| 日本对虾血清三类免疫球蛋白样物质的研究 |
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| 引用本文： | 章跃陵,彭宣宪,王三英.日本对虾血清三类免疫球蛋白样物质的研究[J].海洋科学,2001,25(5):37-41. |
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| 作者姓名： | 章跃陵 彭宣宪 王三英 |
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| 作者单位： | 厦门大学生命科学学院细胞生物学与 |
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| 基金项目： | 国家海洋局第三海洋研究所海洋生物工程重点实验室
开放课题基金!资助项目 |
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| 摘 要： | 采用单向免疫扩散法（SRID）和免疫酥斑点法（Dot-ELISA)对日本虾（Penaeus japonicus)血清中类IgM，IgG,IgA样物质进行了系统的研究分析。结果发现，虾血清中存在能与羊抗人IgM,IgG,IgA发生结合且能被特异性阻断试验确证的物质。文中还对12例对虾血清类IgM,IgG,IgA样物质的含量进行了检测。结果显示，虾血清中确实存在与人免疫球蛋白（Ig）特异性抗原决定簇极为相似的物质，它的存在对研究虾的抗病机制具有重要意义。
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| 关 键 词： | 日本对虾 免疫球蛋白 单向免疫扩散 免疫酶斑点法 血清 抗病机制 |
| 收稿时间： | 2000/7/25 0:00:00 |
| 修稿时间： | 2000年7月25日 |
STUDY ON THREE CLASSES OF IMMUNOGLOBULINLIKE COMPONENTS IN Penaeus japonicus |
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| ZHANG Yue-ling,PENG Xuan-xian,WANG San-ying.STUDY ON THREE CLASSES OF IMMUNOGLOBULINLIKE COMPONENTS IN Penaeus japonicus[J].Marine Sciences,2001,25(5):37-41. |
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| Authors: | ZHANG Yue-ling PENG Xuan-xian WANG San-ying |
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| Abstract: | Both methods of single radial immunodiffusion(SRID) and dot-EII SA were used to characterize three kinds of immunnoglobulin-like proteins (IgM, IgG and IgA) in serum of Penaeus japonicus. The results showed that there were components reacted with anti - human μ, γ or achain in the serum, which could be blocked by specific tests. Furthermore, the IgM- like, IgG - like and IgA - like components were measured in 12 cases of Penaeus japonicus. These findings suggest that there were materials with similar isotopes in serum from both the shrimp and human. And Ig - like proteins in the
shrimps were important to understand their disease-free mechanism. |
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| Keywords: | Penaeus japonicus Immunoglobulin Single radial immunodiffusion Dot ELISA |
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