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The changes of three components in coelomic fluid of Urechis unicinctus exposed to different concentrations of sulfide 总被引：4，自引：0，他引：4
The changes in heme （associated with hemoglobin）, hemoglobin and hematin in the coelomic fluid of marine worm, Urechis unicinctus, exposed to different concentrations of sulfide, were investigated using biochemical techniques. When exposed to different sulfide concentrations for up to 96 h, the relative amounts of the three components changed in a regular pattern suggesting that the coelomocytes play an important role in the worm＇s tolerance to sulfide. The possible roles of heme compounds in sulfide tolerance of this species are discussed on the basis of our experimental data. 相似文献
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泥蚶血红蛋白(Tg-HbⅡ)除了具有携氧功能外,还具有过氧化物酶活性和抗菌活性。本文采用分光光度法、光谱法和分子对接等技术研究了十二烷基硫酸钠(SDS)对Tg-HbⅡ的结构、过氧化物酶活性及抗菌活性的影响,以探讨Tg-HbⅡ具有过氧化物酶活性及抗菌活性的结构基础。研究结果显示,SDS的疏水烷基长链可嵌入到Tg-HbⅡ血红素口袋内部,与近端His104形成氢键,断裂血红素铁与His104的配位键,使Tg-HbⅡ的Soret带吸收峰降低并发生位移;此外,SDS还可与血红素口袋中的氨基酸形成疏水相互作用,改变血红素口袋原有结构,使得部分疏水氨基酸暴露,导致外源荧光强度增强,最大发射波长红移。SDS可以抑制Tg-HbⅡ的过氧化物酶活性,当SDS浓度为2 mmol•L–1时,Tg-HbⅡ的酶活性仅为原来的20%,在琼脂扩散实验中失去对枯草芽孢杆菌的抗菌活性。以上结果表明,SDS通过破坏血红素口袋的内部结构抑制Tg-HbⅡ的过氧化物酶活性,使其失去抗菌活性,血红素疏水口袋是Tg-HbⅡ具有过氧化物酶活性和抗菌活性的关键结构。本研究为进一步研究Tg-HbⅡ的抗菌机理奠定基础。 相似文献
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用广东沿海盛产的半叶马尾藻Ｓａｒｇａｓｓｕｍｈｅｍｉｐｈｙｌｌｕｍ为原料提取多糖，与Ｆｅ（３＋），Ｚｎ（２＋）等微量元素进行反应，形成能溶于水的海藻多糖配合物。分别以剂量为３００ｍｇ·ｋｇ（－１）的海藻多糖配合物饲喂ＮＩＨ系小鼠７－１５ｄ。结果发现，该多糖配合物能提高小鼠的血红蛋白和红细胞数量。其中，以Ｆｅ（３＋）海藻多糖配合物效果最显著。观察各种免疫指标，发现海藻多糖和海藻多糖微量元素配合物都能提高小鼠的免疫指标。海藻多糖微量元素配合物的效果比海藻多糖好。结果提示，微量元素参与了机体的免疫活动，并提高了免疫活性。 相似文献
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The changes in heme (associated with hemoglobin), hemoglobin and hematin in the coelomic fluid of marine worm,Urechis unicinctus, exposed to different concentrations of sulfide, were investigated using biochemical techniques. When exposed to different
sulfide concentrations for up to 96 h, the relative amounts of the three components changed in a regular pattern suggesting
that the coelomocytes play an important role in the worm's tolerance to sulfide. The possible roles of heme compounds in sulfide
tolerance of this species are discussed on the basis of our experimental data.
This research was supported by the NSFC-KOSEF scientific cooperation program and NSFC (No. 30271039) 相似文献
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A research is conducted on the relationship between the normal reference value of 20,475 examples of hemoglobin of young women and five geographical factors in 208 areas in China, the normal reference value is determined by the hemoglobincyanide method. It is found that the correlation of geographical factors and the normal reference value of hemoglobin of young women are quite significant (F=142.81). By using the method of multiple linear regression analysis, one regression equation is inferred. If geographical values are obtained in some areas, the normal reference value of hemoglobin of young women of this area can be reckoned by using the regression equation. Furthermore, depending on the geographical factors, China can be divided into six regions: Qinghai-Tibet (Qingzang) Plateau, Southwest, Northwest, Southeast, North and Northeast China. 相似文献
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葛淼 《地理学报(英文版)》2003,13(3):331-338
A research is conducted on the relationship between the normal reference value of 20,475 examples of hemoglobin of young women and five geographical factors in 208 areas in China, the normal reference value is determined by the hemoglobincyanide method. It is found that the correlation of geographical factors and the normal reference value of hemoglobin of young women are quite significant (F=142.81). By using the method of multiple linear regression analysis, one regression equation is inferred. If geographical values are obtained in some areas, the normal reference value of hemoglobin of young women of this area can be reckoned by using the regression equation. Furthermore, depending on the geographical factors, China can be divided into six regions:Oinghai-Tibet (Qingzang) Plateau. Southwest. Northwest. Southeast. North and Northeast China. 相似文献
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本文利用Cu2+处理泥蚶血红蛋白（Tg-HbⅡ），分别检测Cu2+对Tg-HbⅡ的过氧化物酶活性及其结构的影响，同时检测Pro对Cu2+作用下Tg-HbⅡ的保护作用。结果表明:随着Cu2+浓度的增加，Tg-HbⅡ的过氧化物酶活性逐渐丧失，Cu2+抑制Tg-HbⅡ的过氧化物酶活性类型属于非竞争-竞争混合型抑制，其失活速率具有浓度依赖性，失活过程呈单相变化，表现为一级反应动力学，并且不同浓度Cu2+能影响Tg-HbⅡ的三级结构变化；另外，Pro对Tg-HbⅡ的空间结构及其过氧化物酶活性具有保护作用，能阻止Tg-HbⅡ的聚沉、恢复Tg-HbⅡ的结构与过氧化物酶活性。 相似文献
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Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study, we introduced a Vitreoscilla hemoglobin gene （vgb） into Chlorella vulgaris by Agrobacterium tumefaciens-mediated transformation （ATMT）. PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L, which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants, which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris, with applications to lutein production from Chlorella during fermentation. 相似文献
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泥蚶血红蛋白的制备及其抗菌活性研究 总被引：3，自引：2，他引：1
利用凝胶层析技术从泥蚶血细胞裂解物中分离纯化得到两种血红蛋白,回收率为74.3%,并通过Tricine-SDS-PAGE和质谱技术鉴定为Tg-HbⅠ(同源二聚体)和Tg-HbⅡ(异源四聚体)。分别通过琼脂扩散法和分光光度法测其抗菌活性和过氧化物酶活性,结果发现,两种Tg-Hb对大肠杆菌和恶臭假单胞杆菌均有抗菌活性,Tg-HbⅡ对于恶臭假单胞菌和大肠杆菌的MIC值分别为0.063mg/mL和0.13mg/mL,Tg-HbⅠ对两种菌的MIC均为0.048mg/mL;两种Tg-Hb均具有过氧化物酶活性,能催化愈创木酚、邻苯二酚、对苯二酚、苯酚、左旋多巴等多种酚类物质。在Tg-Hb中添加GSH后,两种Tg-Hb均不再对恶臭假单胞杆菌具有抗菌活性。可见,两种Tg-Hb对恶臭假单胞杆菌等的抗菌活性均是通过其过氧化物酶活性发挥作用的。 相似文献
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