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The effects of various salinity fluctuation amplitudes (2, 4, 6 and 8) on the growth, osmolarity, Na+-K+-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied. The results show that weight gain in the salinity fluctuation treatments was better than that in control; in particular, the weight gain of treatments S4 and S0, at 231.8% and 196.3%, respectively, was significantly different (P<0.05). The hemolymph osmolarity of treatments S0, S2, S4, S6 and S8 was 635.4, 630.8, 623.6, 614.4 and 600.3 mOsm/kg, respectively, and decreased with increasing salinity fluctuation amplitude. The level of Na+-K+-ATPase activity in gills of F. chinensis was higher than that in hepatopancreas, but there were no significant differences among all treatments, either in gills or hepatopancreas (P>0.05). The relative level of Hsp70 in treatment S4 was 48.4% and 40.4% higher than control in muscle and eyestalks, respectively, with the highest values being recorded under a salinity fluctuation amplitude of 4. 相似文献
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Genetic parameter estimation for juvenile growth and upper thermal tolerance in turbot (Scophthalmus maximus Linnaeus) 总被引：1，自引：0，他引：1
ZHANG Tianshi KONG Jie LIU Baosuo WANG Qingyin CAO Baoxiang LUAN Sheng WANG Weiji 《海洋学报(英文版)》2014,33(8):106-110
Twenty-six half-sib groups(53 full-sib families) of turbot, Scophthalmus maximus Linnaeus, were obtained by artificial insemination. We measured growth in the offspring(40–50 individuals/family) and subjected them to a thermal tolerance challenge over a period of 34 d. There was no significant difference in daily mortality(range: 0.580%–1.391%) between Days 1–13 during the thermal tolerance challenge. However, daily cumulative mortality increased rapidly between Days 14 and 29, especially on Days 15 and 16(20.232% and 34.377%, respectively). Mortality was highest on Day 16(14.145%). We estimated the genetic parameters using the average information restricted maximum likelihood method. We used a likelihood ratio test to evaluate the significance of effects in models with and without identity as an effect, and compared the final log-likelihoods(maximum log L). Lastly, we estimated phenotypic and genetic correlation between the upper thermal tolerance limit(UTT) and body weight(BW). In this study, the positive phenotypic correlation was low between UTT and BW(0.093±0.029). The genetic correlation between UTT and BW was negative(-0.044±0.239). The heritability for upper thermal tolerance was low(0.087±0.032), which is of approximately moderate heritability. The heritability for body weight was high(0.303±0.074). Our results suggest there is significant potential for improvement in the culture of turbot by selective breeding. 相似文献
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“少年海洋学校”成立后，由于我国还没有一套集知识性、科学性、系统性、趣味性于一体的适合少年儿童使用的海洋科普教材，给上海洋科普活动课的教师带来了极大的不便。为此，学校领导大胆地提出了由学校教师自己创编《少年海洋科普活动教材》的设想。 相似文献
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同治十三年(1874年)十二月初五夜里,像往常一样,不满四周岁的我,早早地就在奶娘的故事中进入梦乡。待到天蒙蒙亮的时候,剧烈的晃动把我惊醒。眼前的一切都变了。这里不是醇王府,但比家里豪华得多。一个老太监把睡眼惺忪的我从怀抱中放了下来,让我在地上跪倒磕头。偷眼旁观,很多大臣也都在下跪,其中有一个熟悉的身影——父亲。我想叫一声,但整个屋子里一片肃杀,我不敢。磕头之后,抬起头来,面前的宝座空无一人,宝座后面的垂帘里,端坐着两个年逾四旬的女人。其中一个女人一抬手,我就在糊里糊涂中被一个太监抱到了这个宝座上。只见父亲和所有的大臣都向我磕头,山呼万岁。30多年过去了,现在想起来还觉得可笑。从那一刻开始,我就成为这个帝国的君主。但作为专属于我的年号,"光绪"却并没有给我带来多少好运,反而像梦魇一样折磨了我30多年。 相似文献
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The effects of a diet containing Hanseniaspora opuntiae C21 on growth and digestive enzyme activity were estimated in juvenile Apostichopus japonicus. Groups of sea cucumbers were fed diets containing H. opuntiae C21 at 0(control), 10 4, 10 5, and 10 6 CFU(colony-forming units)/g feed. Results showed that after 45 d the specific growth rate(SGR) of sea cucumbers fed a C21-supplemented diet at 10 4 CFU/g feed was significantly higher than that of the control( P 0.05). Intestinal trypsin and lipase activities were significantly enhanced by C21 administration at 10 4 and 10 5 CFU/g feed compared with the control( P 0.05). After feeding for 23–42 d, C21 was demonstrated by denaturing gradient gel electrophoresis to be present in the intestine of sea cucumbers. In addition, after feeding the C21-supplemented diets for 15 d, the sea cucumbers were switched to an unsupplemented diet and C21 was confirmed to be capable of colonizing the intestine for at least 31 d after cessation of feeding. In conclusion, C21 was shown to successfully colonize the intestine of juvenile A. japonicus via dietary supplementation, and improve growth and digestive enzyme activity. 相似文献
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A study on the nonspecific immunity of Litopenaeus vannamei ever inhabiting freshwater and seawater was carried out at different molt stages by comparing their total hemocyte count(THC) and respiratory burst(RB) and activity of phenol oxidase(PO), nitric oxide synthase(NOS) and lysozyme(LY). Two-way ANOVA showed that salinity and molt stage independently affected THC and RB and the activity of PO, NOS and LY of juvenile L. vannamei significantly(P 0.05). The THC and RB and the activity of NOS gradually increased from the post-molt stages(A and B) to the pre-molt stages(D0–D3), which were common in shrimps inhabiting freshwater and seawater. The activity of PO peaked at the inter-molt stage(C), and touched the lowest at the post-molt stage in freshwater and pre-molt stage in seawater. The activity of LY was stable over the molt cycle. The RB and the activity of PO, NOS and LY of juvenile L. vannamei were significantly lower in freshwater than in seawater; whereas THC was significantly higher in freshwater than in seawater(P 0.05). It was concluded that the post-molt stage(especially stage A) was critical to shrimp culture, which should be intensively attended when L. vannamei was cultured in freshwater. 相似文献