EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS     

梁英麦康森  孙世春于道展 《中国海洋湖沼学报》2001,19(3):249-254

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.  相似文献   

Total Lipid and Fatty Acid Classes in Decomposing Mangrove Leaves of <Emphasis Type="Italic">Bruguiera gymnorrhiza</Emphasis> and <Emphasis Type="Italic">Kandelia candel</Emphasis>: Significance with respect to Lipid Input     

Prosper?L.?Mfilinge  Tarik?Meziane  Zainudin?Bachok  Makoto?TsuchiyaEmail author 《Journal of Oceanography》2005,61(3):613-622

Changes in the concentration of total lipid and fatty acids (FAs) during the decomposition of mangrove leaves were investigated by field experiments using yellow leaves of Bruguiera gymnorrhiza (L.) Lamk. and Kandelia candel (L.) Druce, in order to quantify mangrove contribution to lipid and fatty acid inputs to marine sediments. Total lipid and total FA in the fresh (green and yellow) and decomposing leaves of both species were significantly higher during winter than summer. During decomposition, total lipid content and FA concentration, in particular branched chain fatty acids (BrFAs) and bacterial fatty acids (BFAs), increased to a maximum concentration in 45 days during winter and in 17 days during summer. Lipids were lost faster in K. candel leaf detritus than in B. gymnorrhiza leaf detritus in which >90% of the total lipid original weight was lost during the summer experiment and <60% during the winter experiment. The changes in the concentrations of total lipids and FAs in the decomposing leaves also indicate that mangrove leaves are significant sources of fatty acids and probably other lipid compounds to estuarine ecosystems and that tidal waters transport the lipids and FAs adsorbed to particulate matter from mangroves to adjacent estuarine sediments and the ocean.  相似文献   

pH对眼点拟微绿球藻的生长、总脂含量及脂肪酸组成的影响   总被引：9，自引：5，他引：9  

王秀良  刘晨临  张学成 《海洋科学》2002,26(5):63-67

pH对眼点拟微绿球藻（Namnocholoropsis oculata）的生长、总脂含量及脂肪酸组成有明显影响。研究表明，眼点拟微绿球藻的生长对pH有较强的适应性，在pH6.2～9.8的范围内均能较好的生长，但在pH5.5的条件下生长受到较强的抑制，在不同pH条件下，在同一细胞周期内总脂含量及脂肪酸组成均有相同的变化规律，即总脂含量在对数初期略有下降然后逐渐上升，在稳定期达到最大值。16：0，18：1n9的含量在稳定期显著升高，而EPA的含量在稳定期明显下降，在对数初期，藻细胞在pH6.8的条件下含有较高的EPA含量，而在pH9.8的条件下，藻细胞的EPA含量明显降低。  相似文献   

光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究   总被引：1，自引：0，他引：1  

李荷芳  周汉秋 《海洋科学集刊》2001,(43):178-183

近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。 作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。  相似文献   

十四株海洋微藻脂肪酸组成的研究   总被引：5，自引：0，他引：5  

张小葵  麦康森  郭健  孙世春 《中国海洋大学学报(自然科学版)》2001,31(2):205-210

对 4个门的 14株 (红藻门 8株 ,甲藻门 1株 ,隐藻门 2株 ,绿藻门 3株 )海洋微藻进行了脂肪酸含量测定 ,微藻在确定的条件下生长 ,指数生长末期收获。结果表明 ,各门的微藻都有其独特的脂肪酸特征 :红藻中含有大量的 2 0 :4 n- 6和 2 0 :5n- 3,其中紫球藻 R2 5含量最高 ,占总脂肪酸的4 9.8% (AA占总脂肪酸的 2 0 .5% ,EPA占总脂肪酸的 2 9.3% )。隐藻的主要脂肪酸是 16 :0、18:1n- 9、18:3n- 3、18:4 n- 3、2 0 :5n- 3、2 2 :5n- 3。与其它甲藻有别的虫黄藻 ,18:4 n- 3含量很少并且不含EPA,其主要合成的是 16 :0、18:1n- 9和 2 2 :6 n- 3。 C16和 C18的不饱和脂肪酸是本实验研究的 3株绿藻的主要脂肪酸  相似文献   

三丁基锡对牡蛎吞噬细胞和保护酶系统活力的影响          下载免费PDF全文

李张伟  韩雅莉  李平 《海洋科学》2007,31(12):38-42

检测了受三丁基锡(tributyltin,TBT)污染的牡蛎的吞噬细胞活力、超氧化物歧化酶活性、过氧化氢酶活性、脂质过氧化作用等生理生化指标。实验结果表明,牡蛎血细胞的吞噬活力随着TBT浓度的增加而下降;对不同TBT浓度下牡蛎的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性测定发现,在较低浓度下(TBT质量浓度<10μg/L),SOD和CAT的酶活力被抑制,随着TBT浓度的增大,SOD和CAT的比活力也随着增大,但当TBT达到较高浓度时,SOD和CAT的比活力开始下降,而过氧化脂质(LPO)却随着TBT的浓度的增加而增大。  相似文献   

