| 中国对虾血细胞及其免疫研究 |
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| 引用本文： | 李光友,王青.中国对虾血细胞及其免疫研究[J].海洋与湖沼,1995,26(6):591-597. |
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| 作者姓名： | 李光友 王青 |
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| 作者单位： | 中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071 |
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| 基金项目： | 中国科学院“八五”重大项目!A0892081312 |
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| 摘 要： | 于１９９１年３月－１９９２年４月青岛近海及一养虾场捕集中国对虾亲虾和越冬亲虾，以扫描电镜和透射电镜观察中国虾的血细胞形态及其亚显微结构；研究定量测定细胞吞噬活性的方法。结果表明，中国对虾血细胞分３种：无颗粒细胞，呈球形或梭形，胞质不含颗粒粒；小颗粒细胞及颗粒细胞，卵球形或椭球球形，胞质含有电子致密颗粒，颗粒可分均匀、非均匀的；小颗粒细胞质富含线粒体，可能与它对异物敏感易发生胞吐作用有关，该细胞是识
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| 关 键 词： | 对虾 中国对虾 血细胞 形态 亚显微结构 免疫 |
| 收稿时间： | 5/1/1992 12:00:00 AM |
| 修稿时间： | 1995/5/20 0:00:00 |
STUDIES ON HAEMOCYTES AND CELLULAR IMMUNITY IN PRAWN PENAEUS CHINENSIS |
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| Li Guangyou and Wangqing.STUDIES ON HAEMOCYTES AND CELLULAR IMMUNITY IN PRAWN PENAEUS CHINENSIS[J].Oceanologia Et Limnologia Sinica,1995,26(6):591-597. |
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| Authors: | Li Guangyou and Wangqing |
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| Institution: | Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071 |
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| Abstract: | This paper reports studies on the morphology, submicroscopic structure and thequantitive method of testing phagocytic activity of P. chinensis collected in Qingdaocoast from March 1991 to April 1992.Results showed that P. chinensis has three distinct populations of circulatinghaemocytes: hyaline cells, semigranu1ar cells, and granular cells; the hyaline cells areround without cytoplasmic granules inside; the semigranulsr cells and the granularcel1s are ovold or ellipsoid and contain cytoplasmic granules; the semigranular cellsplay critical roles in recognizing foreigs objects and have plenty of mitochondria,which is perhaps related to their sensitivity to foreign particles and easy degranula-tion. Golgi apparatus probably associated with the forming of granules can be seenin the granular cells.Gimesa stain results showed that the above three kinds of cells were purplishred, and the nuclei and granules were much deeper, purplish blue, and their beingsimilar to human granular cells. That indicates they have similar functions in hostdefence reactions.The Luminol-enhanced-chemiluminescen (CL) method widely applied in stu-dying phagocytosis kinetics wasused to test phagocytic activity of haemocytes quan-titively. The result of CL response, counts per minute (cpm), can be read from aBeckman scintillation counter. The cpm of the haemocytes mixed with lipopolysac-charide (LPS) and Vibrio suspension were higher than the cpm of haemocyteswith only Vibrio suspension and the cpm of the conttol. It is suggested that LPScan stimulate and enhance the phagocytosis bf blood cells in P. chinensis. Zymosaninduced wear phagocytosis. This method can give quantitve criterion to monitor theimmune function of prawns and will have many applications in aquaculture. |
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| Keywords: | Penaeus chinensis Haemocytes Morphology Submicroscopic structure Immune Method |
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