Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress   总被引：4，自引：0，他引：4  

郭振宇焦传珍  相建海 《中国海洋湖沼学报》2004,22(4):386-391

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.  相似文献   

Influence of Ultrasonic Treatment on the Allergenic Properties of Shrimp （Penaeus vannamei ） Allergen     

LI Zhenxing LIN Hong CAO Limin 《中国海洋大学学报(英文版)》2006,5(2):115-118

The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0 ℃ or 50 ℃ for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA （Ci-ELISA） to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0℃. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50 ℃. In the determination of allergenicity with CiELISA, a reduction in IgE binding was also observed.  相似文献   

Effects of Different Diets on the Dietary Attractability and Selectivity of Chinese Shrimp, Fenneropenaeus chinensis     

HUANGGuoqiang DONGShuanglin WANGFang 《中国海洋大学学报(英文版)》2005,4(1):56-60

Attractabilities of different diets and dietary selectivity of Chinese shrimp, Fenneropenaeus chinensis were studied through behavior observation and feeding experiment, respectively. The five diets used in the experiment are: Fish Flesh (FF), Shrimp Flesh (SF), Clam Foot (CF), Polychaete Worm (PW), and Formulated Diet (FD). No significant differences of attractability exist between any two different diets when every two natural diets or all five diets are provided simultaneously. On the other hand, significant differences of attractability exist between FD and every single natural diet when they are provided simultaneously. Results of behavioral observation indicate that natural diets are more attractive than FD. In feeding experiment, Chinese shrimp has distinct selectivity on different diets. It positively selects CF and PW, negatively selects FF and SF, and excludes FD absolutely. The results of the present studies indicate that the dietary selectivity of shrimp was based not only on the attractabilities of the diets, but also on the responses such as growth and food conversion.  相似文献   

对虾养殖生态系中有机碳的初步研究本研究由   总被引：1，自引：0，他引：1  

游奎  马甡 《中国海洋大学学报(自然科学版)》2002,32(1):51-55

对于围隔养殖对虾生态系中有机碳的变动进行了研究 ,结果表明 :溶解有机碳 (DOC)含量波动在 5 .2 99～ 13.39mg/ L之间 ,平均为 8.5 3mg/ L± 2 .2 5 mg/ L;颗粒有机碳 (POC)含量波动在0 .6 5～ 6 .6 3mg/ L之间 ,平均为 3.2 5 mg/ L± 1.76 mg/ L;总有机碳 (TOC)含量波动在 6 .92～ 2 0 .0 2mg/ L之间 ,平均为 11.78mg/ L± 3.82 mg/ L ;其中 DOC∶ POC∶ TOC为 0 .72∶ 0 .2 8∶ 1。各种有机碳组分的含量明显高于自然海水中各种有机碳的含量 ;各种有机碳组分的含量在养殖期间总体上呈上升的趋势 ;其变化与 DCOD的变化呈显著线性正相关的关系。  相似文献   

The role of sex change, growth and mortality in Pandalus population dynamics and management     

Fu  Caihong; Quinn  Terrance J.  II; Shirley  Thomas C. 《ICES Journal of Marine Science》2001,58(3):607-621

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声波在水-多孔介质海底界面上的反射与透射     

彭临慧  赵燕鹏  郁高坤 《中国海洋大学学报(自然科学版)》2007,37(4):671-675

声波在海底界面的反射和透射是海底散射、海底混响、海底目标探测的重要问题。利用Biot多孔介质声传播理论对声波在水-多孔海底界面上的反射和透射进行了分析,具体给出反射声波的反射系数,3种透射声波的透射系数以及声能透射系数随入射波入射角和频率(10~40 kHz)的变化关系,分析了各种透射波对透射声能的贡献。多孔海底介质参数分别采用Stoll和Chotiros给出的2组参数进行理论计算。  相似文献   

Multiple sound scattering by densely packed shoals of marine animals     

Andreeva  I. B.; Belousov  A. V. 《ICES Journal of Marine Science》1996,53(2):323-327

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中国对虾支原体的电镜研究     

杨季芳  吴友吕 《海洋学研究》1992,10(4):63-67

1990年7～8月,舟山地区的对虾养殖场暴发了流行性对虾黑鳃褐斑综合症,造成对虾大量死亡。作者根据这一病情,对病虾的主要器官组织进行了电镜观察。结果发现,病虾鳃丝细胞中有支原体感染。寄生部位为鳃呼吸上皮细胞的细胞质和核周腔。支原体多形态,呈近圆形、细长条形不等,无细胞壁,仅有单一膜包围,中心为一电子密度较低的区域。  相似文献   

对虾一种无包涵体杆状病毒病原的PCR检测   总被引：4，自引：0，他引：4  

石拓  孔杰  刘萍  包振民  韩玲玲  徐怀恕 《海洋学报》2000,22(4):96-100

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.  相似文献   

东海虾类的生态群落与区系特征   总被引：6，自引：0，他引：6  

宋海棠 《海洋科学集刊》2002,(44):124-133

有关东海的虾类，刘瑞玉(1959，1963，1964)、董聿茂(1959，1980，1986)等已做过大量的调查研究。近10多年来，由于东海传统的主要经济鱼类资源衰退，捕食虾类的鱼类减少，因此，使虾类生存空间扩大，这有利于虾类资源的繁衍生长，使虾类资源发生量增多，数量增长较快。东海区三省一市近几年虾类产量达到80×104~90×104t，其中浙江省为60×104~70×104t，拖虾作业已成为东海区一大作业方式，对促进海洋捕捞业的发展起重要作用。20世纪80年代中后期，我们开展了东海虾类资源开发调查，尔后又继续与拖虾生产船相结合进行监测调查，积累了一些资料，本文就东海虾类生态群落结构与区系特征进行探讨。  相似文献