Composition and distribution of phytoplankton with size fraction results at southwestern east/japan sea     

Mi Ok Park 《Ocean Science Journal》2006,41(4):301-313

Abundance and distribution of phytoplankton in seawater at southwestern East/Japan Sea near Gampo were investigated by HPLC analysis of photosynthetic pigments during summer of 1999. Detected photosynthetic pigments were chlorophyll a, b, c₁₊₂ (Chl a, Chl b, Chl c₁₊₂), fucoxanthin (Fuco), prasinoxanthin (Pras), zeaxanthin (Zea), 19’-butanoyloxyfucoxanthin (But-fuco) and beta-carotene (β-Car). Major carotenoid was fucoxanthin (bacillariophyte) and minor carotenoids were Pras (prasinophyte), Zea (cyanophyte) and But-fuco (chrysophyte). Chl a concentrations were in the range of 0.16-8.3/land subsurface chlorophyll maxima were observed at 0-10m at inshore and 30–50 m at offshore. Thermocline and nutricline tilted to the offshore direction showed a mild upwelling condition. Results from size-fraction showed that contribution from nano+picoplankton at Chl a maximum layer was increased from 18% at inshore to 69% at offshore on average. The maximum contribution from nano+picoplankton was found as 87% at St. E4. It was noteworthy that contribution from nano+picoplanktonic crysophytes and green algae to total biomass of phytoplankton was significant at offshore. Satellite images of sea surface temperature indicated that an extensive area of the East/Japan Sea showed lower temperature (<18 °C) but the enhanced Chl a patch was confined to a narrow coastal region in summer, 1999. Exceptionally high flux of low saline water from the Korea/Tsushima Strait seemed to make upwelling weak in summer of 1999 in the study area. Results of comparisons among Chl a from SeaWIFs, HPLC and fluorometric analysis showed that presence of Chl b cause underestimation of Chl a about 30% by fluorometric analysis but overestimation by satellite data about 30-75% compared to HPLC data.  相似文献   

2000年夏季莱州湾生态环境要素的分布特征   总被引：1，自引：0，他引：1  

高会旺  吴德星  白洁  石金辉  李正炎  江文胜 《中国海洋大学学报(自然科学版)》2003,33(2):185-191

论文依据 2 0 0 0年夏季 8月 2 9日至 9月 2日莱州湾的 1次综合性生态环境调查资料 ,给出了表、中、底 3层的平均温度、盐度、主要营养盐浓度及其比例和叶绿素 a浓度 ,分析了莱州湾的温盐结构、主要营养盐和叶绿素 a的分布特征。由于莱州湾的水深较浅 ,各要素的垂直分布都比较均匀。生态环境要素的水平分布表现为小清河口为高温、低盐、高营养盐和高叶绿素 a浓度区 ,小清河口东测的湾顶区域为高温、高盐、相对低的营养盐和叶绿素 a浓度区。此次观测到的盐度较 1997年以前有明显升高。莱州湾各层平均的 N/ P和 Si/ N分别为 16.73和 1.67,都比 1998～ 1999年渤海中部的值大 ,但 N/ P比 1992 ,1995和 1996年莱州湾的 N/ P明显偏低。叶绿素 a浓度与硅酸盐浓度之间有较好的相关关系 (α=0 .0 1) ,表、中、底 3层叶绿素 a浓度同硅酸盐浓度的线性相关系数分别为 :0 .5 4 ,0 .68和 0 .67  相似文献   

长江口及其邻近海域叶绿素a分布特征及其与低氧区形成的关系   总被引：2，自引：0，他引：2  

李照  宋书群  李才文 《海洋科学》2016,40(2):1-10

于2013年3月和8月研究了长江口及其邻近海域叶绿素a的分布特征,并对环境因子和长江冲淡水对浮游植物生物量分布的影响进行了探讨。结果表明,叶绿素a浓度在丰水期较高,平均值为5.18μg/L,最高值达32.05μg/L,现场海水出现变色现象;与同期历史资料对比分析,发现该海域叶绿素a浓度呈现出波动增长趋势。丰水期与枯水期叶绿素a的相对高值区均位于冲淡水的中部,122.5°E~123°E之间;丰水期在调查海域出现溶解氧低值区与低氧区,最低值仅为0.64 mg/L;发现低氧区出现位置北移、面积扩大和溶解氧最低值下降的趋势。底层溶解氧低值区分布与表层叶绿素高值区大致吻合,表明低氧现象与表层浮游植物的生长和现存量密切相关,在跃层存在的水体中表层浮游植物的大量繁殖易造成底层低氧区的出现。  相似文献   

Inhibitory activities of microalgal fucoxanthin againstα-amylase,α-glucosidase,and glucose oxidase in 3T3-L1cells linked to type 2 diabetes     

KAWEE-AI Arthitaya  KIM Aaron Taehwan  KIM Sang Moo 《海洋湖沼学报（英文）》2019,(3):928-937

Postprandial hyperglycemia is an early indication of type 2 diabetes and the target of many anti-diabetic and anti-obesity studies.α-Glucosidase and α-amylase are the crucial factors in regulating starch digestion and glucose absorption,making them key targets for many studies to treat postprandial hyperglycemia.We studied the inhibitory activities of microalgal fucoxanthin against rat-intestinalα-glucosidase and pancreaticα-amylase along with the antidiabetic eff ect to induce diff erentiation in 3T3-L1 pre-adipocytes using Oil Red-O staining.Fucoxanthin displayed strong hindrance activities towardα-amylase in a concentration-dependent manner,with an IC50 value of 0.68mmol/L,whereas weak inhibitory activity against α-glucosidase,with an IC 50 value of 4.75 mmol/L.Fucoxanthin also considerably elevated glucose oxidase activity in 3T3-L1 cells by 31.3%at 5μmol/L.During adipocyte differentiation,fucoxanthin showed lipid accumulation in 3T3-L1 cells with no cytotoxicity up to 20μmol/L.However,fucoxanthin had no inhibitory activity on glucose-6-phosphate dehydrogenase.These results suggest that fucoxanthin might be useful for the prevention of obesity or diabetes by inhibiting carbohydrate-hydrolyzing enzymes and lipid accumulation and be utilized as an ingredient for a functional food or dietary supplement.  相似文献   

