皱纹盘鲍中国群体和日本群体的自交与杂交F₁ 的RAPD 标记   总被引：47，自引：8，他引：47  

张国范  王继红  赵洪恩  阙华勇  刘晓 《海洋与湖沼》2002,33(5):484-491

利用皱纹盘鲍中国群体和日本群体野生个体的单自交和单正反杂交获得了 4个F1家系 ,其中家系AJC、BCC、CCJ、DJJ分别为日本♀×中国♂、中国♀×中国♂、中国♀×日本♂和日本♀×日本♂组合 ,采用 2 2个引物对上述四个家系及其各自亲本个体的遗传结构进行了RAPD分析。结果表明 ,四个家系的平均杂合度 :AJC为 0 .2 32 9,BCC为 0 .1 667,CCJ为 0 .1 773,DJJ为 0 .1 649;各家系两亲本间遗传距离 :AJC为 0 .2 4 62 ,BCC为 0 .1 70 1 ,CCJ为 0 .2 1 0 9,DJJ为 0 .1 688。各家系子代群体与父母本的遗传距离 :AJC为 0 .1 772和 0 .2 2 2 4 ,BCC为 0 .1 1 81和 0 .1 92 0 ,CCJ为0 .1 1 2 3和 0 .1 691 ,DJJ为 0 .0 694和 0 .1 947。各家系子代个体间的遗传距离AJC为 0 .0 976,BCC为0 .0 95 1 ,CCJ为 0 .0 699,DJJ为 0 .0 682 ,而子代各群体间遗传距离均小于 0 .0 2 2 0。  相似文献   

裙带菜配子体基因工程选择标记的研究          下载免费PDF全文

张亦陈  高江涛  张喆  姜鹏  秦松 《海洋科学》2007,31(12):64-68

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。  相似文献   

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

基于MAPGIS的军事标图系统的设计   总被引：2，自引：0，他引：2  

张剑波  陈宇等 《地球科学》2002,27(3):301-305

军事领域是一个非常特殊的行业，信息的安全性和空间数据的多样性是应用开发的重要问题。在全球信息化日趋高速发展和逐步成熟的今天，军队信息化建设也必须与GIS技术紧密结合，跟上时代前进的步伐。较系统地研究了GIS技术在军事标图绘制中的应用和集成问题，并提供了相应的理论方法和实现系统。在此基础上介绍了以MAPGIS为开发平台的军事标绘系统的实用功能和特点。首先分析了军事信息系统对地理信息数据的要求，并且评述了相关领域的研究现状。然后较为系统地介绍了军事标图系统的总体设计，根据军事标绘对象的数据特征，设计符合要求的应用系统提出了面向对象和无缝交互工具等的设计思想。对军标对象数据模型和操作模型进行了详细地阐述和说明，并将这些模型在系统中进行了实现。最后介绍了军事标图系统的主要模块和功能，探讨了今后GIS研究和发展的方向。  相似文献   

Deformation patterns of upper Quaternary strata and their relation to active tectonics,Po Basin,Italy     

Alessandro Amorosi  Luigi Bruno  Bruno Campo  Bianca Costagli  Wan Hong  Vincenzo Picotti  Stefano Claudio Vaiani 《Sedimentology》2021,68(1):402-424

Despite increased application of subsurface datasets below the limits of seismic resolution, reconstructing near‐surface deformation of shallow key stratigraphic markers beneath modern alluvial and coastal plains through sediment core analysis has received little attention. Highly resolved stratigraphy of Upper Pleistocene to Holocene (Marine Isotope Stage 5e to Marine Isotope Stage 1) alluvial, deltaic and coastal depositional systems across the southern Po Plain, down to 150 m depth, provides an unambiguous documentation on the deformation of previously flat‐lying strata that goes back in time beyond the limits of morphological, historical and palaeoseismic records. Five prominent key horizons, accurately selected on the basis of their sedimentological characteristics and typified for their fossil content, were used as highly effective stratigraphic markers (M1 to M5) that can be tracked for tens of kilometres across the basin. A facies‐controlled approach tied to a robust chronology (102 radiocarbon dates) reveals considerable deformation of laterally extensive nearshore (M1), continental (M2 and M3) and lagoon (M4 and M5) marker beds originally deposited in a horizontal position (M1, M4 and M5). The areas where antiformal geometries are best observed are remarkably coincident with the axes of buried ramp anticlines, across which new seismic images reveal substantially warped stratal geometries of Lower Pleistocene strata. The striking spatial coincidence of fold crests with the epicentres of historic and instrumental seismicity suggests that deformation of marker beds M1 to M5 might reflect, in part at least, syntectonically generated relief and, thus, active tectonism. Precise identification and lateral tracing of chronologically constrained stratigraphic markers in the ¹⁴C time window through combined sedimentological and palaeoecological data may delineate late Quaternary subsurface stratigraphic architecture at an unprecedented level of detail, outlining cryptic stratal geometries at the sub‐seismic scale. This approach is highly reproducible in tectonically active Quaternary depositional systems and can help to assess patterns of active deformation in the subsurface of modern alluvial and coastal plains worldwide.  相似文献   

