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排序方式： 共有92条查询结果，搜索用时 15 毫秒
1.
研究了2-[2-(6-氯苯并噻唑)偶氮]-5-二乙氨基苯甲酸(6-Cl-BTAEB)与Ni2+的显色反应。结果表明:在pH4.5～7.6的HAc-NaAc缓冲溶液中及十二烷基硫酸钠存在下,试剂与Ni2+形成稳定的蓝紫色配合物,组成为n(Ni2+)∶n(6-Cl-BTAEB)=1∶2,最大吸收波长650nm,表观摩尔吸光系数为1.59×105L·mol-1·cm-1,Ni2+浓度在0～400μg/L内符合比尔定律。用铝合金和合金钢中的微量镍验证该分析方法,所得结果与推荐值相符,6次测定的RSD<2%。 相似文献
2.
为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。 相似文献
3.
Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. 相似文献
4.
5.
东昆仑成矿带典型矿床分析 总被引：11，自引：2，他引：11
在综述东昆仑地区成矿地质背景及成矿条件的基础上,通过对肯德可克、驼路沟、督冷沟和大场等典型矿床的剖析,详细论述了矽卡岩型铁多金属矿、海相火山喷气沉积叠加后期改造型钴(金)矿、块状硫化物型铜钴矿和造山型金矿的成矿特征、成矿规律及找矿前景,并结合区域成矿模式、找矿模型和各类矿产、异常的空间分布特征确定出8个成矿远景区,13个找矿靶区。同时指出东昆仑各成矿带的成(找)矿潜力由大到小的依次排序为:昆南成矿带≈阿尼玛卿-北巴颜喀拉成矿带>昆北成矿带>>昆中成矿带。 相似文献
6.
结合课题组前期的研究成果,通过对墙体中不同材料的本构模型、墙体中裂缝、多种材料的联结以及墙体边界条件的研究与探讨,运用通用有限元程序ANSYS对密肋复合墙体进行非线性有限元分析;并在试验结果与有限元模拟的基础上,依据极限平衡理论,建立起来复合墙体的斜截面抗剪极限承载力公式。理论分析与试验研究表明:复合墙体非线性有限元模型能够较好地模拟墙体内力和变形发展的全过程,描述裂缝的形成和扩展,对于实际工程和科学研究具有足够的精度;墙体斜截面抗剪承载力公式与实测值吻合较好,具有一定的精度,满足工程设计需要。 相似文献
7.
正With the rapid increasing demand of metal lithium and its compounds in science and technology,studies on lithium recovery though lithium ion extraction from salt lake brines are blooming bacause more than 60 percent of 相似文献
8.
In this study, two common pollutants （benzo[a]pyrene and methamidophos） in marine environment were tested by comet assay for their inducement of in vivo genotoxic effect to the blood cells of black porgy （ Acanthopagrus schlegeli）. The fish was exposed to 2 μg/L of benzo[a]pyrene （BaP） and methamidophos, and their mixture. The assay was performed on whole blood at 2 h, 5 h, 24 h and 96 h exposure intervals. A significant increase in DNA damage was observed in each treatment with the pollutants. Additive effect of BaP and methamidophos was also found in the experiment. However, the decrease ratios of DNA damage for 5 h and 96 h exposure interals compared with 2 h and 24 h exposure ones, respectively, were noticed. This phenomenon may be explained by the function of repairing process via enzyme cytochrome P450 in the animal. Evidence of the genotoxicity of organophosphorus pesticides （OPs） and polynuclear aromatic hydrocarbons （PAHs） on marine fish are discussed in this paper. 相似文献
9.
10.
Described here is a preliminary and tentative application of a method for fine-classification of stars, selected for studies
of galactic structure. The present investigation is based on about 100 stars within the approximate spectral type range B6-A5,
for which both spectra andUBV photometry have been obtained.
The motivation of the project is the following: For the study of galactic fine-structure it is essential to use as many members
as possible of stellar agglomerations of various type for a statistical treatment of the material. A-type stars are fairly
numerous and reasonably bright, but if all chemically peculiar stars, fast rotators or multiple systems have to be omitted
there is generally too little left for a relevant investigation. Here we perform some experiments in order to find a method
for fine-classification of A stars, both normal and chemically peculiar, within the framework of the M K system.
In this connection it is desirable to reduce the present multitude of CP classifications to a manageable number. It is shown
here that, independenttly of the definition of the degree of peculiarity, there is no sharp borderline separating the CP stars
from the ‘normal’ ones.
Also it is found that spectral classification can on an average be performed with almost the same accuracy for CP stars as
for the ‘normal’ ones provided cases of extreme peculiarity are avoided.
Based on observations collected at European Southern Observatory, La Silla, Chile. 相似文献