Comparative molecular analysis of bacterial communities inhabiting pristine and polluted with polycyclic aromatic hydrocarbons Black Sea coastal sediments |
| |
| Authors: | Nadezhda H Todorova Roumyana S Mironova Ventzislav K Karamfilov |
| |
| Institution: | 1. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Y. Gagarin 2 str., 1113 Sofia, Bulgaria;2. Institute of Molecular Biology “Roumen Tsanev”, Bulgarian Academy of Sciences, G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria |
| |
| Abstract: | Molecular analysis was applied to characterize bacterial community structure in sediment samples collected from pristine site and oil-polluted Black Sea harbor. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed a high similarity in the restriction patterns of both samples thus not demonstrating the effect of the pollutant on the structure of the bacterial communities. Constructed 16S rRNA gene libraries gave more detailed assessment of members. Results showed that α- and γ-Proteobacteria were dominant in the oil polluted site, whereas the pristine site was characterized by prevalence of Actinobacteria. The biodegradative potential of the adapted bacterial community in the oil-polluted sediments was demonstrated by the presence of the aromatic ring hydroxylating dioxygenase genes. |
| |
| Keywords: | Bacterial communities Oil pollution 16S rRNA gene Sediment PAH degradative potential ARDRA |
| 本文献已被 ScienceDirect 等数据库收录！ |