被动椭圆形标志物应变分析的计算机模拟   总被引：1，自引：0，他引：1  

何绍勋  王焕金 《大地构造与成矿学》1991,15(3):233-242

本文采用计算机数学模拟的方法更深入地探讨了椭圆形标志物应变分析技术(R_f/Φ去应变法、Shimamoto等提出的代数法、调和平均值法和算术平均值法)的适用条件。一共形成了十一组有代表性的应变前数组,四种变形路径:纯剪、单剪、纯剪加单剪和体变。当应变前数组在(-90°,90°)内均匀分布或随机分布时,无论变形路径是哪一种(共轴的还是非共轴的),上述的所有方法都是适用有,且以代数法最佳。当应变前数组具有优选方位(单峰分布)且变形路径为共轴递进变形时,在一定条件下,R_f/Φ法能用来估计应变。经分析发现当应变足够大时,调和平均值法是一种简单快速的应变计算法。  相似文献   

黔西南金地球化学块体资源潜力与找矿方法研究     

何邵麟  程国繁  刘应忠  陈智  胡从亮  牟军  罗明学 《矿物学报》2007,27(3):477-482

根据1：20万区域化探填图数据，从黔西南金的地质地球化学背景分析出发，应用地球化学块体理论和方法预测金矿远景资源量，分析微细浸染型金矿的地质地球化学综合找矿标志和评价准则，探讨金的成矿条件和找矿方向。综合分析认为，黔西南金的成矿条件优越，成矿物质来源丰富，资源潜力巨大，尚有较大找矿前景。  相似文献   

Stratigraphic Correlation of the Malusok Volcanogenic Massive Sulfide Deposits, Southern Mindanao, Philippines     

Cliff L. QUERUBIN  Graciano P. YUMUL Jr. 《Resource Geology》2001,51(2):135-143

Abstract: Two adjacent volcanogenic massive sulfide (VMS) deposits, the Main Malusok and the Malusok Southeast, are delineated within Barangay Tabayo, Siocon, Zamboanga del Norte, Mindanao, Philippines. These deposits comprise massive to semi-massive sulfide lenses representing the down-dip equivalent of oxidized gossans. The massive sulfides have a primary mineral assemblage of pyrite-chalcopyrite-sphalerite with significant amounts of supergene copper in the form of chal-cocite. Owing to structural and metamorphic overprinting combined with intense alteration, primary textures are generally obliterated. Rock types are classified according to dominant mineral assemblages whereas the main lithologic units comprising the Malusok volcanic package are divided based on the position of each unit relative to the mineralized zone. The main lithologic units are designated as the hanging wall, the host, and the footwall sequences. In correlating the stratigraphy of the Main Malusok zone with that of the Malusok Southeast zone, a chlorite/epidote-rich interval located at the base of the hanging wall sequence serves as a distinct stratigraphic marker from which all lithologies are referred to. Comparisons between the stratigraphy of the two areas show that massive to semi-massive sulfide lenses are confined within a single stratigraphic interval representing the favorable horizon for the entire Malusok area. However, differences exist relative to style of mineralization and configuration of the altered interval between the Main Malusok and the Malusok Southeast VMS deposits. Based on characteristics exhibited by each individual deposit, it is inferred that the Main Malusok VMS deposit overlies a feeder zone whereas the Malusok Southeast sulfide lenses represent satellite deposits and transported blocks.  相似文献   

生物质燃烧颗粒物有机示踪化合物的测定和应用   总被引：2，自引：0，他引：2  

张烃刘咸德  董树屏祁辉 Hélène Cachier Magda Claeys 《岩矿测试》2006,25(2):107-113

使用气相色谱-质谱法对2002年7月-2003年7月的北京市大气粗、细颗粒物样品中的左旋葡聚糖等糖类化合物进行了测定。结果表明，左旋葡聚糖主要存在于细颗粒中，可以作为示踪化合物来研究生物质燃烧现象。生物质燃烧对北京市大气颗粒物有较重要的贡献。对应于12％-40％的PM2.5有机碳和10％-33％的PM10有机碳。北京市在2002年10月和11月受到明显的生物质燃烧的影响，可能由于农田秸杆焚烧和秋季落叶焚烧。2003年5月7日颗粒物样品受到直线距离约为1000km以外的内蒙古自治区呼伦贝尔市大兴安岭林区森林大火烟雾的影响。生物质燃烧事件具有突发性，可以长距离传输；生物质作为农村生物燃料的使用其燃烧排放具有经常性和持久性的特征。  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献