| White spot syndrome virus envelope protein VP124 involved in the virus infection |
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| 作者姓名： | ZHU Yanbing WU Chenglin YANG Feng |
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| 作者单位： | School of Biotechnology Engineering, Jimei University, Xiamen 361021, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省科技攻关项目 |
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| 摘 要： | White spot syndrome virus （WSSV） is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins （VP39, VP124 and VP187 ）, individually. And then they were injected intramuscularly into crayfish （Procambarus clarkii） to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.
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| 关 键 词： | 白斑病毒 蛋白质 抗体 感染 |
| 收稿时间： | 2007/8/20 0:00:00 |
| 修稿时间： | 2007/10/8 0:00:00 |
White spot syndrome virus envelope protein VP124 involved in the virus infection |
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| ZHU Yanbing,WU Chenglin,YANG Feng.White spot syndrome virus envelope protein VP124 involved in the virus infection[J].Acta Oceanologica Sinica,2008,27(4):130-136. |
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| Authors: | ZHU Yanbing WU Chenglin and YANG Feng |
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| Institution: | 1.School of Biotechnology Engineering, Jimei University, Xiamen 361021, China2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: | White spot syndrome virus(WSSV)is one of the major shrimp pathogens causing large economic losses to shrimp farming.In an attempt to identify the envelope proteins involved in the virus infection,purified WSSV virions were mixed with three antisera against WSSV envelope proteins(VP39,VP124 and VP187),individually.And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays.The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124,the crayfish mortalities were 100% and 60% on the 8th day postinfection,individually.The virus infection could be delayed or neutralized by antibody against the envelope pro-tein VP124.Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infec-tion.The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish.All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection. |
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| Keywords: | white spot syndrome virus envelope protein VP124 antibody infection neutralization assay |
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