Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye     

冯金华  于华华  李翠萍  邢荣娥  刘松  王琳  蔡圣宝  李鹏程 《中国海洋湖沼学报》2009,27(4):869-874

The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.  相似文献   

厦门港几种水母的刺胞研究   总被引：1，自引：0，他引：1  

王文樵  许振祖 《台湾海峡》1990,9(2):161-165

本文以厦门港的几种水母为材料在国内首次研究了它们的刺胞。文中还讨论、总结了各水母类的刺胞、探讨了引起刺胞释放的刺激及刺胞的功能。  相似文献