| 莱州湾近岸海域浮游纤毛虫群落组成和季节变化 |
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| 引用本文： | 李雯璐,徐大鹏,季道德.莱州湾近岸海域浮游纤毛虫群落组成和季节变化[J].海洋与湖沼,2022,53(6):1441-1454. |
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| 作者姓名： | 李雯璐 徐大鹏 季道德 |
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| 作者单位： | 烟台大学海洋学院 山东烟台 264005;厦门大学近海海洋环境与科学国家重点实验室 海洋与地球学院 海洋微型生物与地球圈层研究所 福建厦门 361104 |
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| 基金项目： | 中国国家自然科学基金资助项目,31772413号,41876142号。 |
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| 摘 要： | 浮游纤毛虫是真核微型生物的重要类群,在浮游生态系统中发挥着重要作用。利用传统形态学与高通量测序技术相结合的方法,于2020年9月至2021年8月对莱州湾三山岛近岸浮游纤毛虫进行每月两次的调查。对环境DNA的18S rRNA基因的V4区进行高通量测序,结果显示无壳寡毛类(占纤毛虫序列丰度的40.14%)和砂壳纤毛虫(10.67%)是优势类群,并且具有明显的季节变化。无壳寡毛类在冬季序列相对丰度最高,主要由急游虫属(Strombidium)和急游虫科未定类群(Strombidiidae_X)组成;砂壳类在春夏季序列相对丰度最高,主要由砂壳目未定类群(Tintinnida_XX)和拟铃虫属(Tintinnopsis)组成。浮游纤毛虫群落的β多样性呈现显著的季节变化,而α和β多样性在站点间差异均不显著。与环境因子的RDA分析显示,总有机碳和溶解氧含量是影响纤毛虫群落结构的主要环境驱动因子。形态学方法共检获鉴定17种纤毛虫,分别隶属于7个属,其中砂壳类在多样性和检获频率上均为最优势类群。研究填补了莱州湾三山岛海域浮游纤毛虫的多样性、群落结构和季节动态研究的空白,同时为深入研究温带海湾近岸浮游纤毛虫群落的长期变化规律以及对环境因子的响应机制提供本底资料。
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| 关 键 词： | 浮游纤毛虫 多样性 近岸海域 高通量测序 形态学 |
| 收稿时间： | 2022/4/28 0:00:00 |
| 修稿时间： | 2022/5/12 0:00:00 |
COMMUNITY COMPOSITION AND SEASONAL VARIATION OF PLANKTONIC CILIATES IN SANSHAN ISLAND, LAIZHOU BAY |
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| LI Wen-Lu,XU Da-Peng,JI Dao-De.COMMUNITY COMPOSITION AND SEASONAL VARIATION OF PLANKTONIC CILIATES IN SANSHAN ISLAND, LAIZHOU BAY[J].Oceanologia Et Limnologia Sinica,2022,53(6):1441-1454. |
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| Authors: | LI Wen-Lu XU Da-Peng JI Dao-De |
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| Institution: | School of Ocean, Yantai University, Yantai 264005, China;State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Science, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen 361104, China |
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| Abstract: | Planktonic ciliates are important eukaryotic microorganisms and play a crucial role in the planktonic ecosystem. Semimonthly ecological research was conducted on planktonic ciliates in Sanshan Island, Laizhou Bay from September 2020 to August 2021 combining traditional morphology and high-throughput sequencing technology (HTS). HTS for the V4 region of the 18S rRNA gene showed that aloricate oligotrichs (accounting for 40.14% of the abundance of ciliate sequences) and tintinnids (10.67%) were dominant groups with obvious seasonal variation. The relative abundance of oligotrichs consisted of mainly Strombidium and Strombidiidae_X, and was the highest in winter. The relative abundance of tintinnids was the highest in spring and summer, and they were composed of mainly Tintinnida_XX and Tintinnopsis. Beta diversity showed significant seasonal variation, but both alpha diversity and beta diversity showed no considerable difference in spatial distribution. Redundancy analysis demonstrated that total organic carbon and dissolved oxygen content were the significant environmental factors on the seasonal variation of planktonic ciliates in the coastal water of Sanshan Island. Seventeen species were identified in microscopy method, and they belong to 7 genera. Tintinnids were the most dominant group in diversity and occurrence. This work filled the gaps in species diversity, community structure, and seasonal dynamic of planktonic ciliates in Sanshan Island, Laizhou Bay. Meanwhile, this study provided essential data for future studies on the long-term variation of planktonic ciliates community in the coastal waters of temperate bays and their response mechanisms to environmental factors. |
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| Keywords: | planktonic ciliates diversity coastal water High-throughput sequencing morphology |
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