| 青岛近岸特征环境中海洋异养细菌的分布规律及其分子鉴定 |
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| 引用本文： | 李筠,周宏霞,刘佳琳,张跃峰,陈吉祥,张晓华.青岛近岸特征环境中海洋异养细菌的分布规律及其分子鉴定[J].中国海洋大学学报(自然科学版),2006,36(6):965-970. |
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| 作者姓名： | 李筠 周宏霞 刘佳琳 张跃峰 陈吉祥 张晓华 |
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| 作者单位： | 中国海洋大学生命科学与技术学部,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;英国达尔文国际合作项目 |
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| 摘 要： | 研究海洋微生物特征,从青岛近岸养殖密集区、工业区、洁净区分离了428株海洋异养细菌,采用BIOLOG技术对其进行了鉴定和聚类,从中选出13株具有代表性的菌株,克隆其16SrDNA片段,进行分子鉴定。结果表明,它们分别属于弧菌属(Vibrio)、不动杆菌属(Acinetobacter)、假交替单胞菌属(Pseudoalteromonas)、假单胞菌属(Pseudomonas)、海单胞菌属(Oceanimonas)、噬纤维菌属(Cellulophaga)、赤细菌属(Erythrobacter)和Rhodovirga。在3种特征环境中,海洋异养细菌的分布具有一定的规律性:其中洁净区的特征类群属于弧菌属(Vibrio),工业区的特征类群属于弧菌属(Vibrio)和不动杆菌属(Acinetobacter),养殖密集区的特征类群属于弧菌属(Vibrio)、假交替单胞菌属(Pseudoalteromonas)、假单胞菌属(Pseu-domonas)、海单胞菌属(Oceanimonas)、赤细菌属(Erythrobacter)、噬纤维菌属(Cellulophaga)和噬冷菌属(Algoriphagus)。
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| 关 键 词： | 海洋异养细菌 分子分类鉴定 |
| 文章编号： | 1672-5174(2006)06-965-06 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-04-29 |
Distribution and Identification of Marine Heterotrophic Bacteria in Different Sea Areas Close to the Shore of Qingdao |
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| LI Yun,ZHOU Hong-Xia,LIU Jia-Lin,ZHANG Yue-Feng,GHEN Ji-Xiang,ZHANG Xiao-Hua.Distribution and Identification of Marine Heterotrophic Bacteria in Different Sea Areas Close to the Shore of Qingdao[J].Periodical of Ocean University of China,2006,36(6):965-970. |
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| Authors: | LI Yun ZHOU Hong-Xia LIU Jia-Lin ZHANG Yue-Feng GHEN Ji-Xiang ZHANG Xiao-Hua |
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| Institution: | Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Four hundred and twenty eight strains of heterotrophic bacteria were isolated from marine sediment,seawater,seaweed,fish,and shellish,coming from different sea areas close to the shore of Qingdao.The sampling locations belonged respectively to industrially and aquaculturally affected regions and clean regions.The BIOLOG technique was adopted for the identification and clustering of these isolates.Thirteen representative isolates were selected from these isolates,whose genera were identified and classified based on an analysis of 16SrDNA sequences.The results indicated that they belong to Vibrio,Acinetobacter,Pseudoalteromonas,Pseudomonas,Oceanimonas,Cellulophaga,Erythrobacter and Algoriphagus.The distributions of bacteria from the three regions possess certain regularity.The results are as follows: The peculiar community in the clean region belonged to Vibrio.The peculiar communities in the industrially regions belonged to Vibrio and Acinetobacter.The peculiar communities in the aquaculturally affected regions belonged to Vibrio,Pseudoalteromonas,Algoriphagus,Pseudomonas,Oceanimonas,Erythrobacter and Cellulophaga. |
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| Keywords: | 16SrDNA |
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