Efficient gusA Transient Expression in Porphyra yezoensis Protoplasts Mediated by Endogenous Beta-tubulin Flanking Sequences     

GONG Qianhong)  YU Wengong)  *  DAI Jixun)  LIU Hongquan)  )  XU Rifu)  GUAN Huashi)  and PAN Kehou) ) College of Medicine  Pharmaceutics  Ocean University of China  Qingdao  P. R. China ) College of Marine Life Sciences  Ocean University of China  Qingdao  P. R. China ) College of Fisheries  Ocean University of China  Qingdao  P. R. China 《中国海洋大学学报(英文版)》2007,6(1):21-25

1 Introduction Porphyra yezoensis is one of the most widely con-sumed and cultivated seaweeds in the world. Over the past decades, much effort has been made in improving the cultured stocks and developing breeding techniques of alga. At present, nuclear transformation systems of eu-karyotic algae such as green unicellular alga Chlamydo-monas reinhardii (Rochaix and Dillewijn, 1982), diatom Phseodactylum tricornutum (Lioudmila et al., 2000) and simple multicellular green alga Volvox carteri (…  相似文献   

Efficient gusA transient expression in Porphyra yezoensis protoplasts mediated by endogenous beta-tubulin flanking sequences     

Gong Qianhong  Yu Wengong  Dai Jixun  Liu Hongquan  Xu Rifu  Guan Huashi  Pan Kehou 《中国海洋大学学报(英文版)》2007,6(1):21-25

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角叉菜(Chondrus ocellatus Holm)原生质体分离、培养与再生的研究     

张全启 《中国海洋大学学报(自然科学版)》1991,(1)

从角叉菜(Chondrus ocellatus Holm)的孢子体和雌配子体中,分离了大量的成活原生质体。分离的最适条件为:纤维素酶3％,粉红鲍鱼酶8％,山梨醇1.2mol/dm~3,pH6.0,温度为15℃。细胞壁的完全解离,已用荧光增白剂的染色不着色和原生质体在低渗溶液中的易碎性证实。培养后再生的细胞,经均等分裂,随后发育成实心球状体;或出芽后不等分裂,发育成丝状体和愈伤组织状细胞团。在PES培养基中,经30～40d培养,部分球状体和愈伤组织状细胞团分化出完整植株,但丝状体未能分化。低浓度的IAA、6—FA和C—751对球状体和愈伤组织状细胞团的生长和分化都有促进作用。由原生质体培养获得完整的再生植株,这在真红藻纲(Florideophyceae)中为首例报道。  相似文献