| Cu2+对泥蚶血红蛋白(Tg-HbⅡ)的过氧化物酶活性与结构的影响 |
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| 引用本文： | 张顺琴,王素芳,陈梦玫,林志华.Cu2+对泥蚶血红蛋白(Tg-HbⅡ)的过氧化物酶活性与结构的影响[J].海洋学报,2018,40(1):106-114. |
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| 作者姓名： | 张顺琴 王素芳 陈梦玫 林志华 |
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| 作者单位： | 浙江万里学院 生物与环境学院水产种质资源高效利用技术省重点实验室, 浙江 宁波 315100 |
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| 基金项目： | 浙江省基金项目（LQ15C190001）；浙江省重中之重生物工程学科资助（CX2016009）。 |
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| 摘 要： | 本文利用Cu2+处理泥蚶血红蛋白（Tg-HbⅡ），分别检测Cu2+对Tg-HbⅡ的过氧化物酶活性及其结构的影响，同时检测Pro对Cu2+作用下Tg-HbⅡ的保护作用。结果表明:随着Cu2+浓度的增加，Tg-HbⅡ的过氧化物酶活性逐渐丧失，Cu2+抑制Tg-HbⅡ的过氧化物酶活性类型属于非竞争-竞争混合型抑制，其失活速率具有浓度依赖性，失活过程呈单相变化，表现为一级反应动力学，并且不同浓度Cu2+能影响Tg-HbⅡ的三级结构变化；另外，Pro对Tg-HbⅡ的空间结构及其过氧化物酶活性具有保护作用，能阻止Tg-HbⅡ的聚沉、恢复Tg-HbⅡ的结构与过氧化物酶活性。
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| 关 键 词： | Cu2+ 泥蚶 血红蛋白 过氧化物酶 结构 |
| 收稿时间： | 2017/6/26 0:00:00 |
Effects of Cu2+ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ) |
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| Zhang Shunqin,Wang Sufang,Chen Mengmei and Lin Zhihua.Effects of Cu2+ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)[J].Acta Oceanologica Sinica (in Chinese),2018,40(1):106-114. |
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| Authors: | Zhang Shunqin Wang Sufang Chen Mengmei and Lin Zhihua |
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| Institution: | Biology and Environment Key Laboratory of Aquatic Germplasm Resource, Zhejiang Wanli University, Ningbo 315100, China |
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| Abstract: | We studied the effects of Cu2+ on the peroxidase activity and structure ofthe Tegillarca granosa hemoglobin (Tg-HbⅡ),and the protective action of Pro to the Tg-HbⅡthat has been treated by Cu2+. The results showed that the peroxidase activity of Tg-HbⅡ was gradually decreased with the increase of Cu2+ concentration, and the inhibition of Cu2+ to Tg-HbⅡperoxidase activity belonged to noncompetitive-competitive mixed inhibition type.The inactivation rate was in a concentration-dependent manner, and the inactivation process showed a single-phase change, which showed a first-order reaction kinetics. The results also indicated that different concentrations of Cu2+ could affect the tertiary structure of Tg-HbⅡ. In addition, Pro could protect the spatial structure of Tg-HbⅡ and restore its peroxidase activity destroyed by Cu2+, which successfully blocked Tg-HbⅡaggregation, recoverying the conformation and activity of Tg-HbⅡ. |
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| Keywords: | Cu2+ Tegillarca granosa hemoglobin peroxidase structure |
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