海水中低含量铵氮的高灵敏度荧光法测定   总被引：3，自引：0，他引：3  

余翔翔  郭卫东 《海洋科学》2007,31(4):37-41

提出了一种改进的、可测定海水中低含量铵氮的高灵敏度荧光分析方法。其原理是在硼酸缓冲溶液的作用下,海水中的铵氮与邻苯二甲醛(OPA)发生衍生化反应,通过测定生成的荧光产物来确定水体中铵氮的浓度。方法的检测限极低(0.002 5μmol/L),重现性好,且水样用量少(10 mL),适用于海水中溶解有机物的光铵化研究及其他低铵含量水样的测定。  相似文献   

海洋荧光溶解有机物研究进展   总被引：15，自引：1，他引：15  

郭卫东  程远月  吴芳 《海洋通报》2007,26(1):98-106

海洋荧光溶解有机物(FDOM)是海洋有色溶解有机物中可产生荧光的组分,其理化性质对于海洋上层的水色遥感、光化学以及浮游植物的生产力和生态系统结构与功能等都有重要影响。总结了FDOM的3种荧光光谱分析技术及其特点,重点对其两种主要成分(类腐殖质和类蛋白质荧光物质)的荧光特性、分布变化以及来源、分布变化及其去除等进行了全面细致的综述,阐述了研究FDOM的海洋学意义,并对今后有待深入研究的重点问题作了展望。  相似文献   

Utilization of a submersible UV fluorometer for monitoring anthropogenic inputs in the Mediterranean coastal waters     

Marc Tedetti  Catherine Guigue  Madeleine Goutx 《Marine pollution bulletin》2010,60(3):350-2210

We evaluated the performances of a submersible ultraviolet fluorometer (EnviroFlu-HC, TriOS Optical Sensors) dedicated to the real time measurement of polycyclic aromatic hydrocarbons (PAHs) in the aquatic media. We conducted calibration experiments and in situ measurements in the coastal Mediterranean Sea. We found that the EnviroFlu-HC was not strictly specific to PAHs, even though it exhibited the highest sensitivity for phenanthrene, but could response to tryptophan-like material as well, and in a much less extent, to humic substances. The sensor signal showed great spatial and temporal variations in clean and polluted sites, with likely a high contribution of PAHs in the harbors, and a high contribution of tryptophan-like and humic-like materials in the sewage effluent. We conclude that the EnviroFlu-HC is a good tool for monitoring anthropogenic inputs in the coastal waters, although its utilization should be combined to other fluorescence measurements to improve the information about the nature of the aromatic compounds detected.  相似文献   

二阶导数等值点荧光光度法同时测定岩矿中钨和钼     

朱玉伦  裴世桥 《岩矿测试》1993,12(3):171-176

在W(Mo)-茜素磺酸钠荧光体系中,加入一定量乙醇后,可使W、Mo的荧光强度明显增加,Mo比W的灵敏度提高更加显著,为W、Mo在同一体系中进行测定提供了条件。在λ_(em)=603nm时,W的二阶导数荧光值不随含量变化而变化,成为W等值点,而Mo的二阶导数荧光值则有良好的线性关系。同时,λ_(em)=613nm时成为Mo等值点,而W的二阶导数荧光值有良好的线性关系。基于上述事实,提出用等值点法实现在导数荧光光谱仍相互重叠情况下同时测定W和Mo。方法经标准样品分析验证,结果与推荐值吻合。  相似文献   

胰岛素浓度荧光光谱分析     

刘义保 《华东地质学院学报》1998,21(2):167-170

胰岛素是调节细胞代谢、促进细胞生长的重要因子,也是体外细胞无血清培养的必不可少的补充因子．本文测定了胰岛素不同浓度时的荧光发射强度。胰岛素荧光谱能测定胰岛素构象的变化,并能反映胰岛素生物功能的变化。  相似文献   

铁促进海水小球藻油脂积累的动态过程   总被引：6，自引：0，他引：6  

刘志媛  王广策 《海洋科学》2008,32(11):56-59

许多微藻被认为是制备生物油燃料的理想原料,培养液中高浓度的Fe^3＋对海水小球藻（Chlorella vulgaris）的油脂积累有明显的促进作用。采用荧光分光光度法对尼罗红染色后的海水小球藻的油脂积累过程进行了观测,同时测定了铁对小球藻生长和叶绿素含量的影响。结果表明,高铁处理的小球藻出现了两次油脂积累高峰,且第2次脂积累的量显著高于第1次。  相似文献   

Natural occurrence of 4-methyl-phenanthrene in petroleums and recent marine sediments: A new sensitive method to identify and quantify isomers in a series of alkyl—PAH: High resolution spectrofluorimetry at 15 K in n-alkane (Shpolskii effect)     

P. Garrigues  M. Ewald 《Organic Geochemistry》1983,5(2):53-56

HPLC extracts of crude petroleums and of marine recent sediments, containing the monomethyl-phenanthrene series, are examined by high resolution spectrofluorimetry in n-hexane at 15 K (Shpolskii effect). Identification and relative quantification of each of the five isomers is obtained on six natural samples. The distribution of the monomethyl-phenanthrenes and the relative absence of 4-methyl-phenanthrene in petroleums compared to marine sediments may reflect steric inhibitions.  相似文献