| 基于18S和ITS-5.8S rDNA基因序列的白色霞水母(Cyanea nozakii)的分子鉴定与检测 |
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| 引用本文： | 李玉龙,董婧,王彬,孙明,王爱勇,王文波.基于18S和ITS-5.8S rDNA基因序列的白色霞水母(Cyanea nozakii)的分子鉴定与检测[J].海洋与湖沼,2016,47(1):158-165. |
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| 作者姓名： | 李玉龙 董婧 王彬 孙明 王爱勇 王文波 |
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| 作者单位： | 辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023,辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023,辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023,辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023,辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023,辽宁省海洋水产科学研究院 辽宁省海洋生物资源与生态学重点实验室 大连 116023 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）,海洋公益性行业专项 |
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| 摘 要： | 采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。
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| 关 键 词： | 白色霞水母 18S rDNA ITS-5.8S 分子鉴定 系统分析 |
| 收稿时间： | 2015/2/10 0:00:00 |
| 修稿时间： | 2015/8/20 0:00:00 |
MOLECULAR IDENTIFICATION AND DETECTION OF CYANEA NOZAKII BY PARTIAL SEQUENCING OF 18S RDNA AND ITS-5.8S |
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| LI Yu-Long,DONG Jing,WANG Bin,SUN Ming,WANG Ai-Yong and WANG Wen-Bo.MOLECULAR IDENTIFICATION AND DETECTION OF CYANEA NOZAKII BY PARTIAL SEQUENCING OF 18S RDNA AND ITS-5.8S[J].Oceanologia Et Limnologia Sinica,2016,47(1):158-165. |
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| Authors: | LI Yu-Long DONG Jing WANG Bin SUN Ming WANG Ai-Yong and WANG Wen-Bo |
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| Institution: | Liaoning Ocean and Fisheries Science research Institute,Liaoning Ocean and Fisheries Science research Institute,Liaoning Ocean and Fisheries Science research Institute,Liaoning Ocean and Fisheries Science research Institute,Liaoning Ocean and Fisheries Science research Institute,Liaoning Ocean and Fisheries Science research Institute |
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| Abstract: | In this study, the partial sequences of nuclear 18S(1728bp) and ITS-5.8S(667bp) rDNA were determined from polyp, ephyra and adult of the Cyanea nozakii. The homologous sequences of other Cyanea jellyfishes which collected from GenBank were also included in this study. The genetic information indexes, including sequence variation and Kimura-2 parameter genetic distance were examined. It was found that the ITS-5.8S sequences exhibit 0% to 0.8% nucleotide divergence between Cyanea nozakii and uncultured eukaryote species from GenBank, suggesting that the uncultured eukaryote species was Cyanea nozakii. Total of the 1709 aligned base pairs of 18S rDNA with 33 variable sites were detected in the analyzed five Cyanea jellyfishes. In addition, 368 aligned base pairs of ITS1 sequences, with 203 variable sites, 178 parsimony informative sites, 21 singleton sites, were detected in the analyzed four Cyanea jellyfishes. The average genetic distances among species and individuals based on the 18S rDNA and ITS1 were 0.008 and 0, 0.284 and 0.019, respectively. Phylogenetic trees were constructed based on Neighbor-Joining method. According to the phylogenetic trees, different individuals of the same species, different species of the same family were grouped together. These results show that ITS1 is highly divergent among different Cyanea jellyfishes and could be an appropriate marker for the species identification, young jellyfish detection, and molecular systematic studies at the species and population levels. |
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| Keywords: | Cyanea nozakii 18S rDNA ITS-5 8S rDNA molecular identification phylogenetic analysis |
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