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Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community. 相似文献
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冬季气候变暖对山西省冬小麦可种植区的影响 总被引：4，自引：0，他引：4
基于山西省境内较为均匀分布的70 个地面气象观测站1970-2012 年冬季逐日气温资料,采用线性倾向估计法分析了负积温、最冷月平均气温和年极端最低气温的变化特征,采用累积距平法确定其突变点,以突变点为界分为前后2 个时间段,依据前后时间段等值线的变化分析冬季气候变暖对山西省冬小麦可种植区的影响。结果表明：山西省负积温呈现显著减少趋势（通过了α=0.01 的显著检验）,最冷月平均气温和年极端最低气温呈现不显著升高趋势;突变后,负积温平均减少了103.4℃,最冷月平均气温和年极端最低气温分别升高了0.8℃和0.7℃;在3 个指标中,决定山西省冬小麦能否种植的关键因子是负积温和年极端最低气温,最冷月平均气温的影响较小;冬季气候变暖后,平均状况下,冬小麦可种植区域面积扩大了约2.9×106 hm2,扩大52%,80%保证率下,冬小麦种植面积扩大了约2.3×106 hm2,扩大79%。 相似文献
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利用我国南方稻区214站1961—2009年逐日气象资料,研究气候变化对南方水稻可种植区的影响。研究结果显示：气候变暖使南方稻区活动积温（日平均气温≥10℃）明显增加,49年增加了324.4℃?d。同时水稻生长季长度也明显延长,49年延长了17.9 d。双季稻可种植区北界明显北移,三季稻可种植区北界略有北移,20世纪60—80年代,双季稻可种植区仅限于长江以南地区,但21世纪初以来的10年双季稻可种植区北界移到长江以北,即向北推移近300 km,从而使新增双季稻可种植区扩展到四川东北部、贵州东部、重庆、湖北大部、安徽中部以及江苏南部。 相似文献
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不同海拔表层土壤微生物数量消长的机理 总被引：3，自引：3，他引：0
以乌鲁木齐河源区不同海拔表层土壤样品为研究材料,利用荧光显微计数技术、寡营养恢复培养技术等,研究了微生物的数量与土壤理化参数和植被类型的关联度.结果表明:表层土壤中可培养细菌数与表土含水量、总C、总N和pH值相关性不显著,与相应的植被类型明显相关,可培养细菌数从大到小所对应的植被为:苔草嵩草芨芨草针阔叶混交林云杉车前草山莓草;在相同植被类型下,可培养细菌数量表现出随海拔升高而降低的趋势.植被类型是影响土壤可培养细菌数量的主要因素,但海拔变化对可培养细菌数量的影响也不可忽略. 相似文献
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Marine sponge Tedania anhelans distributes throughout the intertidal zone of Fujian, southeastern China, and is a potential source of natural bioactive products. The sponge harbors a large number of bacterial groups that have been identified using various techniques, including fluorescent in situ hybridization (FISH). Fractionation of dissociated sponge allowed isolation of 25 bacterial species. Based on 16S rRNA gene sequencing, phylogenetic analysis attributed most of these eubacteria to α-Proteobacteria, γ-Proteobacteria, Cytophaga/Flavobacterium/Bacteroidetes (CFB group), and the family Bacillaceae of Gram-positive bacteria. In sequence similarity, five putatively novel species were identified with less than 98% similarity to other strains in the NCBI database. Tests for antimicrobial activities were performed against Gram-positive bacteria, Gram-negative bacteria, fungi, antitumor indicators Escherichia coli 343/591 (with DNA repair deficiency), regular E. coli 343/636 (with different DNA repair capacity), and 10 bacterial isolates exhibited inhibitory bioactivities. Among these strains, three isolates were detected involving function gene NRPS-A domains, which were most closely related to the amino acid sequences of linear gramicidin synthetase and pyoverdine synthetase. These results contribute to our knowledge of the microbes associated with marine sponges and further reveal novel bacterial resources for the screening of bioactive marine natural products. 相似文献
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以青藏高原昆仑山垭口不同深度土壤样品为研究对象,研究了可培养细菌数量及多样性。结果表明：可培养细菌数量与多样性在一定程度上均与土壤深度呈负相关关系。可培养细菌数量以表层土壤最多,而细菌多样性最低。基于16S rDNA基因序列分析共发现了6个门,18个属,21种细菌,其中表层土壤Arthrobacter siccitolerans为绝对优势种,比例达95%;冻土区（0~82.15m）之间不同土样Mycetocola miduiensis菌株所占比例较大;而冻土层以下没有明显的优势菌。冗余分析（RDA）显示：可培养细菌数量主要受土壤有机碳影响,土壤含水量则是影响细菌多样性的主要因素。 相似文献
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Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter. 相似文献
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坡度是最基本的地形因子之一。本文介绍了基于DEM的坡度计算方法,对坡度分级统计中的误差来源分类进行分析,并实验分析其DEM精度,以及各误差因素对坡度统计计算的影响。 相似文献