Testing use of mitochondrial COI sequences for the identification and phylogenetic analysis of New Zealand caddisflies (Trichoptera) |
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| Authors: | Ian D Hogg Brian J Smith Jonathan C Banks Jeremy R Dewaard Paul D N Hebert |
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| Institution: | 1. Centre for Biodiversity and Ecology Research, Department of Biological Sciences , University of Waikato , Private Bag 3105, Hamilton, 3240, New Zealand E-mail: hogg@waikato.ac.nz;2. National Institute for Water and Atmospheric Research Limited , Hamilton, 3240, New Zealand;3. Centre for Biodiversity and Ecology Research, Department of Biological Sciences , University of Waikato , Private Bag 3105, Hamilton, 3240, New Zealand;4. Department of Forest Sciences, Forestry Sciences Centre , University of British Columbia , Vancouver, BC, V6T 1Z4, Canada;5. Royal British Columbia Museum Entomology , 675 Belleville Street, Victoria, BC, V8W 9W2, Canada;6. Biodiversity Institute , Ontario University of Guelph , Guelph, ON, NIG 2W1, Canada |
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| Abstract: | We tested the hypothesis that cytochrome c oxidase subunit 1 (COI) sequences would successfully discriminate recognised species of New Zealand caddisflies. We further examined whether phylogenetic analyses, based on the COI locus, could recover currently recognised superfamilies and suborders. COI sequences were obtained from 105 individuals representing 61 species and all 16 families of Trichoptera known from New Zealand. No sequence sharing was observed between members of different species, and congeneric species showed from 2.3 to 19.5% divergence. Sequence divergence among members of a species was typically low (mean = 0.7%; range 0.0–8.5%), but two species showed intraspecific divergences in excess of 2%. Phylogenetic reconstructions based on COI were largely congruent with previous conclusions based on morphology, although the sequence data did not support placement of the purse‐cased caddisflies (Hydroptilidae) within the uncased caddisflies, and, in particular, the Rhyacophiloidea. We conclude that sequence variation in the COI gene locus is an effective tool for the identification of New Zealand caddisfly species, and can provide preliminary phylogenetic inferences. Further research is needed to ascertain the significance of the few instances of high intra‐specific divergence and to determine if any instances of sequence sharing will be detected with larger sample sizes. |
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| Keywords: | aquatic insects Arthropoda barcoding mtDNA phylogeny systematics taxonomy |
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