| 东海赤潮异弯藻Heterosigma akashiwo的形态特征及其分子序列分析 |
| |
| 引用本文： | 何飘霞,王红霞,陆斗定,戴鑫烽,夏平.东海赤潮异弯藻Heterosigma akashiwo的形态特征及其分子序列分析[J].海洋学研究,2011(3):155-161. |
| |
| 作者姓名： | 何飘霞 王红霞 陆斗定 戴鑫烽 夏平 |
| |
| 作者单位： | 国家海洋局第二海洋研究所国家海洋局海洋生态系统与生物地球化学重点实验室; |
| |
| 基金项目： | “973”计划资助项目(2010CB428702); 国家海洋公益性行业科研专项经费资助项目(201005031) |
| |
| 摘 要： | 将采集自东海南麂列岛海域水样进行分离、纯化,从中得到1株针胞藻,将其初步鉴定为赤潮异弯藻Heterosigma akashiwo(以下称东海藻株),并使用光学和荧光显微镜对其形态特征进行了详尽描述,及通过rDNA ITS序列测定、系统发育进化树构建和Jukes-Cantor距离矩阵分析,研究了其分子进化关系.结果表明:...
|
| 关 键 词： | 赤潮异弯藻 形态特征 rDNA ITS分子序列 东海 |
Morphological characteristics and ITS sequence analysis of Heterosigma akashiwo from East China Sea |
| |
| HE Piao-xia,WANG Hong-xia,LU Dou-ding,DAI Xin-feng,XIA Ping.Morphological characteristics and ITS sequence analysis of Heterosigma akashiwo from East China Sea[J].Journal of Marine Sciences,2011(3):155-161. |
| |
| Authors: | HE Piao-xia WANG Hong-xia LU Dou-ding DAI Xin-feng XIA Ping |
| |
| Institution: | HE Piao-xia,WANG Hong-xia,LU Dou-ding*,DAI Xin-feng,XIA Ping(Second Institute of Oceanography,SOA,Laboratory of Marine Ecosystem and Biogeochemistry,Hangzhou 310012,China) |
| |
| Abstract: | Morphology and phylogenetics of an ECS strain(strain No.LAMB100718) were isolated from waters of Nanji Island,China is analyzed.The stepwise dilution method was employed for isolation of the single cell algae strain.The strain was cultured in f/2 medium with salinity of 28~31,temperature of 17~20 ℃,light-dark cycle of 12∶12 hours and photosynthetically active radiation(PAR)of 9.6~10.6 μmol/(m2·s).The morphological features were acquired with optical microscope and fluorescent microscope.And the molecular phylogenetic analysis was made according to phylogenetic tree and Jukes-Cantor distance matrix.The results show that the ECS strain(LAMB100718) is a single cell without cell wall but covered with a thin layer of colloid.The shape of the cell varies in ball,egg or long ellipse like "potato".The cellular dimension is similar to that of other Heterosigma akashiwo strains.Its mean cell length is(16.0±1.8)μm(range 12~20μm),and the mean cell width is(10.0±1.4) μm(range 7~13μm).The thickness varies greatly.The front end of the cell is often rounded and back-end is relatively thin.There is an oblique furrow on the ventral part of the cell.At 1/2(or 1/4~1/3) of the cell length subapically,there are two unequal flagella,which stretches out from the furrow,with the longer one swimming forward and the shorter one trailing behind.The cell nucleus is slightly rounded,located in the center of the cell.The chloroplasts are brown in color,discoid in shape,9~22 in number,and distributed all over the periphery of the cell.The ITS rDNA sequence of the strain LAMB100718 is 557 base pair and the GC content is 52.6%.Only one base pair is different from other four Heterosigma akashiwo strains(AB217657,AF134727,AF134728 and AY704164) in GeneBank.The phylogenetic tree shows that the Heterosigma akashiwo stain LAMB100718,AB217657,AF134727,AF134728 and AY704164 are clustered together,with a high bootstrap value(100%).The result from the Jukes-Cantor distance matrix suggests that the nucleotide divergence of the five strains is between 0.000~0.004,which belongs to intraspecific differences.According to the morphological and phylogenetic study,the ECS strain(LAMB100718) from waters of Nanji Islands,East China Sea can be confirmed as Heterosigma akashiwo.This strain was isolated for the first time from the East China Sea(ECS) and recorded for the first time in Zhejiang coastal waters. |
| |
| Keywords: | Heterosigma akashiwo morphology characteristic rDNA ITS East China Sea |
| 本文献已被 CNKI 等数据库收录！ |
|