壳聚糖/α,β-甘油磷酸凝胶微球的制备及性质研究     

臧惠迪  程晓杰  李阳  李敬  陈西广 《中国海洋大学学报(自然科学版)》2012,(9):35-40

以液体石蜡为油相,以壳聚糖/α,β-甘油磷酸凝胶溶液为水相,用乳化法制备了壳聚糖/α,β-甘油磷酸凝胶微球,并对微球形态、粒径分布、溶胀性、蛋白吸附率、溶血率、体外降解率进行了分析。光学显微镜下显示,微球形态圆整,粒径主要分布在100～400μm;扫描电镜显示,微球内部为三维网络状结构,骨架清晰;pH=7.4的PBS缓冲液中干凝胶微球的溶胀率为218.08%,普通壳聚糖微球为70.98%;1和24h测得凝胶微球的蛋白吸附量分别为13.21和15.68μg/g,普通壳聚糖微球分别为3.71×103和4.83×103μg/g;微球的溶血率小于5%,血液相容性良好;体外降解实验显示,壳聚糖的脱乙酰度、黏度以及溶菌酶浓度对壳聚糖/α,β-甘油磷酸凝胶微球的降解性质产生影响。  相似文献   

Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians          下载免费PDF全文

Miguel Costa Leal  Bruno Jesus  João Ezequiel  Ricardo Calado  Rui Jorge Miranda Rocha  Paulo Cartaxana  João Serôdio 《Marine Ecology》2015,36(3):572-584

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.  相似文献   

浮游植物化学分类法—CHEMTAX运算在南中国海应用的改进和优化   总被引：3，自引：1，他引：2  

王磊  黄邦钦  柳欣  肖武鹏 《海洋学报(英文版)》2015,34(2):124-131

In order to determine the phytoplankton community composition, the modification and optimizing of the CHEMical TAXonomy (CHEMTAX) running was carried out through samples grouping, successive run and ev...  相似文献   

Temporal and spatial distribution characteristics of nutrients in Clarion-Clipperton Fracture Zone in the Pacific in 2017     

Baohong Chen  Kaiwen Zhou  Kang Wang  Jigang Wang  Sumin Wang  Xiuwu Sun  Jinmin Chen  Cai Lin  Hui Lin 《海洋学报(英文版)》2022,41(1):1-2

This research investigated eight stations in Clarion-Clipperton Fracture Zone(CCFZ) in the eastern tropical Pacific in 2017 to study the spatial distribution characteristics of nutrients and chlorophyll a(Chl a) concentration,and compared nutrient concentrations and molar ratios with those of other investigations 20 years ago in the same area. The study found that dissolved inorganic nutrient(N, P and Si) concentrations were lowest in the upper layer, and increased from surface to some depths, t...  相似文献   

Flows within the Ekman layer during spin-up of a thermally stratified fluid     

Jae Min Hyun 《地球物理与天体物理流体动力学》2013,107(1-4):65-79

Abstract

Finite-difference numerical solutions were obtained to present the flow and temperature field details within the transient Ekman layer during spin-up of a thermally stratified fluid in a cylinder. This complements the earlier studies on stratified spin-up which examined the flows in the interior core region. As the stratification increases, the following changes in the flow field are noticeable. The radial velocity in the Ekman layer decreases in magnitude. The azimuthal flows adjust smoothly from the interior region to the endwall boundary, and the Ekman layer in the azimuthal flow field fades. Vertical motions are inhibited, resulting in a weakened Ekman pumping. The axial vorticity field behaves similarly to the azimuthal flows. The temperature deviation from the equilibrium profile decreases, and the heat transfer flux from the endwall to the fluid decreases. The thickness of the thermal layer is larger than the velocity layer thickness. Illustrative comparisons of the relative sizes of the terms in the governing equations are conducted in order to assess the stratification effect in the adjustment process of the fluid.  相似文献   

Distribution characteristics of size-fractionated chlorophyll a and primary productivity in Beibu Gulf   总被引：2，自引：1，他引：1  

Liu Zilin  Cai Yuming  Ning Xiuren 《海洋学报(英文版)》1998,17(1):71-83

The distribution of the abundance of phytoplankton, chlorophyll a (Chl.a) concentration and primary productivity in the Beibu Gulf were observed from May 23 to June 4, 1994.The results show that there were marked featares of spatial zonation in the survey area due to the differences between the geographic environment and the hydrological conditions.Chlorophyll a and primary productivity were higher in the inshore than in the middle area and higher in the north than in the south of the Beibu Gulf.The average concentration of Chl.a, primary productivity and the abundance of phytoplankton were 0.94±0.45 μg/dm³, 351±172 mg/(m²·d) (C) and 0.97×10⁴-10.050×10⁴ ind./m³ in the area, respectively.There were 176 species belonging to 4 phyla and 56 genera based on microscope identification.The results of the size-fractionation show that the contribution of nanoplankton and picoplankton was 77% to total Chl.a and 91% to total primary productivity, which proved their importance to phytoplankton communities in the Beibu Gulf.  相似文献