| 细胞外钙和钾离子对调节离体鲤脑垂体促性腺激素分泌的影响 |
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| 引用本文： | 林信伟,李英文,林浩然.细胞外钙和钾离子对调节离体鲤脑垂体促性腺激素分泌的影响[J].海洋与湖沼,1995,26(3):295-301. |
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| 作者姓名： | 林信伟 李英文 林浩然 |
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| 作者单位： | 中山大学生物系!广州，510275，中山大学生物系!广州，510275，中山大学生物系!广州，510275 |
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| 基金项目： | 高等学校博士点科学基金，加拿大国际发展研究中心资助!3－p－83－1101 |
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| 摘 要： | 作者于１９９０年９月－１９９１年１月采用离体灌流培育方法对鲤鱼脑垂体促性激素的分泌调节进行研究。结果表明，脑垂体基础促性腺激素（ＧｔＨ）分泌不依赖于细胞外钙离子（Ｃａ＾２＋）；但脉冲式鲑鱼促性腺激素释放激素（ｓＧｎＲＨ）刺激的ＧｔＨ分泌，则依赖于细胞外Ｃａ＾２＋且细胞外Ｃａ＾２＋的作用至少部分通过电位敏感性Ｃａ＾２＋通道。５０ｍｍｏｌ／Ｌ高浓度钾离子（Ｋ＾＋）显著刺激脑垂体基础ＧｔＨ分泌，Ｋ＾＋的
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| 关 键 词： | 鲑鱼 促性腺激素 钙离子 钾离子 鲤鱼 |
| 收稿时间： | 1992/7/18 0:00:00 |
| 修稿时间： | 1992/10/30 0:00:00 |
EFFECTS OF EXTRACELLULAR CALCIUM AND POTASSIUM ON THE`. REGULATION OF GONADOTROPIN SECRETION FROM FITUITARY OF COMMON CARP IN VITRO |
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| Lin Xinwei,Li Yingwen and Lin Haoran.EFFECTS OF EXTRACELLULAR CALCIUM AND POTASSIUM ON THE`. REGULATION OF GONADOTROPIN SECRETION FROM FITUITARY OF COMMON CARP IN VITRO[J].Oceanologia Et Limnologia Sinica,1995,26(3):295-301. |
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| Authors: | Lin Xinwei Li Yingwen and Lin Haoran |
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| Institution: | Depariment of Biology. Zhongshan University. Guangzhou 510275 |
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| Abstract: | The effects of extracellular Ca2+ and K+ on the basal and salmon GnRH (sGn-RH)-stimulated gonadotropin (GtH) release from pituitary of the common carp (Cyprinut carpio) were studied by using an in uitro perifusion system for pituitary fragments. The basal GtH release from the common carp's perifused pituitary extracellular Ca2+-independent. The GtH release from the common carp's perifused pituitary in response to 2 min pulses of sGnRH (1, 10, 100n mol/L) was extracellular Ca2+dependent; in the absence of extracellular Ca2+ or the reduction in Ca2+ concentration,the significant decreases in the GtH release responses to sGnRH were observed.Voltagesensitive calcium chaunels (VSCC) and antagonist verapamil (VER) dosedependently and significantly inhibited GtH release responses to 2 min pulses of sGnRH,in a VER was ineffective on the basal GtH secretion. These results indicate that tlie effect of extracellular Ca2+ on the GtH-releasing action of sGnRH is, at least, partially through the action of VSCC. 50 m mol/L K+ significantly stimulated the basal GtH release from the perifused pituitary of the common carp, and the action of K+ was time-and extracellular Ca2+-dependent. Moreover, no significant influence of increaaed concentration of K+ on sGnRH-induced GtH release was observed. |
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| Keywords: | Salmon GnRH Conadotropin Calcium Potassium Perifusion Common carp |
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