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| 大黄鱼过氧化物酶Ⅳ在人胚肾细胞中的表达与抗氧化活性测定 | |
| 引用本文： | 王贤慧,母尹楠,聂品,陈新华,敖敬群.大黄鱼过氧化物酶Ⅳ在人胚肾细胞中的表达与抗氧化活性测定[J].台湾海峡,2014(4):492-498. |
| 作者姓名： | 王贤慧 母尹楠 聂品 陈新华 敖敬群 |
| 作者单位： | 1. 集美大学水产学院，福建 厦门361021; 国家海洋局第三海洋研究所 国家海洋局海洋生物遗传资源重点实验室，福建 厦门361005 2. 国家海洋局第三海洋研究所 国家海洋局海洋生物遗传资源重点实验室,福建 厦门,361005 3. 集美大学水产学院,福建 厦门,361021 |
| 基金项目： | 国家自然科学基金资助项目(31372556)；厦门南方海洋研究中心资助项目(13GZP002NF08)；宁波大学水产浙江省重中之重一级学科开放基金资助项目 |
| 摘 要： | 为了研究大黄鱼（Pseudosciana crocea）peroxiredoxin Ⅳ（Lyc-Prx Ⅳ）在细胞内的抗氧化功能,构建了表达大黄鱼Prx Ⅳ的重组质粒p CMV-Lyc-Prx Ⅳ,瞬时转染人胚肾细胞（HEK-293T）,利用Western blotting方法检测转染后的细胞样品中Lyc-Prx Ⅳ的表达情况,并通过测定细胞内过氧化氢浓度来评价Lyc-Prx IV的体内抗氧化作用.结果显示,Lyc-Prx Ⅳ可在转染重组质粒p CMV-Lyc-Prx Ⅳ的HEK-293T细胞中表达,且细胞中A560处的吸光值在转染后6、12、24 h后分别为0.154、0.116以及0.162,而瞬时转染空载体p CMV后细胞样品在A560处的吸光值在转染后一直稳定在0.260左右,说明细胞中过氧化氢的浓度在转染后6、12、24 h时明显低于瞬时转染空载体p CMV细胞中的过氧化氢浓度（p〈0.01）.表明Lyc-Prx Ⅳ在生物体内可以分解过氧化氢,参与生物体内氧化还原状况的调控. |
| 关 键 词： | 海洋生物学 大黄鱼 过氧化物酶Ⅳ 细胞表达 过氧化氢 |
Expression and antioxidant activity analysis of Pseudosciana crocea peroxiredoxin IV in human embryonic kidney cells |
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| WANG Xian-hui,MU Yin-nan,NIE Pin,CHEN Xin-hua,AO Jing-qun.Expression and antioxidant activity analysis of Pseudosciana crocea peroxiredoxin IV in human embryonic kidney cells[J].Journal of Oceanography In Taiwan Strait,2014(4):492-498. | |
| Authors: | WANG Xian-hui MU Yin-nan NIE Pin CHEN Xin-hua AO Jing-qun |
| Institution: | WANG Xian-hui,MU Yin-nan,NIE Pin,CHEN Xin-hua,AO Jing-qun( 1. College of Fisheries, Jimei University, Xiamen 361021, China;2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, China) |
| Abstract: | To investigate the in vitro antioxidant activity of large yellow croaker（Pseudosciana crocea） Peroxiredoxin IV（Lyc-Prx IV）,we constructed a recombinant plasmid p CMV-Lyc-Prx IV to transiently transfect the HEK-293 T cells.The expression of Lyc-Prx IV in HEK-293 T cells was detected by Western blotting.The antioxidant activity of Lyc-Prx IV was evaluated by the quantification of the amount of intracellular hydrogen peroxide.The results showed that the Lyc-Prx IV was correctly expressed in HEK-293 T cells which transfected with p CMV-Lyc-Prx IV.The absorbance at 560 nm of cell samples at 6,12,and 24 h after transfected with p CMV-Lyc-Prx IV was 0.154,0.116 and0.162,respectively.While that of cell samples transfected with p CMV remained at 0.260 at different time after transfection.This result demonstrated the concentration of hydrogen peroxide in cells that transfected with p CMV-Lyc-Prx IV was considerably lower than that in cells transfected with pCMV at 6,12 and 24 h after transfection.These results indicate that the Lyc-Prx IV can disintegrate the hydrogen peroxide in cells,thus participating in the control of intracellular oxidation-reduction status. |
| Keywords: | marine biology Pseudosciana crocea peroxiredoxin IV cell expression hydrogen peroxide |
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