| 基于微卫星(SSR)分子标记的中国对虾渤海湾增殖亲虾群体及回捕群体的遗传多样性分析 |
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| 引用本文： | 王陌桑,王伟继,肖广侠,刘克奉,胡玉龙,田涛,孔杰,金显仕.基于微卫星(SSR)分子标记的中国对虾渤海湾增殖亲虾群体及回捕群体的遗传多样性分析[J].海洋学报(英文版),2016,35(8):51-56. |
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| 作者姓名： | 王陌桑 王伟继 肖广侠 刘克奉 胡玉龙 田涛 孔杰 金显仕 |
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| 作者单位： | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 20130, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Bohai Fisheries Research Institute of Tianjin, Tianjin 300457, China,Bohai Fisheries Research Institute of Tianjin, Tianjin 300457, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 20130, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| 基金项目： | The National Basic Research Program of China (973 Program) Adaptive Response of Fishery Species to Environmental Changes and Their Effects on Population Dynamics under contract No. 2015CB453303; the International Science & Technology Cooperation Program of China under contract No. 2013DFA31410. |
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| 摘 要： | Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles(N_a) were 8–63 and 6–60, respectively. The numbers of effective alleles(N_e) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity(H_o) values(0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity(H_e) values(0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests(2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. F_(is) values were positive at seven loci and the inbreeding coefficients(F) of the two populations estimated by trio ML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F_(st) values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes(3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014,the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed.
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| 关 键 词： | Fenneropenaeus chinensis SSR genetic diversity inbreeding effective population size |
| 收稿时间： | 7/1/2015 12:00:00 AM |
| 修稿时间： | 2015/11/30 0:00:00 |
Genetic diversity analysis of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay based on an SSR marker |
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| WANG Mosang,WANG Weiji,XIAO Guangxi,LIU Kefeng,HU Yulong,TIAN Tao,KONG Jie and JIN Xianshi.Genetic diversity analysis of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay based on an SSR marker[J].Acta Oceanologica Sinica,2016,35(8):51-56. |
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| Authors: | WANG Mosang WANG Weiji XIAO Guangxi LIU Kefeng HU Yulong TIAN Tao KONG Jie and JIN Xianshi |
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| Institution: | 1.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 20130, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China3.Bohai Fisheries Research Institute of Tianjin, Tianjin 300457, China |
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| Abstract: | Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles (Na) were 8-63 and 6-60, respectively. The numbers of effective alleles (Ne) were 2.52-21.60 and 2.67-20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity (Ho) values (0.638-0.910 and 0.712-0.927) were lower than the expected heterozygosity (He) values (0.603-0.954 and 0.625-0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests (2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. Fis values were positive at seven loci and the inbreeding coefficients (F) of the two populations estimated by trioML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. Fst values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes (3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014, the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed. |
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| Keywords: | Fenneropenaeus chinensis SSR genetic diversity inbreeding effective population size |
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