排序方式： 共有20条查询结果，搜索用时 15 毫秒
1.
2.
721矿山南铀矿石微生物槽浸的适应性实验研究 总被引：10，自引：0，他引：10
以山南铀矿石为研究对象，探讨了微生物槽浸铀实验过程中有关参数（Fe^3 ,Fe^2 ,pH,Eh,U)的变化规律。在此基础上，评价了山南铀矿石微生物槽浸的适应性。 相似文献
3.
Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil 总被引：1，自引：0，他引：1
Mahalingam Brinda Lakshmi Venkataramagounder Perumal Anandaraj Manickam Velan 《洁净——土壤、空气、水》2013,41(1):86-93
The effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil–slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50–200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X‐100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X‐100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X‐100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites. 相似文献
4.
一系列硫化物矿床氧化带的研究表明，微生物的氧化效果应当引起人们的注意。不同气候区氧化作用对比研究，可以认为干旱地区以化学反应为主，湿润地区则以化学－生物化学联合作用占优势，当微生物参与氧化反应时，传统的硫化物－硫酸盐－氢氧化物－氧化物的反应系统被打乱，而是氧化－还原协调进行，巧妙配合，这是微生物生命活动的需要，是一种动态平衡的演化过程。 相似文献
5.
The inability to detect the presence of viable Helicobacter pylori bacteria in environmental waters has hindered the public health community in assessing the role water may play in the transmission of this pathogen. This work describes a cultural enrichment method coupled with an H. pylori‐specific PCR to identify these bacteria in water. While far from perfected at the present time, this represents an exciting new approach to studying the significance of water as a transmission mechanism for H. pylori. Evidence is presented that indicates culturable H. pylori bacteria were found using this enrichment/PCR method in a local groundwater source. 相似文献
6.
通过建立高效率的微生物未培养DNA提取技术和16SrDNA分析技术,在中国大陆钻探深层岩芯中发现微生物存在的证据。从地下430.00米(S1)与1033.77米(S13)深度岩芯中分别提取到微生物DNA,进行16SrDNAPCR扩增,通过对S13样品DNA中克隆得到的54个16SrDNA的序列测定,初步构建了该深度地下微生物群落的系统进化树,发现了23种不同的微生物序列存在。通过DAPI染色证明在地下1033.77米的微生物数量约为1×104个/克岩石,采用实时定量PCR分析分析,发现了其中两种微生物(CCSD1305与CCSD1307)的数量为6.6×104 /g岩石与2.18×106个/g岩石。 相似文献
7.
This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed. 相似文献
8.
The rational use of pesticides generates an impact which is normally reversed and eliminated by the environment itself. However, the indiscriminate use of pesticides makes its natural degradation rhythm difficult, prolonging their presence in the soil for a great deal of time. Aiming towards a decrease in the environmental impact of pesticides, soil microorganisms capable of degrading pesticides, such as propanil, were investigated. An Enterobacter cloacae strain, isolated from rice field soil, was exposed to the herbicide propanil alone and in a mixture containing also bentazone, clomazone, quinclorac, and 2,4‐D. This bacterium was able to eliminate 100% of the applied propanil in 28 days. Propanil degradation in the 5‐herbicide mixture was much lower than that of individual pesticide degradation. The aeration of the system helped to degrade propanil and its subproduct 3,4‐dichloroaniline much faster. LC with UV detection was used to determine the remaining concentrations of the herbicides and their subproducts. 相似文献
9.
The discovery of the Bozhong 19-6 gas field, the largest integrated condensate gas field in the eastern China in 2018, opened up a new field for the natural gas exploration deep strata in the Bohai Bay Basin, demonstrating there is a great potential for natural gas exploration in oil-type basins. The ethane isotope of the Bozhong 19-6 condensate gas is heavy, showing the characteristics of partial humic gas. In this paper, aimed at the source rocks of the Bozhong 19-6 gas field in the Bohai Bay Basin, the characteristics of the source rocks in the Bozhong 19-6 structural belt were clarified and the reason are explained from impact of microorganism degradation on hydrocarbon generation of source rocks why the condensate oil and gas had heavy carbon isotope and why it showed partial humic characteristics was explored based on the research of parent materials. The following conclusions were obtained: The paleontology of the Bozhong 19-6 structural belt and its surrounding sub-sags is dominated by higher plants, such as angiosperm and gymnosperm. During the formation of source rocks, under the intensive transformation of microorganism, the original sedimentary organic matter such as higher plants was degraded and transformed by defunctionalization. Especially, the transformation of anaerobic microorganisms on source rocks causes the degradation and defunctionalization of a large number of humic products such as higher plants and the increase of hydrogen content. The degradation and transformation of microorganism don’t transform the terrestrial humic organic matter into newly formed “sapropel” hydrocarbons, the source rocks are mixed partial humic source rocks. As a result, hydrogen content incrased and the quality of source rocks was improved, forming the partial humic source rocks dominated by humic amorphous bodies. The partial humic source rocks are the main source rocks in the Bozhong 19-6 gas field, and it is also the internal reason why the isotope of natural gas is heavy. 相似文献
10.
表生地球化学研究十分广泛。表生地质地球化学的研究包括有表生地球化学过程中各种动力学模型的研究，微生物对各种元素地球化学循环的控制作用及机理研究，岩石风化过程中定量指标的研究与应用，稀土元素在表生作用过程中的地球化学行为及有机质在表生矿产特别是金属矿产成矿机制中的作用的研究。上述诸方面的研究近年来已取得了不少新的成果，对表生矿产的形成机制及合理地开发和利用作出了应有的贡献。 相似文献