| Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans |
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| 作者姓名： | ZENG Yinxin LI Huirong YU Yong CHEN Bo ZHENG Tianling |
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| 作者单位： | [1]School of Life Sciences, Xiamen University, Xiamen 361005, China [2]Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家自然科学基金
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and the National Science and Technology Ministry of China under |
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| 摘 要： | A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level.
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| 关 键 词： | 浮游细菌 多样性 北极地区 南极地区 |
| 收稿时间： | 2006/4/14 0:00:00 |
| 修稿时间： | 2006-04-142006-07-22 |
Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans |
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| ZENG Yinxin,LI Huirong,YU Yong,CHEN Bo,ZHENG Tianling.Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans[J].Acta Oceanologica Sinica,2007,26(4):93-103. |
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| Authors: | ZENG Yinxin LI Huirong YU Yong CHEN Bo and ZHENG Tianling |
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| Institution: | 1.School of Life Sciences, Xiamen University, Xiamen 361005, China;Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China2.Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China3.School of Life Sciences, Xiamen University, Xiamen 361005, China |
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| Abstract: | A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and β-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSw10175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus Shewanella dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria ( ＞ 99% ),but also between polar marine bacteria and bacteria from other aquatic environments ( ≥98.8% ) , including temperate ocean,deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. |
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| Keywords: | bacterioplankton diversity arctic antarctic oceans bipolar isolated phenotypic characterization diversity distribution species level global pond lake deep aquatic environments significant arctic and antarctic marine bacteria sequence above the Prydz Bay |
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