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1.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引:4,自引:0,他引:4  
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   
2.
饶长辉  姜文汉  凌宁 《天文学报》2001,42(2):134-139
分析以观测系统焦面上和探测器测到的太阳表面米粒结构的对比度与观测系统口径,大气湍流相干长度以及系统探测灵敏度的关系,给出了不同口径,不同大气湍流相干长度以及不同系统探测灵敏度时的米粒结构对比值计算结果,此外还给出了实验结果。  相似文献   
3.
海洋平台沉垫的波浪载荷试验研究   总被引:2,自引:0,他引:2  
本文通过对海洋平台沉垫的波浪载荷试验研究分析,着重研究了在规则波中平台沉垫总体波浪载荷和局部水动压力分布的情况,定性地考察了非线性影响、浅水影响的重要性,为今后波浪理论中粘性的引入,进而考虑粘性与波浪运动的非线性相互干扰提供实测依据  相似文献   
4.
The spatial and temporal dynamics of dissolved inorganic nitrogen, dissolved phosphate, dissolved silica and chlorophyll a were measured seasonally at eight stations in the Ria de Aveiro. Between December 2000 and September 2001, the seasonal succession of phytoplankton assemblages, inferred after the spatial and seasonal variation of silica and of chlorophyll a concentrations, showed that diatoms (μmol Si L−1) dominated from late autumn until early spring, while chlorophytes (μg Chl a L−1) bloomed during late spring and summer. The Si:N:P ratios and Si concentrations indicated no seasonal depletion in dissolved silica, as in other temperate systems, possibly because of abnormal precipitation and flood events prolonging the supply of dissolved Si to the system. The Si:N:P ratios suggested P limitation at the system level. Despite the relative proportions of available nutrients, the mean phosphorus concentration (5.3 μmol L−1) was above the reported half-saturation constants for P uptake by phytoplankton. Thus, in Ria de Aveiro, the seasonal succession of phytoplankton assemblages may also be dependent on the grazing capacity of the pelagic community through top-down regulation.  相似文献   
5.
We introduce and propose zircon M257 as a future reference material for the determination of zircon U‐Pb ages by means of secondary ion mass spectrometry. This light brownish, flawless, cut gemstone specimen from Sri Lanka weighed 5.14 g (25.7 carats). Zircon M257 has TIMS‐determined, mean isotopic ratios (2s uncertainties) of 0.09100 ± 0.00003 for 206pb/238U and 0.7392 ± 0.0003 for 207pb/235U. Its 206pb/238U age is 561.3 ± 0.3 Ma (unweighted mean, uncertainty quoted at the 95% confidence level); the U‐Pb system is concordant within uncertainty of decay constants. Zircon M257 contains ~ 840 μg g?1 U (Th/U ~ 0.27). The material exhibits remarkably low heterogeneity, with a virtual absence of any internal textures even in cathodoluminescence images. The uniform, moderate degree of radiation damage (estimated from the expansion of unit‐cell parameters, broadening of Raman spectral parameters and density) corresponds well, within the “Sri Lankan trends”, with actinide concentrations, U‐Pb age, and the calculated alpha fluence of 1.66 × 1018 g?1. This, and a (U+Th)/He age of 419 ± 9 Ma (2s), enables us to exclude any unusual thermal history or heat treatment, which could potentially have affected the retention of radiogenic Pb. The oxygen isotope ratio of this zircon is 13.9%o VSMOW suggesting a metamorphic genesis in a marble or calc‐silicate skarn.  相似文献   
6.
Among numerous methods for cation exchange capacity (CEC) determination for soils and sediments, the cobaltihexamine chloride method is frequently used due to its ability to measure CEC at soil pH. After exchange with Co(NH3)63+ ions, CEC is estimated via the measurement of the Co remaining in solution. The modified method proposed allows a more rapid determination of CEC based on the measurement of the absorbance at 472 nm of the cobaltihexamine chloride solution before and after exchange. This method has been applied to various soil's horizons from four sites, selected to cover a wide range of CEC and pH values. The model obtained allows one to calculate CEC from absorbance at 472 nm with 95% confidence intervals. As CEC is of relevant meaning in agronomical and environmental purposes, and more recently in ecotoxicological studies, this modified method can be proposed as a rapid test for CEC evaluation.  相似文献   
7.
Ice and snow have often helped physicists understand the world. On the contrary it has taken them a very long time to understand the flow of the glaciers. Naturalists only began to take an interest in glaciers at the beginning of the 19th century during the last phase of glacier advances. When the glacier flow from the upslope direction became obvious, it was then necessary to understand how it flowed. It was only in 1840, the year of the Antarctica ice sheet discovery by Dumont d'Urville, that two books laid the basis for the future field of glaciology: one by Agassiz on the ice age and glaciers, the other one by canon Rendu on glacier theory. During the 19th century, ice flow theories, adopted by most of the leading scientists, were based on melting/refreezing processes. Even though the word ‘fluid’ was first used in 1773 to describe ice, more the 130 years would have to go by before the laws of fluid mechanics were applied to ice. Even now, the parameter of Glen's law, which is used by glaciologists to model ice deformation, can take a very wide range of values, so that no unique ice flow law has yet been defined. To cite this article: F. Rémy, L. Testut, C. R. Geoscience 338 (2006).  相似文献   
8.
Analysis of monthly mean river temperatures, recorded on an hourly basis in the middle reaches of the Loire since 1976, allows reconstruction by multiple linear regression of the annual, spring and summer water temperatures from equivalent information on air temperatures and river discharge. Since 1881, the average annual and summer temperatures of the Loire have risen by approximately 0.8?°C, this increase accelerating since the late 1980s due to the rise in air temperature and also to lower discharge rates. In addition, the thermal regime in the Orleans to Blois reach is considerably affected by the inflow of groundwater from the Calcaires de Beauce aquifer, as shown by the summer energy balance. To cite this article: F. Moatar, J. Gailhard, C. R. Geoscience 338 (2006).  相似文献   
9.
The Oued Bahloul structure exists in the central Atlas of Tunisia. This structure is oriented N70 and it shows a very particular geometry characterized by: (i) an important disharmony between the northern and the southern flanks; (ii) a dissymmetry between the east and the west parts of the structure; (iii) several gaps and progressive unconformities present in the Cretaceous and Eocene series.Three major tectonic directions characterize this structure: (i) the N120 appears in the western part of the anticline; (ii) some north–south faults distinguish the oriental part; (iii) the strike fault, which is confirmed by seismic data, affects the central part of this anticline. To cite this article: A. Saadi et al., C. R. Geoscience 338 (2006).  相似文献   
10.
The purpose of this study is to develop a geotechnical microzonation model using Geographical Information Systems (GIS) based on Multicriteria Decision Analysis (MCDA). As study area, the Eskişehir downtown area has been chosen. Eskişehir is one of the most rapidly growing cities in central Turkey. The model inputs include slope, flood susceptibility, soil, depth to groundwater table, swelling potential, and liquefaction potential. The weight and rank values are assigned to the layers and to the classes of each layer respectively. The assignment of the weight/rank values and the analysis are realized by the application of two different decision models, namely Simple Additive Weighting (SAW) and Analytic Hierarchy Process (AHP) methods. The geotechnical microzonation maps prepared as outputs of these methods are found to be consistent with each other and confirmed by the experts within the study area. The geotechnical microzonation map prepared using the AHP method is recommended as the final map of the study.  相似文献   
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