| Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population |
| |
| 作者姓名： | ZHOU Hantao GAO Yuanyuan CHEN Xu YE Fan LIAN Yuwu |
| |
| 作者单位： | ZHOU Hantao1，2*，GAO Yuanyuan1，CHEN Xu1，YE Fan1，LIAN Yuwu1 1. School of Life Sciences，Xiamen University，Xiamen 361005，China2.Key Laboratory of the Ministry of Education for Cell Biological Tum or Cell Engineering，Xiamen Uinversity，Xiamen 361005，China |
| |
| 摘 要： | 1 Introduction Branchiostom a belcheri G ray (am phioxus) is aprecious m arine species listed in the nationalprotectedanim als ofsecond class (H uang etal., 1999). Itbe-longs to the subphylum cephalochordata ofthe phylumvertebrata. A m phioxus is the ancestor of vertebrates5×108 a ago, and is a typical transitionalsam ple ofevolution from invertebrates to vertebrates. The re-search of am phioxus,therefore,is ofgreatim portanceboth for anim al taxonom y and organic evolution(N ozakiand G or…
|
| 关 键 词： | Branchiostoma belcheri Gray genetic diversity molecular markers ISSRs RAPDs genetic conservation |
| 收稿时间： | 2004/4/11 0:00:00 |
| 修稿时间： | 2004/12/2 0:00:00 |
Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population |
| |
| ZHOU Hantao,GAO Yuanyuan,CHEN Xu,YE Fan,LIAN Yuwu.Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population[J].Acta Oceanologica Sinica,2005,24(2):102-112. |
| |
| Authors: | ZHOU Hantao GAO Yuanyuan CHEN Xu YE Fan and LIAN Yuwu |
| |
| Institution: | 1.School of Life Sciences, Xiamen University, Xiamen 361005, China;Key Laboratory of the Ministry of Education for Cell Biological Tum or Cell Engineering, Xiamen Uinversity, Xiamen 361005, China2.School of Life Sciences, Xiamen University, Xiamen 361005, China |
| |
| Abstract: | Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates.Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon' s index of genetic diversity was used to partition the diversity of the animals among these 3sea areas, and the results showed that the indices in populations ofXiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374,respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward. |
| |
| Keywords: | Branchiostoma belcheri Gray genetic diversity molecular markers ISSRs RAPDs genetic conservation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《海洋学报(英文版)》下载免费的PDF全文 |
