| 河流弧菌(Vibrio fluvialis)对牙鲆(Paralichthys olivaceus)粘液的趋化和粘附作用 |
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| 引用本文： | 鄢庆枇,邹文政,纪荣兴,义家波.河流弧菌(Vibrio fluvialis)对牙鲆(Paralichthys olivaceus)粘液的趋化和粘附作用[J].海洋与湖沼,2008,39(4). |
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| 作者姓名： | 鄢庆枇 邹文政 纪荣兴 义家波 |
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| 作者单位： | 姜大学水产学院,福建省高校水产科学技术与食品安全重点实验室,厦门,3610211 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省科技攻关项目，厦门市海洋与渔业局科技项目 |
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| 摘 要： | 采用生长曲线法测定了河流弧菌在牙鲆表皮、鳃和肠粘液中的生长情况，结果表明，河流弧菌在3种粘液中都能生长，生长前期符合Logistic模型，然后进入Gompertz模型，在鳃粘液中的生长量高于肠粘液和表皮粘液。采用毛细管法研究了河流弧菌对牙鲆3种粘液的趋化作用，结果表明，河流弧菌对牙鲆3种粘液具有较强的趋化应答，在粘液总蛋白含量相同的情况下，河流弧菌对牙鲆鳃粘液的趋化应答显著强于对表皮粘液的趋化应答（P〈0．05），而对肠粘液的趋化应答极显著低于对鳃粘液和表皮粘液的趋化应答（P〈0．01）。采用^3H—TdR示踪法进行了河流弧菌对牙鲆3种粘液的粘附作用研究，结果表明，河流弧菌对牙鲆表皮粘液和鳃粘液的粘附率较高，而对肠粘液的粘附率较低，特别是孵育30、60和90min时河流弧菌对牙鲆表皮粘液和鳃粘液的粘附率显著高于对肠粘液的粘附率（P〈0．05）。这些结果表明，河流弧菌能够在牙鲆粘液，尤其是鳃粘液中生长和定植，有利于其进一步感染、致病。
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| 关 键 词： | 河流弧菌 牙鲆 粘液 趋化作用 粘附作用 |
THE CHEMOTAXIS AND ADHESION OF VIBRIO FLUVIALIS TO MUCUS OF PARALICHTHYS OLIVA CEUS |
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| YAN Qing-Pi,ZOU Wen-Zheng,JI Rong-Xing,YI Jia-Bo.THE CHEMOTAXIS AND ADHESION OF VIBRIO FLUVIALIS TO MUCUS OF PARALICHTHYS OLIVA CEUS[J].Oceanologia Et Limnologia Sinica,2008,39(4). |
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| Authors: | YAN Qing-Pi ZOU Wen-Zheng JI Rong-Xing YI Jia-Bo |
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| Abstract: | The growth of Vibrio fluvialis in skin, gill and intestinal mucus of Paralichthys olivaceus, the chemotactic response and adhesion of V. fluvialis to the mucus were investigated. The results show that, V. fluvialis grew well in all the mucus in Logistice growth model at prophase and Gompertz growth model at anaphase, the maximum biomass yield in gill mucus was higher than those in skin and intestinal mucus; V. fluvialis exhibited strong chemotactic response towards the mucus from the three organs, the chemotaxis to gill mucus was significantly stronger than to skin mucus (P<0.05), and extremely significant lower (P<0.01) to intestinal mucus than those to gill mucus and skin mucus. The bacteria adhesion to skin mucus and gill mucus were significantly higher (P<0.05) than that to intestinal mucus after incubation for 30, 60 and 90 min. Therefore, pathogenic V. fluvialis could grow and colonize in mucus of P. olivaceus, especially in gill mucus, causing further infection. |
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| Keywords: | Vibrio fluvialis Paralichthys olivaceus Mucus Chemotaxis Adhesion |
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