| 甲基磺酸乙酯对小新月菱形藻的生物学效应 |
| |
| 引用本文： | 杨茂纯,赵耕毛,王长海.甲基磺酸乙酯对小新月菱形藻的生物学效应[J].海洋科学,2015,39(1):8-12. |
| |
| 作者姓名： | 杨茂纯 赵耕毛 王长海 |
| |
| 作者单位： | 南京农业大学 资源与环境科学学院，江苏省海洋生物学重点实验室，江苏 南京 210095 |
| |
| 基金项目： | 国家863 计划项目(2012AA021706) |
| |
| 摘 要： | 为提高小新月菱形藻(Nitzschia closterium)的产油脂能力、获得油脂含量高的突变株,利用甲基磺酸乙酯(EMS)对该藻株进行了化学诱变处理,并对其生物学作用进行了考察。结果显示,EMS对小新月菱形藻具有较好的诱变作用,经0.1 mol/L和0.05 mol/L EMS处理后分别得到油脂生产能力较强的突变藻株YA和YB,在同等培养条件下两株突变藻单位体积细胞的内叶绿素a、类胡萝卜素、蛋白质、可溶性糖和油脂相对含量分别比对照组提高了30.05%和25.77%、22.15%和19.26%、9.06%和8.27%、48.25%和45.48%、44.06%和12.86%。
|
| 关 键 词： | 微藻 小新月菱形藻(Nitzschia closterium) 诱变 甲基磺酸乙酯 油脂 |
| 收稿时间： | 1/9/2014 12:00:00 AM |
| 修稿时间： | 2014/5/23 0:00:00 |
The biological effect of the Ethyl Methanesulphonate on Nitzschia closterium |
| |
| YANG Mao-chun
,
ZHAO Geng-mao
,
WANG Chang-hai.The biological effect of the Ethyl Methanesulphonate on Nitzschia closterium[J].Marine Sciences,2015,39(1):8-12. |
| |
| Authors: | YANG Mao-chun ZHAO Geng-mao WANG Chang-hai |
| |
| Abstract: | In order to obtain the high-lipid producing strains of Nitzschia closterium, the method of chemical mutation with Ethyl Methanesulphonate (EMS) was used, and the biological effects of EMS on the cells of N. closterium were studied. The results showed that the EMS has good mutagenesis effect on the cells of N. closterium, and two different mutant strains of N. closterium YA and YB were obtained after treatment with 0.1 mol/L and 0.05 mol/L dosages of EMS, respectively, which both had stronger oil production capacity. Under the same cultivation condition, the chlorophyll a, carotenoids, protein, soluble sugar contents and lipid relative contents of YA and YB have increased by 30.05% and 25.77%, 22.15% and 19.26%, 9.06% and 8.27%, 48.25% and 45.48%, and 44.06% and 12.86% compared with the CK, respectively. |
| |
| Keywords: | Microalgae Nitzschia closterium Mutagenesis Ethyl Methanesulphonate lipid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋科学》浏览原始摘要信息 |
| 点击此处可从《海洋科学》下载免费的PDF全文 |
|