氮浓度对四株海洋绿藻总脂含量和脂肪酸组成的影响   总被引：6，自引：0，他引：6  

曹春晖  孙世春  麦康森  梁英 《海洋湖沼通报》2006,(3):79-84

本文用NaNO3作为氮源,分别作了氮浓度的五个水平对2属(小球藻属、裂丝藻属)的四株绿藻的生长率、总脂含量及主要脂肪酸组成的影响。C19、C97和C102的脂肪含量随N浓度的改变而有较大变化,其中C102在1.6m mol/L时脂肪含量达到最大值(24.8%)。C95的脂肪含量随氮浓度变化不大。同时,由氮浓度引起的平均生长率μ与总脂含量之间无明显相关关系。四株绿藻的EPA(20∶5n-3)和PUFAs(polyunsaturated fatty acids)含量随培养基中氮浓度的改变有较大变化但因种而异。C95和C97均在中等氮浓度时EPA含量达到最大值,分别为23.8%和27.4%。C19和C102在高氮浓度(40mmol/L)获得EPA的最大值,分别为25.7%和26.6%。  相似文献   

多氯联苯(Aroclor 1242)胁迫下鲤鱼肝脏组织氧化应激   总被引：1，自引：0，他引：1  

王仁君  王建国  丁宁  孙萌  高配科 《海洋与湖沼》2018,49(6):1286-1293

本文在实验室条件下通过静态水质染毒方式在生理生化和转录水平上探究了我国重要经济鱼种鲤鱼(Cyprinus carpioio)对多氯联苯Aroclor 1242的氧化应激:鲤鱼肝脏组织抗氧化酶[超氧化物歧化酶(SuperoxideDismutase,SOD)和过氧化氢酶(Catalase,CAT)]活性、脂质过氧化产物丙二醛(Malonydialdehyde,MDA)含量、相关抗氧化酶编码基因(Cu/Zn-SOD、Mn-SOD、CAT)、应激蛋白HSP70、转录因子Nrf2和芳烃受体AhR2基因表达。结果表明:Aroclor 1242胁迫下,鲤鱼肝脏组织抗氧化酶活性表现出低浓度促进高浓度抑制的趋势;肝脏组织丙二醛含量显著升高,其中390μg/L暴露组鲤鱼肝脏组织MDA含量在第6d高于对照组92.1%(P0.01),表明肝脏组织发生脂质过氧化,膜系统受到损伤;鲤鱼肝脏组织中Cu/Zn-SOD、Mn-SOD、CAT、HSP70、Nrf2和AhR2编码基因表达出现不同程度下调。本文从酶学和基因转录水平上揭示了Aroclor1242胁迫下鲤鱼肝脏组织细胞的氧化应激。  相似文献   

Response of Microbial Communities in Soil to Multi-level Warming in a Highland Barley System of the Lhasa River     

FU Gang  SUN Wei  LI Shaowei  ZHONG Zhiming 《资源与生态学报(英文版)》2019,10(4):373-378

No studies have examined the effect of experimental warming on the microbial biomass and community composition of soil in agricultural ecosystem on the Qinghai-Tibet Plateau. Thus it is unclear whether the influences of experimental warming on microbial communities in soil are related to warming magnitude in croplands on this Plateau. This study performed warming experiment (control, low- and high-level) in a highland barley system of the Lhasa River in May 2014 to examine the correlation between the response of microbial communities in soil to warming and warming magnitude. Topsoil samples (0-10 and 10-20 cm) were collected on September 14, 2014. Experimental warming at both low and high levels significantly increased soil temperature by 1.02 ℃ and 1.59 ℃, respectively at the depth of 15 cm. Phospho lipid fatty acid (PLFA) method was used to determine the microbial community in soil. The low-level experimental warming did not significantly affect the soil’s total PLFA, fungi, bacteria, arbuscular mycorrhizal fungi (AMF), actinomycetes, gram-positive bacteria (G⁺), gram-negative bacteria (G^-), protozoa, the ratio of fungi to bacteria (F/B ratio), and ratio of G⁺ to G^- (G⁺/G^- ratio) at the 0-10 and 10-20 cm depth. The low-level experimental warming also did not significantly alter the composition of microbial community in soil at the 0-10 and 10-20 cm depth. The high-level experimental warming significantly increased total PLFA by 74.4%, fungi by 78.0%, bacteria by 74.0%, AMF by 66.9%, actinomycetes by 81.4%, G⁺ by 67.0% and G^- by 74.4% at the 0-10 cm depth rather than at 10-20 cm depth. The high-level experimental warming significantly altered microbial community composition in soil at the 0-10 cm depth rather than at 10-20 cm depth. Our findings suggest that the response of microbial communities in soil to warming varied with warming magnitudes in the highland barley system of the Lhasa River.  相似文献   

温度、饵料种类对中华哲水蚤(Calanus sinicus)油脂积累与生长发育的影响     

周孔霖  孙松 《海洋与湖沼》2016,47(4):787-794

中华哲水蚤(Calanus sinicus)C5期幼体的油脂积累是种群在黄海冷水团中得以顺利度夏的关键过程。本研究对温度(10°C和19°C恒温,10—19°CⅠ和10—19°CⅡ变温)与饵料种类(硅藻饵料,自然饵料)双因子培养实验进行研究,探讨温度和饵料种类对中华哲水蚤油脂积累与生长发育的影响作用。结果表明,不同的温度和饵料种类对C5期幼体的油脂积累均有影响。C5期幼体在变温组的油脂积累是10°C组的31%—102%,是19°C组的1.8—6.1倍,低温有利于C5期幼体降低个体代谢消耗以增加油脂的积累。在恒温培养下,C5期幼体在硅藻饵料组的油脂积累是自然饵料组的2.8倍,硅藻饵料比自然饵料更有利于油脂的积累。雌体的体长和油囊体积均随着温度的升高而减小。与硅藻饵料相比,在自然饵料组中雌体的性腺发育速度更快,性腺成熟度更高(繁殖指数:58%—65%)。  相似文献