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A Method To Detect Viable Helicobacter pylori Bacteria in Groundwater
Authors:Debbie Flanigan  Mark Rodgers
Abstract:The inability to detect the presence of viable Helicobacter pylori bacteria in environmental waters has hindered the public health community in assessing the role water may play in the transmission of this pathogen. This work describes a cultural enrichment method coupled with an H. pylori‐specific PCR to identify these bacteria in water. While far from perfected at the present time, this represents an exciting new approach to studying the significance of water as a transmission mechanism for H. pylori. Evidence is presented that indicates culturable H. pylori bacteria were found using this enrichment/PCR method in a local groundwater source.
Keywords:Enrichment  PCR  Detection  Microorganism  Anreicherung  Kultivierung  Anreicherungskultivierung  PCR  Mikroorganismus
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