献   

FORECASTING IMPACTS OF HYDRAULIC STRUCTURESON AN ALLUVIAL RIVER     

LIN Bingnan 《国际泥沙研究》1992,(1)

In the last decades the construction of large hydraulic projects on alluvial rivers in China has spurred the development of both physical and one-dimensional mathematical modeling to the point that deposition and erosion predicted by modeling in either way conform to prototype observations over periods of 3 to 19 years. The prototype data are those of Gezhouba on the Yangtze River and Danjiangkou on the Han River. The accuracy attained is in the neighborhood of 20% which is considered good enough in sedimentation engineering. Thus these techniques of modeling may be applied to forecast the impacts of hydraulic structures on an alluvial river. Mathematical modeling in 2 space variables has also been developed, but still awaits further verification.  相似文献   

Occurrence and Resistance of Pathogenic Bacteria Along the Tietê River Downstream of São Paulo in Brazil     

Wolf-Rainer Abraham  Alexandre José Macedo  Luiz Humberto Gomes  Flavio C. A. Tavares 《洁净——土壤、空气、水》2007,35(4):339-347

The load of pathogenic bacteria, their fate and their dangerousness in the Tietê River were assessed along 100 km starting from the city of São Paulo, Brazil. The data were compared with those from two German rivers. High loads of pathogens were found in the Tietê River near the city of São Paulo (Escherichia coli O157:H7, Shigella flexneri, and Shigella boydii), which were absent 30 km downstream of São Paulo. The antibiotic resistances observed in the Tietê river were rather low and decreased after the major input in São Paulo to significantly lower levels about 30 km downstream. While the Brazilian isolates were more susceptible for ampicillin than the German ones, the reverse was observed for gentamycin. For optimal control of infections in humans critical areas where these bacteria survived longer and their elimination mechanisms should be identified as well as the extent and the origin of antibiotic resistance should be determined.  相似文献   

研究气温变化趋势的新方法——三组耐抗线     

刘俊卿  杨军  强德厚 《干旱气象》2007,25(1):79-82

针对气象资料难以满足高斯分布的情况,引进了具有耐抗性的三组耐抗线方法,分析了满足不同信度情况下最小二乘回归和三组耐抗线的差异,并用西藏近30 a气温资料进行了检验。结果表明三组耐抗线方法比最小二乘法研究气候变化趋势有明显优势;采用最小二乘法得到的西藏气候变暖估计偏高。  相似文献   

Velocity profiles and bed shear stress over various bed configurations in a river bend     

John S. Bridge  Jack Jarvis 《地球表面变化过程与地形》1977,2(4):281-294

Vertical velocity profiles measured over various bed configurations (plane beds, ripples, and dunes) in. the meandering River South Esk, Glen Clova, Scotland are presented on semilogarithmic paper. Local bed shear stress and roughness height are calculated from the lowermost parts of the profiles using the Karman-Prandtl law of the wall; these parameters, and the geometrical properties of the profiles, are related to the various bed configurations. A graphical model is used to identify profiles developed on specific regions of dune geometry, in order to discriminate those profiles that define bed shear effective in transporting sediment over dunes. An assessment is made of the errors involved in estimating local mean velocity from extrapolating the law of the wall to the water surface. A Darcy-Weisbach friction coefficient is related to bed configuration and local stream power.  相似文献