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| 湖泊底泥中微囊藻DNA的分子检测 | |
| 引用本文： | 徐瑶,李仁辉,王国祥,余博识,杨文斌.湖泊底泥中微囊藻DNA的分子检测[J].湖泊科学,2007,19(4):492-496. |
| 作者姓名： | 徐瑶 李仁辉 王国祥 余博识 杨文斌 |
| 作者单位： | 1. 南京师范大学地理科学学院,南京,210097;中国科学院水生生物研究所,武汉,430072 2. 中国科学院水生生物研究所,武汉,430072 3. 南京师范大学地理科学学院,南京,210097 4. 中国科学院水生生物研究所,武汉,430072;西南林学院资源学院,昆明,650224 |
| 基金项目： | 中国科学院前沿领域研究基金 , 国家高技术研究发展计划(863计划) , 国家"211"工程重点项目 , 江苏省重点科技基金 |
| 摘 要： | 通过改进裂解温度和延长裂解时间并增加苯酚/氯仿洗脱次数的DNA提取方法获得南京玄武湖底泥中的DNA,通过PCR法来扩增微囊藻的16SrRNA基因.结果表明在所有采样点中均得到微囊藻基因组DNA,并且纯度较高,OD260/OD280均高于1.54,最高值达到1.89.PCR的扩增结果显示所有样点的DNA都得到212 bp大小的微囊藻16SrRNA基因片断,表明这种方法可以有效的从底泥中提取微囊藻的DNA,从而为研究底泥微囊藻生理生态及其越冬、上浮、形成水华的机理提供更有利的方法. |
| 关 键 词： | 底泥 微囊藻 DNA 水华 蓝藻 湖泊底泥 微囊藻 分子检测 Microcystis detection Molecular Cyanobacteria amplification 机理 水华 上浮 越冬 生理生态 研究 基因片断 大小 显示 基因组 采样点 结果 |
| 收稿时间： | 2006-08-06 |
| 修稿时间： | 2006-08-062006-10-19 |
Molecular detection of Microcystis (Cyanobacteria) from sediments by DNA amplification |
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| XU Yao,LI Renhui,WANG Guoxiang,YU Boshi and YANG Wenbin.Molecular detection of Microcystis (Cyanobacteria) from sediments by DNA amplification[J].Journal of Lake Science,2007,19(4):492-496. | |
| Authors: | XU Yao LI Renhui WANG Guoxiang YU Boshi and YANG Wenbin |
| Institution: | College of Geographical Sciencey Nanjing Normal University, Nanjing 210097, P. R. China;Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, P. R. China,Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, P. R. China,College of Geographical Sciencey Nanjing Normal University, Nanjing 210097, P. R. China,Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, P. R. China;School of Nature Resources, Southwest Forestry University, Kunming 650224, P. R. China and College of Geographical Sciencey Nanjing Normal University, Nanjing 210097, P. R. China |
| Abstract: | A method for DNA extraction from sediment samples of Lake Xuanwu, Nanjing, China was developed, and purification and efficience of extracted DNAs were evaluated through ultraviolet spectrum OD260/OD2s0 ratios and PCR amplification of 16S rDNA specific for Microcystis. The results showed that sediment samples from all sites in the study obtained quite purified genomic DNAs with OD260/OD280 ratios from 1.54 to 1.89, and Microcystis 16S rRNA gene fragments have been detected in all sampling sites, which indicating this method can be used for molecular detection of Microcystis cells in sediments. |
| Keywords: | Sediment Microcystis DNA waterbloom cyanobacteria |
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