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为了了解沅水五强溪大坝阻隔对光泽黄颡鱼可能造成的遗传多样性影响,采用AFLP分子标记技术,对沅水五强溪水库库区和沅水下游两个光泽黄颡鱼野生群体进行了遗传多样性分析。9对选择性引物在两个光泽黄颡鱼群体中,共扩增出1243个位点,多态位点1091个,多态位点比率为87.77%。其中库区和沅水下游群体多态位点比率分别为85.41%、83.25%,观测等位基因数分别为1.854±0.592、1.803±0.622,有效等位基因数分别为1.597±0.381、1.574±0.377,Nei’s多样性指数分别为0.220±0.211、0.207±0.195,Shannon’s信息指数分别为0.330±0.305、0.302±0.338;两个群体内的平均遗传距离分别为0.124±0.072、0.119±0.057,群体间的平均遗传距离为0.127±0.100。两个群体的遗传参数比较不存在显著差异(P0.05)。聚类分析显示,UPGMA系统树没有依据群体而形成明显的两大分支。以上结果表明,五强溪水库库区和沅水下游两个光泽黄颡鱼群体的遗传多样性丰富,光泽黄颡鱼种群遗传结构对水库大坝阻隔的响应不明显。 相似文献
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利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。 相似文献
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AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam(Ruditapes philippinarum) in China 总被引:7,自引:0,他引:7
LIU Xiangquan BAO Zhenmin HU Jingjie WANG Shi ZHAN Aibin LIU Hui FANG Jianguang WANG Rucai 《海洋学报(英文版)》2007,26(1):150-158
1 IntroductionManila clam (Ruditapes philippinarum) is aworldwide distributed species. In China, it is foundfrom the northern to southern seashores (Wang etal., 1993; Zhuang, 2001). This species is eura-line with the salinity tolerance ranging from 20 to4… 相似文献
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橙色莫桑比克罗非鱼和荷那龙罗非鱼的AFLP分析 总被引:6,自引:0,他引:6
运用AFLP分子标记技术检测了橙色莫桑比克罗非鱼(Oreochromis mossambicus)和荷那龙罗非鱼(Oreochromis hornorum)的种群遗传多样性。提取2种鱼的基因组DNA,经EcoR Ⅰ和Mse Ⅰ限制性内切酶酶切并加接头,从16对选扩引物组合中选出3对扩增结果较好的引物进行PCR扩增。扩增产物经PAGE胶电泳、银染,检测其多态性。3对引物在2种罗非鱼的40个个体中共扩增出94条带,其中多态性条带76条,占总扩增条带的80.9%,2个群体的多态性条带比例分别为71.6%和52.4%。根据Nei氏(1978)统计分析得出橙色莫桑比克罗非鱼种群内遗传相似系数为0.765。荷那龙罗非鱼的为0,821,表明2个罗非鱼种群均具有较高的遗传多样性,橙色莫桑比克罗非鱼种群内遗传多样性相对更高些。2种罗非鱼的种间遗传距离为0.279,推测该杂交组合可能具有杂种优势。 相似文献
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LI Qi XU Yanhong YU Ruihai KIJIMA Akihiro 《中国海洋大学学报(英文版)》2007,6(3):259-267
A genetic linkage map of Pacific abalone (Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations, a total of 455 markers (225 from the female parent and 230 from the male parent) segregated in a 1 : 1 ratio, corresponding to DNA polymorphism: heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups, equivalent to the H. discus hannai haploid chromosome number, and spanning a total length of 2031.4 cM, with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups, spanning a total length of 2273.4cM, with an average spacing of 12.9cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male, on the basis of two estimates of genome length. Fifty-two markers (11.4%) remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs. 相似文献
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利用AFLP技术对山东沿海菲律宾蛤仔(Ruditapes philippinarum)4个野生群体(乳山、无棣、牟平、即墨)进行了遗传多样性分析。采用6对引物组合对4个群体进行扩增,共得到356个位点,乳山群体、无棣群体、牟平群体和即墨群体的多态位点比例依次为74.40%、70.50%、72.80%、74.70%,具有较高的遗传多样性水平。其中,即墨群体多态性比例最高,无棣群体多态性比例最低。聚类分析表明,各群体间的遗传距离为0.0263~0.0448,乳山群体和无棣群体间的遗传距离最近(0.0263),两群体首先聚在一起,随后与牟平群体和即墨群体聚在一起。 相似文献
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Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis 总被引:1,自引:0,他引:1
Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes. 相似文献