CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

黄河口及渤海中南部沉积物工程特性及其机理   总被引：3，自引：0，他引：3  

郑继民  沈谓铨  陆念祖  冯秀丽 《中国海洋大学学报(自然科学版)》1994,(2)

采用Ｋａｓｔｅｎ取样器从黄河口及渤海湾中南部海底沉积物中取样，对样品通过土工性质、微结构研究以及粘土矿物分析，阐明本研究区三维空间展布的海底沉积物形成机理及其工程地质规律，论证水动力作用和斜坡不稳定性对工程特性和微结构的重要影响．  相似文献   

红笛鲷头肾和脾脏显微结构的观察   总被引：7，自引：0，他引：7  

王鸿鹄  李长玲  曹伏君  简纪常 《台湾海峡》2006,25(3):360-367

通过对红笛鲷头肾和脾脏显微结构的观察发现，头肾表面覆盖有一薄层纤维结缔组织性被膜，未见明显的小梁．实质主要由淋巴组织和血窦构成，可分为两个区，A区淋巴组织排列成索状，环血管呈放射状分布；B区的淋巴细胞则以形成弥散性分布淋巴组织为特征．主要细胞有各种血细胞、巨噬细胞和一些其他的颗粒细胞等，未见明显的巨噬细胞聚集中心及粒细胞聚集中心．脾脏被膜较薄，未见明显的小梁，实质由白髓和红髓相间排列组成．白髓中未见明显的脾小结、淋巴鞘结构，红髓由脾索和脾窦构成．研究结果表明：红笛鲷的头肾和脾脏是硬骨鱼类机体造血的主要器官，又是鱼类重要的免疫器官．  相似文献   

海冰板的环形屈曲的压缩破坏   总被引：2，自引：0，他引：2  

唐立强  陈会军等 《海洋工程》2001,19(4):29-33

在轴对称条件下，给出海冰板的环向屈曲面临载荷，从细观力学的观点分析了微裂纹开裂的条件，扩展方向并给出绕海洋平台柱腿冰的损伤区的尺度。所给出的临界半径是一个与冰材料力学特征，冰的微结构参数一海洋平台柱腿半径相关的特征参数。对现场所观察和测量的结构给出了解释和定量分析。  相似文献   

土体微观结构定量分析系统及应用   总被引：8，自引：0，他引：8  

张礼中  胡瑞林  李向全  张永波 《地质科技情报》2008,27(1):108-112

土体微观结构图像的计算机处理是土体微观结构定量化研究的关键技术之一.在分析我国土体微观结构定量研究的进展的基础上,针对土体微观结构图像计算机处理软件对土体微观结构非确定型参数定量分析功能的不足,设计和开发了新版土体微观结构定量分析系统,以太原高阶地黄土为例,通过各夯击次数对应土样的定量化结构效应分析,探讨了其动力固结的微观结构变化规律.该系统的应用对推动我国土体微观结构的研究具有积极意义.  相似文献   

岩石电子背散射衍射（EBSD）组构分析及应用   总被引：18，自引：1，他引：17  

刘俊来  曹淑云  邹运鑫  宋志杰 《地质通报》2008,27(10):1638-1645

电子背散射衍射（EBSD）技术是现代构造地质学与显微构造分析领域一项崭新的技术,它与现代高分辨率扫描电子显微镜和能谱分析设备结合,可以对块状样品中微米或纳米级尺度的颗粒进行晶体结构分析, 从而使微观结构、微区成分与结晶学数据结合起来,能够更精细地对比研究矿物和岩石显微构造,为岩石显微构造分析开辟了一个全新的领域。分析了EBSD技术的基本原理、系统构成、样品制备和工作程序。介绍了石英组构测量,极细粒物质（微角砾岩）的组构特点,二轴晶矿物（角闪石）的组构、变形机制和金属硫化物组构分析的应用实例,对于应用EBSD系统开展研究存在的一些问题进行了讨论。  相似文献   

Micro-fracture instabilities in granular solids     

Gabriela Bilbie  Cristian Dascalu  Rene Chambon  Denis Caillerie 《Acta Geotechnica》2008,3(1):25-35

We study the aspect of unstable behavior (like strain localization bands) in elastic solids as a consequence of micro-fracturing. A two-scale approach of computational homogenization is considered. The macroscopic behavior is investigated by finite element computations on a unit cell. At the micro-level, we consider a granular structure with elastic grains. The inter-granular boundaries are modeled with cohesive laws, friction and unilateral contact. We show that decohesion between grains gives rise to macro-instabilities, indicated by the loss of ellipticity, typical for deformation localization bands. The relation between the microscopic softening on inter-granular boundaries and the onset of macro-instabilities is studied through numerical examples. The influence of the cohesive law and friction parameters is analyzed. For periodic distributions of granular structures, we prove the loss of periodicity by failure and the corresponding size dependence effect in the homogenized response. We present numerical examples of bifurcation of solutions for granular cell structures and of particular solutions specific to elementary volumes with periodic cell distribution. Size dependence appears in the unstable regime and is strongly influenced by cohesion and friction parameters.  相似文献   

Dual simulations of fluid flow and seismic wave propagation in a fractured network: effects of pore pressure on seismic signature   总被引：1，自引：0，他引：1  

S. Vlastos  E. Liu  I. G. Main  M. Schoenberg  C. Narteau  X. Y. Li  B. Maillot 《Geophysical Journal International》2006,166(2):825-838

  相似文献   

A cellular model of Holocene upland river basin and alluvial fan evolution   总被引：1，自引：0，他引：1  

T. J. Coulthard  M. G. Macklin  M. J. Kirkby 《地球表面变化过程与地形》2002,27(3):269-288

The CAESAR (Cellular Automaton Evolutionary Slope And River) model is used to simulate the Holocene development of a small upland catchment (4·2 km²) and the alluvial fan at its base. The model operates at a 3 m grid scale and simulates every flood over the last 9200 years, using a rainfall record reconstructed from peat bog wetness indices and land cover history derived from palynological sources. Model results show that the simulated catchment sediment discharge above the alluvial fan closely follows the climate signal, but with an increase in the amplitude of response after deforestation. The important effects of sediment storage and remobilization are shown, and findings suggest that soil creep rates may be an important control on long term (>1000 years) temperate catchment sediment yield. The simulated alluvial fan shows a complex and episodic behaviour, with frequent avulsions across the fan surface. However, there appears to be no clear link between fan response and climate or land use changes suggesting that Holocene alluvial fan dynamics may be the result of phases of sediment storage and remobilization, or instabilities and thresholds within the fan itself. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

基于神经网络的单元自动机CA及真实和忧化的城市模拟   总被引：6，自引：0，他引：6  

黎夏  叶嘉安 《地理学报》2002,(2)

提出了一种基于神经网络的单元自动机（ＣＡ）。ＣＡ已被越来越多地应用在城市及其它地理现象的模拟中。ＣＡ模拟所碰到的最大问题是如何确定模型的结构和参数。模拟真实的城市涉及到使用许多空间变量和参数。当模型较复杂时，很难确定模型的参数值。本模型的结构较简单，模型的参数能通过对神经网络的训练来自动获取。分析表明，所提出的方法能获得更高的模拟精度，并能大大缩短寻找参数所需要的时间。通过筛选训练数据，本模型还可以进行优化的城市模拟，为城市规划提供参考依据。  相似文献