Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye |
| |
| Authors: | FENG Jinhua YU Huahua LI Cuiping XING Ronge LIU Song WANG Lin CAI Shengbao LI Pengcheng |
| |
| Institution: | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China [2]Graduate School, Chinese Academy of Sciences, Beijing, 100039, China |
| |
| Abstract: | The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of
nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses
demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was
the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl
buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration
and the HU50 against chicken erythrocytes was 0.91 μg/mL. |
| |
| Keywords: | nematocyst jellyfish venom Rhopilema esculentum hemolytic activity |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ |
|
